Supercritical Fluid Chromatography

Dai, Jun; Zhang, Yingru; Wang‐Iverson, David; Tymiak, Adrienne A.

doi:10.1002/9781118180778.ch24

Cited by 2 publications

(1 citation statement)

References 122 publications

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“…When working with binary fluids at a constant pressure, an increase in temperature produces a decrease in density. In SFC, its effect is a controversial point: in some cases, an increase in temperature produces a decrease in retention, but in others, it leads to an increase, or even has no very significant effect on retention [32,47].…”

Section: Effect Of Temperaturementioning

confidence: 99%

Study of Different Chiral Columns for the Enantiomeric Separation of Azoles Using Supercritical Fluid Chromatography

et al. 2022

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The enantiomeric separation of antifungal compounds is an arduous task in pharmaceutical and biomedical fields due to the different properties that each diastereoisomer presents. The enantioseparation of a group of fungicides (sulconazole, bifonazole, triadimefon and triadimenol) using supercritical fluid chromatography was achieved in this work. For this goal, four different chiral columns based on polysaccharide derivatives, as well as the effect of different chromatographic parameters such as temperature, type and percentage of organic modifier (methanol, ethanol and isopropanol), were thoroughly investigated. The inversion of the elution order of enantiomers as a result of a change in the stationary phase or organic modifier was also evaluated by employing a circular dichroism detector. The best separation conditions, in terms of the enantioresolution and analysis time, were obtained with the Lux® Cellulose-2 column using isopropanol as the organic modifier.

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Section: Effect Of Temperaturementioning

confidence: 99%

Study of Different Chiral Columns for the Enantiomeric Separation of Azoles Using Supercritical Fluid Chromatography

et al. 2022

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Significance and challenges of stereoselectivity assessing methods in drug metabolism

Shen

Zeng

2016

Journal of Pharmaceutical Analysis

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Stereoselectivity in drug metabolism can not only influence the pharmacological activities, tolerability, safety, and bioavailability of drugs directly, but also cause different kinds of drug–drug interactions. Thus, assessing stereoselectivity in drug metabolism is of great significance for pharmaceutical research and development (R&D) and rational use in clinic. Although there are various methods available for assessing stereoselectivity in drug metabolism, many of them have shortcomings. The indirect method of chromatographic methods can only be applicable to specific samples with functional groups to be derivatized or form complex with a chiral selector, while the direct method achieved by chiral stationary phases (CSPs) is expensive. As a detector of chromatographic methods, mass spectrometry (MS) is highly sensitive and specific, whereas the matrix interference is still a challenge to overcome. In addition, the use of nuclear magnetic resonance (NMR) and immunoassay in chiral analysis are worth noting. This review presents several typical examples of drug stereoselective metabolism and provides a literature-based evaluation on current chiral analytical techniques to show the significance and challenges of stereoselectivity assessing methods in drug metabolism.

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Supercritical Fluid Chromatography

Cited by 2 publications

References 122 publications

Study of Different Chiral Columns for the Enantiomeric Separation of Azoles Using Supercritical Fluid Chromatography

Study of Different Chiral Columns for the Enantiomeric Separation of Azoles Using Supercritical Fluid Chromatography

Significance and challenges of stereoselectivity assessing methods in drug metabolism

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