Solvent-freeze-out (SFO) technology: A controlled crystallization process—Case study of jack bean urease

Yu, Xiaohua; Wang, Jingkang; Ulrich, Joachim

doi:10.1016/j.ces.2015.05.012

Cited by 5 publications

(9 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, a cooling rate of 0.02 K min -1 was utilized based on batch SFO crystallizations. In general, with an increased cooling rate, an increased amount of protein would be trapped in the ice layer, as presented in our previous work [7]. However, a slow cooling rate is time consuming.…”

Section: Purification Of L-asparaginase II Using Sfo Crystallizationmentioning

confidence: 63%

“…The SFO crystallization of L-asparaginase II was investigated on the laboratory scale using the setup used in previous works [6][7][8][9]. The setup consisted of a double-jacketed beaker, a cold finger (a cold tube with closed bottom serving as a cooling surface to induce crystallization on its surface, as with static-layer melt crystallization [7]), and two thermostats. During the SFO crystallization, the temperature of the double-jacketed beaker was kept at 265 K, whereas the cold finger was cooled down to a final temperature of 253 K with a cooling rate of 0.02 K min -1 and was maintained at 253 K for further crystallization.…”

Section: Purification Of L-asparaginase II Using Sfo Crystallizationmentioning

confidence: 99%

“…Hence, the precipitant consumed can be further decreased. Based on a previous investigation [7], the concentration of ethanol was determined to be in the scope of 15 to 25 vol % when the protein concentration was 10 mg mL -1 .…”

Section: Preliminary Determination Of Crystallization Conditionsmentioning

confidence: 99%

“…The amount of precipitant can be effectively reduced. In addition, the protein crystallization can be controlled by the control of ice crystallization proven in previous works [6][7][8][9]. Therefore, the quality of the crystals can be improved.…”

Section: Introductionmentioning

confidence: 99%

“…The feasibility of using SFO technology for protein purification was proven in previous investigations through the purification of urease from its original source, jack bean meal (the content of urease is 0.1 % in jack bean meal) [7]. Urease crystals possessing robust hexagonal morphology were generated from SFO processes with a purity higher than 90 % [7]. Herein, the application of solvent-freeze-out technology was further expanded to recombinant proteins.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Purification of Recombinant L‐Asparaginase II Using Solvent‐Freeze‐Out Technology

Huang

et al. 2018

Chem Eng & Technol

Self Cite

View full text Add to dashboard Cite

The purification of recombinant L‐asparaginase II from an Escherichia coli fermentation broth was carried out by using solvent‐freeze‐out technology on the lab scale. Preliminary crystallization conditions were determined by using batch crystallization screening. The purification of L‐asparaginase II was performed afterwards. Protein crystals with rhombic shape were obtained from the solvent‐freeze‐out crystallization using ethanol. The purity of the crystals was evaluated with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) together with UV spectrophotometry. It was found that DNA could be mostly removed by using solvent‐freeze‐out crystallization. Furthermore, the enzymatic activity was preserved.

show abstract

Section: Purification Of L-asparaginase II Using Sfo Crystallizationmentioning

confidence: 63%

Section: Purification Of L-asparaginase II Using Sfo Crystallizationmentioning

confidence: 99%

Section: Preliminary Determination Of Crystallization Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Purification of Recombinant L‐Asparaginase II Using Solvent‐Freeze‐Out Technology

Huang

et al. 2018

Chem Eng & Technol

Self Cite

View full text Add to dashboard Cite

show abstract

Crystallization and Purification of 4,4′‐Diaminodiphenyl Ether

Chen

Han

et al. 2020

Chem Eng & Technol

View full text Add to dashboard Cite

The purification of 4,4 -diaminodiphenyl ether (ODA) was investigated. ODA, which contained impurities, was sublimated and anti-solvent (ethanol)-crystallized in N-dimethylformamide (DMF). Fourier transform infrared (FTIR) results revealed that the sublimated crystal was basically identical with the pure ODA sample. The influence of different amounts of ethanol on particle size distribution was analyzed during anti-solvent crystallization. The morphology of the ODA crystal was examined by scanning electron microscopy (SEM). The color of the sample enhanced from a brown powder to a colorless granular crystal. The purity of the sample could be increased from about 30 % to 87 %. With the operations of sublimation and anti-solvent crystallization, the purity, crystal morphology, and particle size distribution of ODA were significantly improved.

show abstract

Progressive Freezing Method for Concentrating Tetrahydrofuran (THF) Remained in Grignard Reagent Wastewater

et al. 2019

View full text Add to dashboard Cite

Progressive freezing technology was developed as a promising method to concentrate tetrahydrofuran (THF) remained in Grignard reagent wastewater. Effects of freezing temperature, ice formation rate, and seeding ice on ice purity were characterized. Results indicated the peak removal efficiency of chemical oxygen demand (COD) with the concentration of 3,050 mg⋅L−1 occurred at the lowest ice formation rate of 20% and −4.0°C. The minimal ice electrical conductivity happened at −6.0°C with the ice formation rate of 40%. Seeding ice improved ice purity in the range of −0.5 to −1.0°C. Subsequent test ran in plant revealed the potential application of this approach to concentrate THF from Grignard reagent wastewater. COD removal efficiency peaked at 98.09% at the stirring rate of 200 rpm and the cooling gear of 1 block. Moreover, the theoretical energy consumption was 38.26 kW⋅h per one ton of wastewater.

show abstract

Solvent-freeze-out (SFO) technology: A controlled crystallization process—Case study of jack bean urease

Cited by 5 publications

References 28 publications

Purification of Recombinant L‐Asparaginase II Using Solvent‐Freeze‐Out Technology

Purification of Recombinant L‐Asparaginase II Using Solvent‐Freeze‐Out Technology

Crystallization and Purification of 4,4′‐Diaminodiphenyl Ether

Progressive Freezing Method for Concentrating Tetrahydrofuran (THF) Remained in Grignard Reagent Wastewater

Contact Info

Product

Resources

About