Solvent effects on the retention of oligosaccharides in porous graphitic carbon liquid chromatography

Melmer, Michael; Stangler, Thomas; Premstaller, Andreas; Lindner, Wolfgang

doi:10.1016/j.chroma.2010.07.059

Cited by 25 publications

(18 citation statements)

References 21 publications

(27 reference statements)

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“…The data obtained by this experiment series are qualitatively in accordance with the data from our previous study [20], since enthalpy and entropy values are all positive and increase with the number of glucose units. But quantitative comparison reveals discrepancies, as data with both, reduced and oxidized PGC are significantly lower than data from untreated PGC.…”

Section: Influence Of the Redox State Of Pgc On The Retention Of Maltsupporting

confidence: 90%

“…According to our previous investigations, the gain of entropy correlates with the area of interaction between the analyte and the PGC surface [20]. Hence lower entropy values, as resulting from oxidation of PGC, may indicate diminished interactions between the adsorbed molecules and the stationary phase.…”

Section: Influence Of the Redox State Of Pgc On The Retention Of Maltmentioning

confidence: 94%

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Effects of the redox state of porous graphitic carbon on the retention of oligosaccharides

Melmer

Stangler

Premstaller

et al. 2010

Journal of Chromatography A

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Section: Influence Of the Redox State Of Pgc On The Retention Of Maltsupporting

confidence: 90%

Section: Influence Of the Redox State Of Pgc On The Retention Of Maltmentioning

confidence: 94%

Effects of the redox state of porous graphitic carbon on the retention of oligosaccharides

Melmer

Stangler

Premstaller

et al. 2010

Journal of Chromatography A

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“…The strong adsorption of polar analytes on PGC compared to conventional reversed-phases even allows for the analysis of native and reduced oligosaccharides [13,14]. In previous experiments we identified the nature of the organic modifier, the column temperature and the redox state of the PGC material as important parameters impacting retention of oligosaccharides [15,16].…”

Section: Introductionmentioning

confidence: 97%

Comparison of hydrophilic-interaction, reversed-phase and porous graphitic carbon chromatography for glycan analysis

Melmer

Stangler

Premstaller

et al. 2011

Journal of Chromatography A

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115

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“…It also has a unique retention mechanism, that is, a polar retention effect on graphite that results from charge-induced dipoles on the graphitic surface. [2,26] Because the retention mechanism of PGC is different from that of silica-based materials, PGC can be used as an orthogonal phase to retain and separate polar compounds that elute quickly from reversed phase columns. In our previous papers, we reported core-shell particles for HPLC from carbon cores, an amine containing polymer, and nanodiamond.…”

Section: Introductionmentioning

confidence: 99%

Multi‐instrument characterization of poly(divinylbenzene) microspheres for use in liquid chromatography: as received, air oxidized, carbonized, and acid treated

Hung

Singh

Landowski

et al. 2015

Surface & Interface Analysis

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*We report a multi-instrument characterization of the carbon particles in carbon/polymer/nanodiamond core-shell materials used for high-performance liquid chromatography. These particles are prepared by the carbonization/pyrolysis of poly(divinylbenzene) (PDVB) microspheres. Scanning electron microscopy showed that the particles (4.9 μm initially) decreased in size after air oxidation (to 4.4 μm) and again after carbonization (down to 3.5 μm) but remained highly spherical. Brunauer-Emmett-Teller measurements showed low surface areas initially (as received: 1.6 m 2 /g, after air oxidation: 2.6 m 2 /g) but high values after carbonization (445 m 2 /g). Fourier transform infrared spectroscopy revealed the changes in the functional groups after air oxidation (C = O and C-O stretches appear), carbonization (carbon-oxygen containing moieties disappear), and acid treatment (reintroduction of carbon-oxygen containing moieties). X-ray photoelectron spectroscopy (XPS) and elemental analysis revealed the surface and bulk oxygen contents before and after treatments. By XPS, the atom percent oxygen for the as received, air oxidized, carbonized, and acid treated particles are 8.7, 16.6, 3.7, and 13.8, respectively, and by elemental analysis, the percent oxygen in the materials is 0.6, 8.1, 0.9, 16.9, respectively. A principal components analysis of time-of-flight secondary ion mass spectrometry data identified ions that were enhanced in the different materials, where almost 90% of the variation in the analyzed peak areas was captured by two principle components. X-ray diffraction and Raman spectroscopy suggested that the carbonized PDVB was disordered. Thermogravimetric analysis showed significant differences between the differently treated PDVB microspheres. This work applies directly to a commercial product and the process for preparing it.

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Solvent effects on the retention of oligosaccharides in porous graphitic carbon liquid chromatography

Cited by 25 publications

References 21 publications

Effects of the redox state of porous graphitic carbon on the retention of oligosaccharides

Effects of the redox state of porous graphitic carbon on the retention of oligosaccharides

Comparison of hydrophilic-interaction, reversed-phase and porous graphitic carbon chromatography for glycan analysis

Multi‐instrument characterization of poly(divinylbenzene) microspheres for use in liquid chromatography: as received, air oxidized, carbonized, and acid treated

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