Solvent effects in gel permeation chromatography

Bergmann, John G.; Duffy, Laurence; Stevenson, Reston

doi:10.1021/ac60296a005

Cited by 37 publications

(16 citation statements)

References 4 publications

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“…The effects of solvents on the retentions of alkanes and aromatics with similar molecular volume in HPSEC have been reported by Bergmann and Duffy [23]. The effects were an association between the aromatic and the polystyrene/divinyl benzene gel.…”

Section: Effect Of Other Solvents On the Elution Of Acylglycerol Famsupporting

confidence: 75%

Separation of acylglycerols, FAME and FFA in biodiesel by size exclusion chromatography

Kittirattanapiboon

Krisnangkura

2008

Euro J Lipid Sci & Tech

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Size-exclusion chromatography separates solutes according to their molecular sizes. Free fatty acids (FFA), fatty acid methyl esters (FAME) and monoacylglycerols (MG) of vegetable oils or animal fats have very close molecular sizes and they cannot be baseline-separated on a single Phenogel column (100 Å, 300 mm 6 7.8 mm ID, 5 mm) by using tetrahydrofuran (THF) as the mobile phase. When toluene is used as the mobile phase, triacylglycerols (TG), diacylglycerols (DG), MG and FAME are well separated but there is no baseline resolution between DG and FAME. In addition, the elution order of MG and FAME is reversed. However, baseline separation of all the above lipid classes can be achieved by using toluene containing THF, acetone, dichloromethane, ethyl acetate or acetic acid as the solvent modifier. Acetic acid (0.25%) as the solvent modifier gives the best resolution and all the reference peaks are symmetrical. The detection limit of each class of lipids is 0.1 mg. The correlation coefficient values (between 1 and 100 mg) of all the lipid classes are better than 0.99. Thus, the determination of biodiesel products in the biodiesel reactor is very much simplified.

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Section: Effect Of Other Solvents On the Elution Of Acylglycerol Famsupporting

confidence: 75%

Separation of acylglycerols, FAME and FFA in biodiesel by size exclusion chromatography

Kittirattanapiboon

Krisnangkura

2008

Euro J Lipid Sci & Tech

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“…The study of gel permeation chromatography (GPC) of petroleum resins, complex mixtures of high molecular weight compounds, including homologues of alkanes and aromatic compounds, by eluting low molecular weight model compounds in various solvents and their combinations was treated by American researchers Bergmann and Duffy. 101 The GPC process was mainly carried out in a THF solvent. An abnormal behavior of pericondensed molecules was detected.…”

Section: Adsorption Chromatography Andmentioning

confidence: 99%

Simple Methods for the Separation of Various Subfractions from Coal and Petroleum Asphaltenes

et al. 2020

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This review contains some actual information about more interesting and perspective methods of coal and petroleum asphaltenes separation, fractionation, extraction, adsorption, and complexation. These are the most convenient and simple methods for isolating various components of asphaltenes. These methods are based on the electrostatic and donor–acceptor properties of the used reagents and on unique and incompletely understood phenomena. A promising area is the use of ionic liquids as a convenient and mild isolation of asphaltene components. Understanding and identifying the diverse molecular composition of asphaltene components will help one to understand their nature more fully in the future and, more precisely, physicochemical interactions and phenomena.

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“…Quantitative characterization of pitches and polymeric pitches for molecular weight distribution by gel permeation chromatography has generally been plagued by nonideal elution behavior of the smaller molecular weight constituents (4)(5)(6)(7)(8)(9)(10) and by insufficient solubility of the higher molecular weight constituents (11). The nonideal elution behavior, particularly noticeable for the pericondensed polynuclear aromatics, has been observed in tetrahydrofuran (8), toluene 0003-2700/80/0352-1877801.00/0 (9), benzene (9, 10), and methylene chloride (4, 5). This behavior, sometimes attributed to adsorption (10), generally results in a nonlinear calibration curve at low molecular weights for petroleum residuals (12,13) and polymeric pitches (14).…”

mentioning

confidence: 99%

“…This behavior, sometimes attributed to adsorption (10), generally results in a nonlinear calibration curve at low molecular weights for petroleum residuals (12,13) and polymeric pitches (14). Bergmann et al (9) have reported that the anomalous elution behavior of pitch molecules disappears when 1,2,4trichlorobenzene is used as the gel permeation chromatographic (GPC) solvent. However, interfering negative peaks were observed in 1,2,4-trichlorobenzene for the GPC elution of pitch samples (14).…”

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confidence: 99%

Determination of molecular weight distributions of polymerized petroleum pitch by gel permeation chromatography with quinoline eluent

Greinke¹,

O'Connor²

1980

Anal. Chem.

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A significant advantage peculiar to this industry is that practically all of the radiologically "hot" sample components are separated from the solution that is actually injected into the ion chromatograph. This means that the instrument does not become contaminated with radionuclides to the extent that it would with direct sample injection. This significantly reduces personnel exposure to radiation and in practice saves the trouble and expense of trying to clean up or replace (and correctly dispose of) the "hot" columns.

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Solvent effects in gel permeation chromatography

Cited by 37 publications

References 4 publications

Separation of acylglycerols, FAME and FFA in biodiesel by size exclusion chromatography

Separation of acylglycerols, FAME and FFA in biodiesel by size exclusion chromatography

Simple Methods for the Separation of Various Subfractions from Coal and Petroleum Asphaltenes

Determination of molecular weight distributions of polymerized petroleum pitch by gel permeation chromatography with quinoline eluent

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