Solution NMR Studies of Anesthetic Interactions with Ion Channels

Abstract

NMR spectroscopy is one of the major tools to provide atomic resolution protein structural information. It has been used to elucidate the molecular details of interactions between anesthetics and ion channels, to identify anesthetic binding sites, and to characterize channel dynamics and changes introduced by anesthetics. In this chapter, we present solution NMR methods essential for investigating interactions between ion channels and general anesthetics, including both volatile and intravenous anesthetics. Case studies are provided with a focus on pentameric ligand-gated ion channels and the voltage-gated sodium channel NaChBac.

Reduction in hypoxia‐reoxygenation‐induced myocardial mitochondrial damage with exogenous methane

“…Earlier notions about the molecular mechanism by which CH 4 exerts a non‐specific action were linked to the physical properties of the molecule. Hydrocarbon gases may modulate the structure and function of biological membranes, which has been demonstrated in lipid bilayer models in vitro 27,28 and in animal models in vivo 29,30 . As the smallest hydrocarbon molecule, CH 4 may interact with the cell membrane, leading to haemolysis in erythrocytes in a concentration‐dependent manner 27 .…”

“…Hydrocarbon gases may modulate the structure and function of biological membranes, which has been demonstrated in lipid bilayer models in vitro 27,28 and in animal models in vivo. 29,30 As the smallest hydrocarbon molecule, CH 4 may interact with the cell membrane, leading to haemolysis in erythrocytes in a concentration-dependent manner. 27 In our study, a non-specific action of CH 4 was demonstrated by increased OxPhos capacity and ameliorated leak respiration in the sI/R + CH 4 group, linked to the preserved membrane integrity and electron transfer capacity of mitochondria.…”