Soluble sugars and germination of Annona emarginata (Schltdl.) H. Rainer seeds submitted to immersion in GA3 up to different water contents

Gimenez, Juliana Iassia; Ferreira, Gisela; Corsato, Jaqueline Malagutti

doi:10.1590/s0100-29452014000500033

Cited by 7 publications

(3 citation statements)

References 16 publications

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“…The present study confirms the importance of generating a seed imbibition curve to determine the duration of treatments involving seed immersion in aqueous solutions (Braga et al 2010;Gimenez et al 2014a;b). It is also important to understand the metabolic processes occurring at different seed water contents until the end of imbibition to avoid wrongfully interpreting the results.…”

Section: Discussionsupporting

confidence: 81%

Slow imbibition of Annona emarginata (Annonaceae) seeds: metabolic and ultrastructural evaluations

Gimenez

Amaro

Machado

et al. 2017

Botany

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Germination pattern may be affected when seeds are dispersed under flooded conditions or subjected to pre-germination treatments such as immersion in plant growth regulators and priming. We evaluated the metabolic and ultrastructural changes in the seeds of Annona emarginata (Schltdl.) H. Rainer seeds caused by immersion in water (0 MPa) during the imbibition phase (phase I). The immersion time necessary for initially dry seeds (10% water content) to reach a water content of 15%, 20%, and 35% was recorded. The seeds were also immersed in a polyethylene glycol 6000 solution (–1.2 MPa) to decrease the rate of water uptake and increase the immersion times required for the seeds to reach the selected water contents. With shorter immersion times, seed damage did not decrease germinability. Seeds immersed for 122 h sustained damage to the cell membrane system and organelles, resulting in a decreased percentage and rate of germination. Decreasing the rate of water uptake increased the time required to finish imbibition phase to 317 h and resulted in more ultrastructural damages and a decreased germination index. We conclude that in seeds with slower imbibition, longer immersion times may cause severe metabolic damage if the seeds are immersed until the end of the imbibition phase, resulting in reduced germination.

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Section: Discussionsupporting

confidence: 81%

Slow imbibition of Annona emarginata (Annonaceae) seeds: metabolic and ultrastructural evaluations

Gimenez

Amaro

Machado

et al. 2017

Botany

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“…As proposed by [38], seed water intake is triphasic in nature and can be divided into phases I, II, and III. The triphasic pattern of seed water uptake observed in this study has been reported by many authors, for example, Nicotiana tabacum [39], Zea mays [40], and Annona emarginata [41]. In priming, determination of seed imbibition curves helped in setting the times of invigoration treatments, which were 18 h (B. speciosus), 20 h (Co. erythrophyllum), 20 h (E. caffra), 24 h (P. sativum), and 24 h (Cu.…”

Section: Seed Water Imbibitionsupporting

confidence: 88%

A Comparison of Water Imbibition and Controlled Deterioration in Five Orthodox Species

2022

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Orthodox seeds deteriorate even when stored in the best of conditions; hence, it is very important to monitor germination in stored seeds. To assess orthodox seed deterioration, a germination test is usually employed. This study assessed and compared seed deterioration in five orthodox species using electrolyte leakage and Fourier transform infrared spectroscopy (FTIR). The study also compared water imbibition by the test orthodox seeds. To achieve this, the seeds from three wild (Bolusanthus speciosus; Combretum erythrophyllum; Erythrina caffra) and two agricultural (Pisum sativum and Cucurbita pepo) species were imbibed between 20 layers of single-ply paper towel. The other set was subjected to controlled deterioration at 40 °C and 100% relative humidity for 32 d, with samples taken for germination and electrolyte leakage measurement at 4 d intervals. FTIR measurements were performed at 0, 20, and 32 d of controlled deterioration. The results indicated that there were some significant interspecies differences in the imbibition times and seed water contents but these were not large. In all species, uptake of water was complete between about 15 and 25 h. The wild species showed higher sensitivity to controlled deterioration. Complete loss of germinability occurred much earlier in the wild species (20 d in B. speciosus and E. caffra, and 16 d in Co. erythrophyllum) compared with 36 d for the agricultural species P. sativum and Cu. pepo. There was a negative correlation between electrolyte leakage and seed germination in all wild and agricultural species. A strong positive correlation was observed between the time of controlled deterioration, electrolyte leakage, and FTIR transmission in all the species. While controlled deterioration may help in decisions relating to the storage of orthodox seeds, the water imbibition results from this study will help to set the priming time of the species. The study reaffirms electrolyte leakage as an indicator of seed viability in P. sativum and Cu. pepo; it also recommends the use of electrolyte leakage as an indicator of seed deterioration in B. speciosus, Co. erythrophyllum, and E. caffra. The study also recommends FTIR as a tool for monitoring the germination of stored seeds of all the test species.

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“…Therefore, germination is slow and uneven . In this context, research has been conducted over time with the use of plant growth regulators in order to promote germination in species of the family Annonaceae used as crown or rootstock Gimenez et al, 2014). For atemoya seeds, Braga et al (2010) obtained the highest germination percentage (95.4%) using a concentration of 329 mg.L -1 of GA 4+7 + N-(phenylmethyl)-aminopurine.…”

Section: Introductionmentioning

confidence: 99%

Tetrazolium test for assessment of seed viability of atemoya (Annona cherimola Mill. x A. squamosa L.)

2014

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-The study aimed to adapt the tetrazolium test to assess the viability of atemoya (Annona cherimola Mill. x A. squamosa L.) seeds. The experimental design was completely randomized in 3 x 3 factorial arrangement (tetrazolium salt concentrations x exposure times), totaling 9 treatments with four replicates of 25 seeds. After immersion in water for 24 hours, the seeds were longitudinally sectioned and exposed to three tetrazolium salt concentrations (0.05%, 0.5% and 1%) for three periods of time (2, 4 and 6 hours). The evaluations were performed according to pre-established criteria of tissue integrity and staining. The germination test was performed, using the GA 4+7 + N-(fenilmetil)-aminopurina regulators at concentrations of 0 and 300 mg.L -1 , because dormancy is observed in species of the family Annonaceae. Tetrazolium salt at 1% yielded the highest percentage of viable seeds within the shortest time, while longer exposure times were necessary with concentrations of 0.05% and 0.5%. It can be concluded that the concentration of 1% of tetrazolium salt for two hours of exposure is more appropriate for assessing the viability of atemoya seeds.

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Soluble sugars and germination of Annona emarginata (Schltdl.) H. Rainer seeds submitted to immersion in GA3 up to different water contents

Cited by 7 publications

References 16 publications

Slow imbibition of Annona emarginata (Annonaceae) seeds: metabolic and ultrastructural evaluations

Slow imbibition of Annona emarginata (Annonaceae) seeds: metabolic and ultrastructural evaluations

A Comparison of Water Imbibition and Controlled Deterioration in Five Orthodox Species

Tetrazolium test for assessment of seed viability of atemoya (Annona cherimola Mill. x A. squamosa L.)

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