Soluble L-selectin levels in serum and cerebrospinal fluid in patients with multiple sclerosis and systemic lupus erythematosus

Baraczka, Krisztina; Pozsonyi, T; Nékám, K; Viranyi, Marian; Seszták, Magdolna; Szongoth, M; Jakab, László

doi:10.1034/j.1600-0404.2000.102002114.x

Cited by 13 publications

(5 citation statements)

References 8 publications

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“…The increased concentration of L-selectin found in CSF in our study corresponds to other findings reported in patients with non-infectious inflammation of CNS, i.e. MS and systemic lupus erythematosus [24]. As reported previously, L-selectin is rapidly shed from the cell surface, a process that appears to be important for limiting leucocyte accumulation at the sites of inflammation [25].…”

Section: Discussionsupporting

confidence: 90%

Increase in Adhesion Molecules in Cerebrospinal Fluid of Children with Mumps and Mumps Meningitis

Sulik¹,

Wojtkowska

Rożkiewicz³

et al. 2006

Scand J Immunol

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Adhesion molecules play a key role in leucocyte migration into the central nervous system (CNS). Concentrations of endothelial‐derived soluble intercellular adhesion molecule‐1 (sICAM‐1) and leucocyte‐originated soluble L‐selectin (sL‐selectin) in cerebrospinal fluid (CSF) of children with mumps meningitis (mononuclear pleocytosis, n = 33) and mumps (absence of pleocytosis, n = 9) were compared with values from age‐matched control group (n = 19). In 14 patients from the meningitis group, adhesion molecule levels together with albumin concentration were estimated in paired CSF/serum samples to calculate concentration quotients and determine molecule intrathecal release. Both sICAM‐1 (median 3.44 versus 0.86 ng/ml; P < 0.0001) and sL‐selectin (median 29.91 versus 8.52 ng/ml; P < 0.0001) concentrations in CSF were increased in mumps meningitis patients compared with controls. Increased levels of the selected adhesion molecules were also observed in mumps patients without CNS involvement when compared with controls (median sICAM‐1: 1.14 versus 0.86 ng/ml, sL‐selectin: 13.54 versus 8.52 ng/ml; P < 0.01). Additionally, the concentration of adhesion molecules was found to correlate with CSF leucocyte count. Considerable correlation of sICAM‐1 and sL‐selectin quotients and corresponding albumin quotients suggests that a majority of the soluble adhesion molecules originated from the bloodstream. Analysis of adhesion molecule levels demonstrated indirect evidence of brain‐derived fractions. Our results suggest the involvement of adhesion molecules during the early phase of mumps meningitis.

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Section: Discussionsupporting

confidence: 90%

Increase in Adhesion Molecules in Cerebrospinal Fluid of Children with Mumps and Mumps Meningitis

Sulik¹,

Wojtkowska

Rożkiewicz³

et al. 2006

Scand J Immunol

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“…L-selectin (CD62L) is a member of the selectin family of adhesion molecules expressed in leukocytes ( 52 ). Soluble L-selectin (sL-selectin) is elevated in the serum ( 53 ) and cerebrospinal fluid ( 54 ) of patients in comparison to healthy donors. Levels of sL-selectin correlate significantly with the levels of antibodies to dsDNA in patients with SLE ( 55 ).…”

Section: Discussionmentioning

confidence: 99%

Combined proteomics and single cell RNA-sequencing analysis to identify biomarkers of disease diagnosis and disease exacerbation for systemic lupus erythematosus

Liao

et al. 2022

Front. Immunol.

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IntroductionSystemic lupus erythematosus (SLE) is a chronic autoimmune disease for which there is no cure. Effective diagnosis and precise assessment of disease exacerbation remains a major challenge.MethodsWe performed peripheral blood mononuclear cell (PBMC) proteomics of a discovery cohort, including patients with active SLE and inactive SLE, patients with rheumatoid arthritis (RA), and healthy controls (HC). Then, we performed a machine learning pipeline to identify biomarker combinations. The biomarker combinations were further validated using enzyme-linked immunosorbent assays (ELISAs) in another cohort. Single-cell RNA sequencing (scRNA-seq) data from active SLE, inactive SLE, and HC PBMC samples further elucidated the potential immune cellular sources of each of these PBMC biomarkers.ResultsScreening of the PBMC proteome identified 1023, 168, and 124 proteins that were significantly different between SLE vs. HC, SLE vs. RA, and active SLE vs. inactive SLE, respectively. The machine learning pipeline identified two biomarker combinations that accurately distinguished patients with SLE from controls and discriminated between active and inactive SLE. The validated results of ELISAs for two biomarker combinations were in line with the discovery cohort results. Among them, the six-protein combination (IFIT3, MX1, TOMM40, STAT1, STAT2, and OAS3) exhibited good performance for SLE disease diagnosis, with AUC of 0.723 and 0.815 for distinguishing SLE from HC and RA, respectively. Nine-protein combination (PHACTR2, GOT2, L-selectin, CMC4, MAP2K1, CMPK2, ECPAS, SRA1, and STAT2) showed a robust performance in assessing disease exacerbation (AUC=0.990). Further, the potential immune cellular sources of nine PBMC biomarkers, which had the consistent changes with the proteomics data, were elucidated by PBMC scRNAseq.DiscussionUnbiased proteomic quantification and experimental validation of PBMC samples from two cohorts of patients with SLE were identified as biomarker combinations for diagnosis and activity monitoring. Furthermore, the immune cell subtype origin of the biomarkers in the transcript expression level was determined using PBMC scRNAseq. These findings present valuable PBMC biomarkers associated with SLE and may reveal potential therapeutic targets.

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“…multiple scoliosis (MS) and systemic lupus erythematosus. [24] As reported previously, L-selectin is rapidly shed from the cell surface, a process that appears to be important for limiting leukocyte accumulation at the sites of inflammation. [25] This shedding is accomplished by the cleavage of cell surface L-selectin which results in an accumulation of the soluble form of the adhesion molecule.…”

Section: Discussionmentioning

confidence: 80%

Adhesion molecule levels in serum and cerebrospinal fluid in children with bacterial meningitis and sepsis

Jaber

Hamed

2009

J Pediatr Neurosci

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Background:Adhesion molecules play a role in leukocyte recruitment during central nervous system (CNS) inflammation.Aim:This study was designed to compare serum, cerebrospinal fluid (CSF) concentrations of adhesion molecules in children with meningitis and sepsis, and to evaluate their sources.Setting:This study was carried out at Pediatric Department, King Abdulaziz University Hospital from January 2007 to June 2008.Design:Serum and CSF samples were collected on admission from meningitis (n = 40), sepsis (n = 20) patients, and sera from controls (n = 20).Materials and Methods:Endothelial (E), leukocyte (L), platelet (P) selectins intercellular cell adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecules-1 (VCAM-1) were measured using ELISA.Statistics:ANOVA and Spearman's correlations were used. Adhesion molecules with albumin concentration were estimated in CSF/serum to calculate concentration quotients.Results:In meningitis, serum sE-, sL-, sP-selectins sICAM-1, sVCAM-1 levels were higher than controls. Compared to sepsis, serum sE-selectin, sL-selectin, sVCAM-1, CSF-sL-selectin, CSF-sVCAM-1, VCAM-1 ratio and index were higher, while serum sP-selectin was lower than meningitis. sE-selectin ratio, CSF sICAM-1 were higher in meningitis with positive than negative culture. The sE-selectin index was higher in meningitis with neurological complication than those without it. In meningitis, correlation was found between CSF protein and CSF white blood cell counts (WBCs), CSF sICAM-1, CSF sVCAM-1 and between CSF sE-selectin and CSF sICAM-1.Conclusions:This study supports the role of adhesion molecules especially sL-selectin, sVCAM-1 in meningitis and suggests further research to determine their use as biomarkers for meningitis and use of their antagonists as therapeutic for CNS inflammation. The presence of discrepancy of CSF/serum ratios for molecules of same molecular weight suggest intrathecal shedding in addition to diffusion through the blood-CSF barrier.

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Soluble L-selectin levels in serum and cerebrospinal fluid in patients with multiple sclerosis and systemic lupus erythematosus

Cited by 13 publications

References 8 publications

Increase in Adhesion Molecules in Cerebrospinal Fluid of Children with Mumps and Mumps Meningitis

Increase in Adhesion Molecules in Cerebrospinal Fluid of Children with Mumps and Mumps Meningitis

Combined proteomics and single cell RNA-sequencing analysis to identify biomarkers of disease diagnosis and disease exacerbation for systemic lupus erythematosus

Adhesion molecule levels in serum and cerebrospinal fluid in children with bacterial meningitis and sepsis

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