Soluble Hla Class Ii Concentrations in Normal Individuals and Transplant Recipients

Soluble HLA measurement in saliva and cerebrospinal fluid in Caucasian patients with multiple sclerosis: a preliminary study

“…It has been reported that the presence of soluble HLA can be explained, at least in part, by the shedding of cell bound HLA [8]. We have observed no correlation between sHLA-I and sHLA-II levels in the sera of normal individuals [9]. sHLA-I was either non-detectable, or present in very low quantities, in the urine, sweat, saliva and tears of normal individuals.…”

“…The levels of sHLA-II were determined using a previously described assay [9,11] with minor modifications. Briefly, test samples were added to appropriate wells containing an anti-Class I (W6/32) or anti-Class II (Ab2.06) monoclonal antibody (Mab) coated beads.…”

“…For sHLA-II values, the wide range of sHLA-II concentrations reported from studies of serum of normal individuals [3,9,17,26-30] seem likely to reflect the use of various standards or to the different characteristics of the monoclonal antibodies used in described techniques. Although initial standardization of the sHLA-II assay has been made previously and reported by us [9], in this study, for greater precision of analysis, the amount of sHLA-II was inferred directly from the ELISA absorbance value (OD) within each sample of body fluid tested.…”

“…Although initial standardization of the sHLA-II assay has been made previously and reported by us [9], in this study, for greater precision of analysis, the amount of sHLA-II was inferred directly from the ELISA absorbance value (OD) within each sample of body fluid tested. The OD of studied samples corresponding to sHLA-II values were compared with the OD values of 5% BSA that had been utilized as dilution factor and negative control within each procedure.…”

Saliva soluble HLA as a potential marker of response to interferon-β1a in multiple sclerosis: A preliminary study

“…Normal individuals have stable concentrations of sHLA-I and sHLA-II in their serum [1]. However, the serum level of sHLA-I is significantly elevated in patients with various inflammatory diseases [5-8] although this is not necessarily the case for serum sHLA-II levels [9,10]. Preliminary evidence suggests that patients with systemic lupus erythematosus (SLE) are at increased risk of developing active disease in the presence of high sHLA-I levels in the saliva, while sHLA-II level has not been observed to be elevated in rheumatological diseases [11].…”