Soluble Fragments of E-Cadherin Cell-Adhesion Molecule Increase in Urinary-Excretion of Cancer-Patients, Potentially Indicating Its Shedding From Epithelial Tumor-Cells

Katayama, Masahiro; Hirai, Syu-ichi; Yasumoto, Makiko; Nishikawa, K; Nagata, Shinji; Kamihagi, K; Kato, Ichiroh

doi:10.3892/ijo.5.5.1049

Cited by 15 publications

(16 citation statements)

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“…Also, the loss of E-cadherin function is a causal factor in the promotion of invasion and metastasis. 33 High levels of soluble E-cadherin in serum 34 and urine 35 of patients with cancer and coexpression of MMPs and E-cadherin cell metastasis studies 36 support that statement. Based on animal studies, and the role of MMPs and E-cadherin in modulation mechanism of cancer invasion, we hypothesized that MMP-2, MMP-9, and E-cadherin can be expressed in the endometrium during the implantation window and are involved in the implantation process in women.…”

Section: Introductionmentioning

confidence: 93%

Matrix Metalloproteinases 2 and 9 and E-Cadherin Expression in the Endometrium During the Implantation Window of Infertile Women Before In Vitro Fertilization Treatment

Maia-Filho

Rocha²,

Ferreira

et al. 2015

Reprod. Sci.

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Objective: To evaluate the expression of endometrial matrix metalloproteinases (MMPs) 2 and 9 and E-cadherin in periimplantation phase of infertile women who have undergone in vitro fertilization (IVF) cycles. Methods: This prospective study included 51 patients who underwent endometrial biopsy during the receptive phase in a menstrual cycle prior to IVF treatment. The samples were evaluated by tissue microarray for immunohistochemical study. Results: The expression of MMP-2, MMP-9, and E-cadherin in the endometrium prior to IVF treatment was not associated with pregnancy. There was a decrease in E-cadherin immunodetection, the higher the age of the patients, a negative relationship between E-cadherin and MMP-2, and a positive association between MMP-9 and E-cadherin. Conclusions: The MMP-2, MMP-9, and E-cadherin are expressed in the endometrium of infertile patients during the receptive phase of the natural menstrual cycle. However, there is no correlation between the expression of these molecules and the clinical IVF outcomes.

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Section: Introductionmentioning

confidence: 93%

Matrix Metalloproteinases 2 and 9 and E-Cadherin Expression in the Endometrium During the Implantation Window of Infertile Women Before In Vitro Fertilization Treatment

Maia-Filho

Rocha²,

Ferreira

et al. 2015

Reprod. Sci.

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“…In patients with cancer, high levels of circulating ICAM-1 have been found to be associated with the presence of liver metastases in gastric, colonic, gall bladder and pancreatic cancer (Tsujisaki et al, 1991), with poor survival of patients with malignant melanoma (Harning et al, 1991;Altomonte et al, 1992;Kageshita et al, 1992Kageshita et al, , 1993Viac et al, 1993) and correlated with disease stage and progression in Hodgkin's disease (Gruss et al, 1993). Soluble forms of E-cadherin have been identified in serum and urine of normal individuals and patients with cancer and cutaneous diseases (Katayama et al, 1994b;Banks et al, 1995;Matsuyoshi et al, 1995).…”

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confidence: 99%

Circulating soluble adhesion molecules E-cadherin, E-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in patients with gastric cancer

Velikova

Banks²,

Gearing³

et al. 1997

Br J Cancer

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Summary The concentrations of the soluble adhesion molecules E-cadherin, E-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were investigated in 45 patients with gastric cancer before treatment and their correlation with clinical, histological and routine laboratory parameters was examined. Data were collected on tumour stage at presentation, presence and sites of metastatic disease, tumour pathology, survival and results of routine laboratory tests. Serum concentrations of ICAM-1 and VCAM-1 were significantly elevated in the patients with gastric cancer in comparison with the group of healthy subjects (P < 0.00001 and P < 0.0001 respectively). Increased serum concentrations of VCAM-1 were associated with locally advanced and metastatic disease whereas ICAM-1 was significantly elevated both in local and in advanced/metastatic disease. Soluble E-cadherin and E-selectin concentrations did not show any significant elevation in gastric cancer patients. Concentrations of soluble adhesion molecules showed significant correlation with each other (except E-selectin and VCAM-1) and with alkaline phosphatase. Soluble ICAM-1 and VCAM-1 were significantly associated with an elevated total white cell count. Patients with elevated VCAM-1 had significantly poorer survival in comparison with patients with normal serum levels (P= 0.0361).

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“…The technique for in situ visualization of DNA fragmentation was carried out according to the method described by Mori et al (16), which was a modification of the one developed by Gavrieli et al (17) -free insulin, transferrin, and selenium/Dulbecco's modified Eagle's medium, which was supplemented with high (1 mM) or low (0.09 mM) levels of divalent cations by addition of CaCl 2 and MgCl 2 at the indicated concentrations. To study the effect of blocking antibody to E-cadherin, HSC-3 cells were seeded at 6 ϫ 10 5 cells/plate in the presence of mouse anti-human E-cadherin mAb (clone SHE78-7, Zymed Laboratories) at 1 g/ml and incubated for up to 72 h (18,19). Mouse IgG added to monolayer, single cell, and MCA cultures at 1 g/ml was used as control.…”

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confidence: 99%

E-cadherin Regulates Anchorage-independent Growth and Survival in Oral Squamous Cell Carcinoma Cells

Kantak¹,

Kramer²

1998

Journal of Biological Chemistry

210

182

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Integrin-basement membrane interactions provide essential signals that promote survival and growth of epithelial cells, whereas loss of such adhesions triggers programmed cell death. We found that HSC-3 human squamous carcinoma cells survived and grew readily as monolayers, but when they were suspended as single cells, they ceased proliferating and entered into the apoptotic death pathway, characterized by DNA fragmentation. In contrast, if the suspended carcinoma cells were permitted to form E-cadherin-mediated multicellular aggregates, they not only survived but proliferated. However, aggregated normal keratinocytes were unable to survive in suspension culture and rapidly became apoptotic. Anchorage independence and resistance to apoptosis of HSC-3 cell aggregates required high levels of extracellular Ca 2؉ and was inhibited with function-perturbing anti-E-cadherin antibody. Resistance to suspension-induced apoptosis in cell aggregates paralleled the up-regulation of Bcl-2 but occurred in the absence of focal adhesion kinase activation. Analysis of suspension-induced death in a set of cloned squamous epithelial cell lines with different levels of E-cadherin expression revealed that receptor-positive cell clones evaded apoptosis and proliferated in three-dimensional aggregate culture, whereas cadherin-negative clones failed to survive. Collectively, these observations indicate that cadherin-mediated intercellular adhesions generate a compensatory mechanism that promotes anchorage-independent growth and suppresses apoptosis.

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Soluble Fragments of E-Cadherin Cell-Adhesion Molecule Increase in Urinary-Excretion of Cancer-Patients, Potentially Indicating Its Shedding From Epithelial Tumor-Cells

Cited by 15 publications

References 0 publications

Matrix Metalloproteinases 2 and 9 and E-Cadherin Expression in the Endometrium During the Implantation Window of Infertile Women Before In Vitro Fertilization Treatment

Matrix Metalloproteinases 2 and 9 and E-Cadherin Expression in the Endometrium During the Implantation Window of Infertile Women Before In Vitro Fertilization Treatment

Circulating soluble adhesion molecules E-cadherin, E-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in patients with gastric cancer

E-cadherin Regulates Anchorage-independent Growth and Survival in Oral Squamous Cell Carcinoma Cells

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