Soluble fetoproteins in chorioallantoic membrane from chick embryo (Gallus domesticus)

Rodriguez-Burgos, A

doi:10.1016/0305-0491(90)90377-6

Cited by 3 publications

(1 citation statement)

References 4 publications

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“…Previous experiments revealed the presence of seven transitory antigens (TrAg) in allantoic fluid, four of which were also encountered in chick embryo liver [16], and of three TrAg in the chorioallantoic membrane of 13-day chicks [17], in addition to α-fetoprotein in both cases. This aroused the author's interest in searching for antigens in the early embryo, which might be present prior to organogenesis.…”

Section: Introductionmentioning

confidence: 99%

Detection in chick embryo of fetoproteins not recognized by the dam's immune system and of soluble alloantigens. Presumptive teratogenic and abortogenic capacity of their specific IgY

Rodriguez-Burgos

2003

BMC Immunol

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BackgroundThe aim of this work was to detect antigens, non-self to the dam, potentially present in chick embryo prior to organogenesis with a view to establishing the consequences of their neutralization on chick development. To this end, hens were immunized with the extract from embryos incubated for 53 h. Their eggs were either used to isolate immunoglobulins for dot and blot tests or incubated for variable lengths of time.ResultsImmunoblot tests, using adsorbed primary and secondary antibodies against paternal serum, revealed the presence of at least four antigens of 32, 34, 70 and 200 kDa that can be classified as soluble alloantigens. The same antibodies against chick embryo extracts (between 53 h and 9) showed at least five aged antigens of 34, 52, 90, 200 and 250 kDa, not detected in cock serum, that can thus be considered as soluble, foreign to the immunized hens and transitory antigens. The abnormalities observed included arrested development and fetal death, as well as minor functional damage in the few chicks that were born alive. The ratio of abnormal to normal embryos was 2.85 in the experimental group and 0.43 in the control group. With regard to congenital anomalies it must be said that of the 81 eggs incubated only four chicks were born alive, and of these, only one had a healthy birth and subsequent growth. The other three showed a transitory ataxia and one of them presented adult lumbar scoliosis and asymmetric pelvis.ConclusionsThe problem of recurrent spontaneous abortions is revisited in the light of these results. Some recent data suggest that soluble alloantigens may be candidates for a new etiological entity in recurrent spontaneous abortions. They can also be the cause of some congenital anomalies. The soluble, foreign, transitory antigens may have a similar effect although there is no supportive data in the literature.

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Section: Introductionmentioning

confidence: 99%

Detection in chick embryo of fetoproteins not recognized by the dam's immune system and of soluble alloantigens. Presumptive teratogenic and abortogenic capacity of their specific IgY

Rodriguez-Burgos

2003

BMC Immunol

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New fetoproteins, as presumptive antigenic inducers of abortions and congenital anomalies. Temporal forking of immunological abortions. A working hypothesis

Rodriguez-Burgos¹

2004

Medical Hypotheses

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Fatty Acids and Squalene Carried by Alpha Fetoprotein, and Fetal and Adult Serum Albumin from Chicken. Comparison with These from Mammals

1999

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Alpha fetoprotein (C-AFP), serum albumin (C-fSA) from chicken embryos, and SA from hens were purified using gentle chromatographic and electrophoretic methods, and their fatty acids (FAs) and squalene contents were analyzed using gas chromatography and mass spectrometry. In 7-day-old chick embryo, AFP carries 16% and fSA 6% free FAs, the rest being carried as phospholipids, which differs from rat and pig AFP, where all fatty acids are carried like free fatty acids. At this stage C-AFP contains 3.6% arachidonic acid, which falls to 1.7% in 14-day-old embryos. Both of these figures are significantly lower than in humans, rats, calves, and pigs. C-AFP does not transport docosahexaenoic acid, in notable contrast to the mammals mentioned above. The finding of squalene in the two fetal proteins is reported for the first time. During the interval between 7 and 14 days, the proportion of C16:1 n-7 and of C18:2 n-6 increases 10-fold, that of C18:0 quadruples, and that of C18:1 n-9 decreases 3-fold. Squalene increases in this period from 2.2% to 10.0%. The C-fSA of a 7-day-old embryo transports only one FA with more than two unsaturated carbons, arachidonic acid (2.4%). It also contains squalene (1.2%). Similarly, only arachidonic acid (2.5%), but not squalene is found in hen SA. The percentage of saturated and monounsaturated FAs divided by the percentage of polyunsaturated FAs, and the ratios of the percentage of FAs with C14-C18 with respect to those with C20-C22 transported by C-AFP are very different from those of studied mammals.

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Soluble fetoproteins in chorioallantoic membrane from chick embryo (Gallus domesticus)

Cited by 3 publications

References 4 publications

Detection in chick embryo of fetoproteins not recognized by the dam's immune system and of soluble alloantigens. Presumptive teratogenic and abortogenic capacity of their specific IgY

Detection in chick embryo of fetoproteins not recognized by the dam's immune system and of soluble alloantigens. Presumptive teratogenic and abortogenic capacity of their specific IgY

New fetoproteins, as presumptive antigenic inducers of abortions and congenital anomalies. Temporal forking of immunological abortions. A working hypothesis

Fatty Acids and Squalene Carried by Alpha Fetoprotein, and Fetal and Adult Serum Albumin from Chicken. Comparison with These from Mammals

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