Soluble and membrane symbiosis‐related polypeptides associated with the development of arbuscular mycorrhizas in tomato (<i>Lycopersicon esculentum</i>)

Benabdellah, Karim; Azcón, R.; Ferrol, Nuria

doi:10.1046/j.1469-8137.1998.00255.x

Cited by 28 publications

(17 citation statements)

References 28 publications

(24 reference statements)

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“…Regarding the proteomic level, there are a number of reports documenting differential protein patterns in mycorrhizal and non-mycorrhizal roots (Pacovsky 1989;Schellenbaum et al 1992;Arines et al 1993;GarciaGarrido et al 1993;Dumas-Gaudot et al 1994;Simoneau et al 1994;Samra et al 1997;Benabdellah et al 1998;Dassi et al 1999;Fester et al 2002;Repetto et al 2003). Using specific antibodies, Wyss et al (1990) identified some of the induced proteins as nodulins.…”

Section: Model Plants In Am Researchmentioning

confidence: 94%

Molecular and cell biology of arbuscular mycorrhizal symbiosis

Hause

Fester

2004

Planta

144

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The roots of most extant plants are able to become engaged in an interaction with a small group of fungi of the fungal order Glomales (Glomeromycota). This interaction-arbuscular mycorrhizal (AM) symbiosis-is the evolutionary precursor of most other mutualistic root-microbe associations. The molecular analysis of this interaction can elucidate basic principles regarding such associations. This review summarizes our present knowledge about cellular and molecular aspects of AM. Emphasis is placed on morphological changes in colonized cells, transfer of nutrients between both interacting partners, and plant defence responses. Similarities to and differences from other associations of plant and microorganisms are highlighted regarding defence reactions and signal perception.

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Section: Model Plants In Am Researchmentioning

confidence: 94%

Molecular and cell biology of arbuscular mycorrhizal symbiosis

Hause

Fester

2004

Planta

144

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“…In view of the AM symbiosis-highly specific membrane-related features, sub-cellular proteomics targeting the root microsomal fraction has been suggested to be more relevant to address core functional proteins in mycorrhiza. Alterations in the root membrane polypeptide patterns during the development of arbuscular mycorrhiza were first reported in tomato (Benabdellah et al, 1998(Benabdellah et al, , 2000. However, only one protein, corresponding to a down-regulated vacuolar plant H + -ATPase subunit, could be identified following N-terminal sequencing (Benabdellah et al, 2000).…”

Section: Introductionmentioning

confidence: 98%

Identification of Membrane-Associated Proteins Regulated by the Arbuscular Mycorrhizal Symbiosis

et al. 2005

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A sub-cellular proteomic approach was carried out to monitor membrane-associated protein modifications in response to the arbuscular mycorrhizal (AM) symbiosis. Membrane proteins were extracted from Medicago truncatula roots either inoculated or not with the AM fungus Glomus intraradices. Comparative two-dimensional electrophoresis revealed that 36 spots were differentially displayed in response to the fungal colonization including 15 proteins induced, 3 up-regulated and 18 down-regulated. Among them, seven proteins were found to be commonly down-regulated in AM-colonized and phosphate-fertilized roots. Twenty-five spots out of the 36 of interest could be identified by matrix assisted laser desorption/ionisation-time of flight and/or tandem mass spectrometry analyses. Excepting an acid phosphatase and a lectin, none of them was previously reported as being regulated during AM symbiosis. In addition, this proteomic approach allowed us for the first time to identify AM fungal proteins in planta.

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“…In the first of these studies microsomal proteins of tomato roots colonized by G. mosseae were analyzed and a number of spots were shown be responsive to AM mycorrhization [79].…”

Section: Membrane Proteins and Arbuscular Mycorrhizal Interactionsmentioning

confidence: 99%

Proteomic studies of arbuscular mycorrhizal associations

Couto¹,

Lovato²,

Wipf³

et al. 2013

ABC

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Arbuscular mycorrhizal (AM) fungi are soil-borne microorganisms forming mutualistic associations with the vast majority of land plants, including most agricultural relevant crops. In this association the plant provides the fungus with plant photosynthates allowing it to complete its life cycle, while the fungus provides the plant with mineral nutrients, mainly phosphorus and can also help the plant to tolerate biotic and abiotic stresses. In regard to these benefits there is growing interest on the use of AM fungi to improve productivity and sustainability in agricultural systems. AM fungi and their interactions with plants have been extensively studied using proteomic techniques, but some difficulties have been faced. 1) Little is known about the AM fungal typical protein repertoire because it is currently impossible to grow AM fungi in pure axenic cultures; 2) Plant tissues often contain high amounts of interfering substances that make protein extraction for the study of AM interactions a difficult procedure; 3) Most nutrient exchanges between AM fungi and their host plants involve participation of membrane proteins, still poorly resolved in most separation techniques. Finally, 4) the formation of the arbuscule is an asynchronous process, making it difficult to distinguish which proteins are essential in the early or late stages of AM associations. In this review we present a historical summary of how these difficulties have been overcome by technological advances in proteomics and we discuss current and future trends in the study of the proteins involved in AM interactions.

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Soluble and membrane symbiosis‐related polypeptides associated with the development of arbuscular mycorrhizas in tomato (Lycopersicon esculentum)

Cited by 28 publications

References 28 publications

Molecular and cell biology of arbuscular mycorrhizal symbiosis

Molecular and cell biology of arbuscular mycorrhizal symbiosis

Identification of Membrane-Associated Proteins Regulated by the Arbuscular Mycorrhizal Symbiosis

Proteomic studies of arbuscular mycorrhizal associations

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