Solubilização Da Cumarina Em Meio Aquoso E Determinação Da Sua Atividade Antibacteriana

Abstract

A pesquisa teve como objetivo estabelecer o menor grau etanólico para a solubilização da cumarina em meio aquoso e avaliar a sua atividade antibacteriana. Avaliou-se a solubilidade em água destilada e soluções etanólicas 10%, 30%, 50% e 70%, sendo estabelecida a solução etanólica a 30%, como solvente. As concentrações 1. 024, 512, 256, 128, 64, 32, 16, 8, 4 e 2µg/mL (p/v) Maria, v. 42, n.2, p. 195-201, jul./dez. 2016 SOLUBILIZAÇÃO DA CUMARINA EM MEIO AQUOSO E DETERMINAÇÃO DA SUA ATIVIDADE ANTIBACTERIANA COUMARIN SOLUBILIZATION IN AQUEOUS MEDIUM AND DETERMINATION OF THE ITS ACTIVITY ANTIBACTERIAL ABSTRACTThe research aimed to establish the lowest degree ethanolic to solubilize the coumarin in aqueous and to evaluate its antibacterial activity. It was evaluated the solubility in distilled water and solutions ethanolic 10%, 30%, 50% and 70%, it is set at 30% ethanolic solution as a solvent.. The concentrations of 1.024, 512, 256, 128, 64, 32, 16, 8, 4, and 2µg/mL (w/v) of coumarin were tested by the microdilution method against bacteria Staphylococcus aureus (ATCC 14458), Staphylococcus epidermidis (ATCC 12228), Bacillus cereus (ATCC 11778), Listeria monocytogenes (ATCC 7644), Escherichia coli (ATCC 11229), Salmonella enterica subsp. enterica sorovar. Typhimurium (ATCC 13311), Salmonella enterica subsp. enterica sorovar. Typhi (ATCC 19214) e Shigella flexneri (ATCC 12022). The ethanolic solution at 30% of coumarin has significant inhibitory effect on Gram positive bacteria, showing MIC <2µg/mL for S. epidermidis, B. cereus and L. monocytogenes.

Analysis of the Essential Oil of Artemisia Absinthium in Two Harvest Times

“…Coumarins are a class of chemicals produced by plants that have biological activities traditionally known for the treatment of respiratory diseases, due to bronchodilator, expectorant, anti-inflammatory and antiallergic actions (Silva et al 2016, Czelusniak et al 2012. In plants these substances can have ecological function in response to physical, chemical or biological stress, being part of the chemical defense of plants for resistance to different pathogens (Mazaro et al 2008, Mazaro et al 2013, Stangarlin et al 2010, Stangarlin et al 2011).…”