Solubility prediction in octanol: A technical note

Sepassi, Kia; Yalkowsky, Samuel H.

doi:10.1208/pt070126

Cited by 30 publications

(21 citation statements)

References 15 publications

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“…Octanol was chosen as primary oil phase composition due to its low Hildebrand solubility parameter γ of 21.1 (J/cm 3 ) 0.5 (Sepassi and Yalkowsky, 2006). Ethyl acetate was used to modify the interfacial tension at the oil-water interface during amelogenin self-assembly (vanBuuren et al, 1996).…”

Section: Methodsmentioning

confidence: 99%

Self-aligning amelogenin nanoribbons in oil–water system

Martinez-Avila

et al. 2011

Journal of Structural Biology

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The highly organized microstructure of dental enamel is a result of protein-guided anisotropic growth of apatite nanofibers. It is established that amelogenin proteins, the main constituent of the developing enamel matrix, form nanospheres in vitro, but the amphiphilic nature of the full-length protein conveys the possibility of generating more complex structures as observed with other surfactant-like molecules. This study tested if the use of metastable oil-water emulsions can induce supramolecular assemblies of amelogenin. Recombinant full-length amelogenin, rH174, was mixed into octanol/ethyl acetate preparations of different ratios to form emulsions at pH 4.5 and 7.4. Atomic force and electron microscopy showed the formation of 16.7±1.0 nm wide nanoribbons which grew to several micrometer length over a period of days. Nanoribbons formed from reverse micelles by enabling hydrophobic tails of the molecules to interact while preventing the formation of amelogenin nanospheres. Ribbon formation required the presence of calcium and phosphate ions and may be localized at a dark central line along the amelogenin ribbons. The ribbons have a strong tendency to align in parallel maintaining 5 to 20nm space between each other. The growth rates and number of ribbons were significantly higher at pH 4.5 and related to the metastability of the emulsion. A model for ribbon extension proposes the addition of short segments or amelogenin dimers to the ends of the ribbon. The formation of self-aligning and uniaxially elongating amelogenin structures triggered by the presence of calcium and phosphate may represent a suitable new model for protein controlled mineralization in enamel.

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Section: Methodsmentioning

confidence: 99%

Self-aligning amelogenin nanoribbons in oil–water system

Martinez-Avila

et al. 2011

Journal of Structural Biology

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“…Yalkowsky et al (1983) (10) which was tested on the solubility of 12 pharmaceuticals and the mean percentage deviation was 85.1 (± 21.6) % (Jouyban, 2009). Sepassi and Yalkowsky (2006) (Stovall et al, 2005b). Figure 4 where the solubility of gases decreases with increased temperature.…”

Section: Solubility In Organic Solventsmentioning

confidence: 99%