Solubility of plasma proteins in the presence of polyethylene glycol

Haskó, F.; Vaszileva, Roza; Halász, László

doi:10.1002/bit.260240902

Cited by 36 publications

(12 citation statements)

References 13 publications

(2 reference statements)

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“…For characterization of the magnetite particles, the size distribution and zeta‐potentials of magnetite particles were determined using a dynamic light scattering spectrophotometer (FRAR 1000; Otsuka Electronics, Osaka, Japan)18 and a zeta‐potential analyzer (ELSZ‐2; Otsuka Electronics), respectively. The PEG concentration was measured according to a method described by Vaszileva and Haskó 19. Briefly, 1.0 mL of 0.5 M perchloric acid was added to 0.2 mL of sample solution, and the mixture was allowed to stand for 15 min, followed by centrifugation at 10,000 × g for 4 min.…”

Section: Methodsmentioning

confidence: 99%

Cell‐patterning using poly (ethylene glycol)‐modified magnetite nanoparticles

Akiyama

Ito

Kawabe

et al. 2009

J Biomedical Materials Res

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Development of cell-patterning techniques is a major challenge for the construction of functional tissues and organs in tissue engineering. Recent progress in surface chemistry has enabled spatial control of cell adhesion onto cultural substrates by varying hydrophilicity, for example, by using poly (ethylene glycol) (PEG). In the present study, we developed a novel cell-patterning procedure using PEG-modified magnetite particles (PEG-Mags) and magnetic force. Using an array-patterned magnet, PEG-Mags were magnetically patterned on the surface of a tissue culture dish. The resultant substrate surface consisted of two regions: the PEG-Mag surface that acts as a cell-resistant region and the native substrate surface that promotes cell adhesion. When human keratinocyte HaCaT cells were seeded onto the PEG-Mag-patterned surface, cells adhered only to the native substrate surface, resulting in cell-patterning on the tissue culture dish. The patterned PEG-Mags were then washed away to expose the native substrate surface, and thereafter, when mouse myoblast C2C12 cells were seeded to the dish, cells adhered to the exposed substrate surface, resulting in a patterned coculture of heterotypic cells. Moreover, it is worth noting that the magnetic force-based cell-patterning procedure is not limited by the property of cultural substrate surfaces, and that cell-patterning of mouse fibroblast NIH3T3 cells on a monolayer of HaCaT cells was successfully achieved using PEG-Mags and magnetic force. These results indicate that this procedure provides a novel concept for cell-patterning and may be useful for tissue engineering and cell biology.

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Section: Methodsmentioning

confidence: 99%

Cell‐patterning using poly (ethylene glycol)‐modified magnetite nanoparticles

Akiyama

Ito

Kawabe

et al. 2009

J Biomedical Materials Res

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“…Precipitations were performed at different pH values (4.5, 5.0, and 5.5), with different salts (sodium acetate and sodium citrate), at different salt concentrations (5,10,20,40,50,100,125, and 200 mM), and different caprylic acid concentrations (0.5, 1.0, 2.0, 4.0, 6.0, and 10.0%). The solution mixing parameters were also varied, including the mixing time (15,30,45, 60 min) and rotation speed (10, 20, 30, and 40 rpm).…”

Section: Parameter Optimizations Of Caprylic Acid/peg Precipitationsmentioning

confidence: 99%

“…The precipitation of proteins with PEG occurs in a volume exclusion reaction [16,17]. In recent years, further research was performed to determine the solubility curve of common proteins during the precipitation process [18][19][20][21][22][23]. Precipitation by PEG does not allow removal of high molecular weight impurities (HMWIs), like DNA [24] or aggregates, because the precipitation of larger proteins tends to require lower PEG concentrations and shorter http://dx.doi.org/10.1016/j.bej.2014.…”

Section: Introductionmentioning

confidence: 99%

Capture and intermediate purification of recombinant antibodies with combined precipitation methods

Sommer

Tscheliessnig

Satzer

et al. 2015

Biochemical Engineering Journal

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“…The fundamentals of protein precipitation by PEG were established in the early 1960s (Polson et al, ) and since then has been extensively studied to understand the protein behavior (Atha and Ingham, ; Hasko et al, ). PEG is a polar uncharged polymer and can be considered as a viable option for precipitation of antibodies.…”

Section: Precipitation Technologiesmentioning

confidence: 99%

Clarification technologies for monoclonal antibody manufacturing processes: Current state and future perspectives

Singh

Arunkumar

Chollangi

et al. 2015

Biotech & Bioengineering

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Considerable progress has been made increasing productivity of cell cultures to meet the rapidly growing demand for antibody biopharmaceuticals through increased cell densities and longer culture times. This in turn has dramatically increased the burden of process and product related impurities on the purification processes. In addition, current trends in the biopharmaceutical industry point toward both increased productivity and targeting smaller patient populations for new indications. Taken together, these developments are driving the industry to explore alternative separation technologies as a future manufacturing strategy. Clarification technologies well established in other industries, such as flocculation and precipitation are increasingly considered as a viable solution to address this bottleneck in antibody processes. However, several technical issues need to be fully addressed including suitability as a platform application, robustness, process cost, toxicity, and clearance. This review will focus on recent efforts to incorporate new generation clarification technologies for mammalian cell cultures producing monoclonal antibodies as well as challenges to their implementation supported by a case study.

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Solubility of plasma proteins in the presence of polyethylene glycol

Cited by 36 publications

References 13 publications

Cell‐patterning using poly (ethylene glycol)‐modified magnetite nanoparticles

Cell‐patterning using poly (ethylene glycol)‐modified magnetite nanoparticles

Capture and intermediate purification of recombinant antibodies with combined precipitation methods

Clarification technologies for monoclonal antibody manufacturing processes: Current state and future perspectives

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