Cell‐patterning using poly (ethylene glycol)‐modified magnetite nanoparticles

Abstract

Development of cell-patterning techniques is a major challenge for the construction of functional tissues and organs in tissue engineering. Recent progress in surface chemistry has enabled spatial control of cell adhesion onto cultural substrates by varying hydrophilicity, for example, by using poly (ethylene glycol) (PEG). In the present study, we developed a novel cell-patterning procedure using PEG-modified magnetite particles (PEG-Mags) and magnetic force. Using an array-patterned magnet, PEG-Mags were magnetically patterned on the surface of a tissue culture dish. The resultant substrate surface consisted of two regions: the PEG-Mag surface that acts as a cell-resistant region and the native substrate surface that promotes cell adhesion. When human keratinocyte HaCaT cells were seeded onto the PEG-Mag-patterned surface, cells adhered only to the native substrate surface, resulting in cell-patterning on the tissue culture dish. The patterned PEG-Mags were then washed away to expose the native substrate surface, and thereafter, when mouse myoblast C2C12 cells were seeded to the dish, cells adhered to the exposed substrate surface, resulting in a patterned coculture of heterotypic cells. Moreover, it is worth noting that the magnetic force-based cell-patterning procedure is not limited by the property of cultural substrate surfaces, and that cell-patterning of mouse fibroblast NIH3T3 cells on a monolayer of HaCaT cells was successfully achieved using PEG-Mags and magnetic force. These results indicate that this procedure provides a novel concept for cell-patterning and may be useful for tissue engineering and cell biology.

Neurons sense nanoscale roughness with nanometer sensitivity

“…In fact, because the adhesion sites of the cell (focal adhesions) are in the range of 5-200 nm, it is clear that these very small cellular components may be strongly influenced by nanoscale features rather than microscale structures (18). In this frame, nanofabrication techniques may offer interesting tools to achieve a precise control of the surface properties (e.g., controlled nanotexture) and, thus, to evaluate the mechanisms and spatiotemporal aspects of nanomaterial interactions with living systems (19)(20)(21)(22)(23). In this work, we investigated the biological response of human neuroblastoma cells (SH-SY5Y) upon interaction with highly controlled nanostructured metal substrates.…”

Magnetic Nanoparticles in Tissue Regeneration

“…In this approach, cells bind to cell-attractive substrate developed in the form of vessel or circular grooves using array-patterned magnet source. Akiyama et al [153] have used PEG-MNPs for such type of patterning. The average size of PEG-MNPs was 220 nm.…”

“…The average size of PEG-MNPs was 220 nm. Surface of MNPs was first modified by aminosilane, further which was used to couple the polyethylene glycol (PEG) and successfully used to develop a layered co-culture technique using HaCaT cells and mouse myoblast C2C12 cells [153]. These experiments are an insight idea about the use of such techniques with possible improvement that could lead to successful tissue regeneration.…”

Magnetic Assembly of Tissue Surrogates

“…Edited by Jamie Davies. 44 field required for cell manipulation is normally in the sub-Tesla range, which is relatively low and does not result in detectable adverse effects on cells 8 . Most magnetic materials for cell labelling have been widely used for many years in numerous biomedical applications including cell separation 9 , drug delivery 10 , or contrast enhancement in magnetic resonance imaging 11 , and are generally considered safe to human cells.…”