Solid support resins and affinity purification mass spectrometry

Havis, Spencer; Moree, Wilna J.; Mali, Sujina; Bark, Steven J.

doi:10.1039/c6mb00735j

Cited by 7 publications

(2 citation statements)

References 50 publications

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“…Further procedures can then be performed (such as MS) to identify the interacting protein. AP is similar to co-immunoprecipitation; instead of immobilized antibodies, the bait protein is immobilized on the bead ( Havis et al, 2017 ). Tandem AP has a few additional steps compared to AP.…”

Section: Recommendation Of Approach For the Identification And Elucid...mentioning

confidence: 99%

Uncovering Novel Viral Innate Immune Evasion Strategies: What Has SARS-CoV-2 Taught Us?

et al. 2022

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The ongoing SARS-CoV-2 pandemic has tested the capabilities of public health and scientific community. Since the dawn of the twenty-first century, viruses have caused several outbreaks, with coronaviruses being responsible for 2: SARS-CoV in 2007 and MERS-CoV in 2013. As the border between wildlife and the urban population continue to shrink, it is highly likely that zoonotic viruses may emerge more frequently. Furthermore, it has been shown repeatedly that these viruses are able to efficiently evade the innate immune system through various strategies. The strong and abundant antiviral innate immunity evasion strategies shown by SARS-CoV-2 has laid out shortcomings in our approach to quickly identify and modulate these mechanisms. It is thus imperative that there be a systematic framework for the study of the immune evasion strategies of these viruses, to guide development of therapeutics and curtail transmission. In this review, we first provide a brief overview of general viral evasion strategies against the innate immune system. Then, we utilize SARS-CoV-2 as a case study to highlight the methods used to identify the mechanisms of innate immune evasion, and pinpoint the shortcomings in the current paradigm with its focus on overexpression and protein-protein interactions. Finally, we provide a recommendation for future work to unravel viral innate immune evasion strategies and suitable methods to aid in the study of virus-host interactions. The insights provided from this review may then be applied to other viruses with outbreak potential to remain ahead in the arms race against viral diseases.

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Section: Recommendation Of Approach For the Identification And Elucid...mentioning

confidence: 99%

Uncovering Novel Viral Innate Immune Evasion Strategies: What Has SARS-CoV-2 Taught Us?

et al. 2022

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“…These methods are often complex, expensive, time consuming and require a significant level of expertise for performing them in a reliable manner. AP-MS and CoAP-MS (co-affinity purification mass spectroscopy) are actively studied today from separations point of view as well [39][40][41], while MS-based mapping of protein-protein interactions is progressing more slowly [42,43].…”

Section: Challenges In Separation/purification Of Human Proteins In Tmentioning

confidence: 99%

Challenges in Separations of Proteins and Small Biomolecules and the Role of Modern Mass Spectroscopy Tools for Solving Them, as Well as Bypassing Them, in Structural Analytical Studies of Complex Biomolecular Mixtures

Haramija

2018

Separations

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State-of-the-art purification of biomolecules, as well as separation of complex omic mixtures, is crucial for modern biomedical research. Mass spectroscopy (MS) represents a technique that both requires very clean biomedical samples and can substantially assist liquid chromatography (LC) separations, using either LC-MS or LC-MS/MS methods available. Here, a brief overview of the applicability of LC-MS/MS methodology for structural analyses of complex omic mixtures without prior purification of each sample component will be given. When necessary bioinformatic tools are available, these can be carried out quite quickly. However, manual data analysis of such complex mixtures is typically very slow. On the other hand, the need for high-level purity of protein samples for modern biomedical research will be discussed. Often, modification of protein purification protocols is needed, or additional purification steps may be either required or preferred. In the context of mass spectroscopy-related biomedical research, purification of pmol and subpmol amounts of biomedical samples, as well as commercial availability of pmol amounts of purified standards will be discussed.

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Combination of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Substrate Trapping for the Detection of Transient Protein Interactions

Duda¹,

Thomas²

2022

Methods in Molecular Biology

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Solid support resins and affinity purification mass spectrometry

Cited by 7 publications

References 50 publications

Uncovering Novel Viral Innate Immune Evasion Strategies: What Has SARS-CoV-2 Taught Us?

Uncovering Novel Viral Innate Immune Evasion Strategies: What Has SARS-CoV-2 Taught Us?

Challenges in Separations of Proteins and Small Biomolecules and the Role of Modern Mass Spectroscopy Tools for Solving Them, as Well as Bypassing Them, in Structural Analytical Studies of Complex Biomolecular Mixtures

Combination of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Substrate Trapping for the Detection of Transient Protein Interactions

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