Combination of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Substrate Trapping for the Detection of Transient Protein Interactions

Interactions of Histone Deacetylase 6 with DNA Damage Repair Factors Strengthen its Utility as a Combination Drug Target in High-Grade Serous Ovarian Cancer

“…In order to consider HDACs as a therapeutic target in HGSOC we need to better understand their function in HGSOC, including their protein–protein interactions. Applying a catalytically inactive mutant allows substrates to bind to enzymes for a longer period of time and enables the detection of interacting proteins, including transient interactors, using immunoprecipitation coupled with mass spectrometry analysis, as described previously. − Mass spectrometry-basedanalysis can facilitate a comprehensive understanding of the HGSOC proteome, thereby enabling the elucidation of functional consequences of genomic changes . This analytical approach can also enable the identification of new therapeutic vulnerabilities to decrease drug resistance.…”