Solid-state NMR studies for the development of non-woven biomaterials based on silk fibroin and polyurethane

Nakazawa, Chikako T.; Higuchi, Akira; Asano, Atsushi; Kameda, Tsunenori; Aytemiz, Derya; Nakazawa, Yasumoto

doi:10.1038/pj.2017.16

Cited by 13 publications

(9 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A porous structure is desirable for cell and tissue immigration and the growth of tissue engineering materials because the extracellular matrix (ECM) of native tissue has a similar structure [19]. The electrospun SF/PU composite patches were evaluated by gross observation and their morphology was characterized using a SEM operated at 10 kV with a 1000× magnification (VE-7800 Keyence Co., Osaka, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…The 13 C spectra were obtained by the combined use of cross polarization (CP) and MAS. The contact time of CP was 1 ms. Proton spin-lattice relaxation times in the laboratory frame ( T 1 H ) were indirectly measured from the well-resolved 13 C signals enhanced by CP of 1 ms applied after the inversion-recovery pulse sequence for 1 H [19].…”

Section: Methodsmentioning

confidence: 99%

“…In our previous report, we presented that the ordered structures of SF and PU are preserved in the blend sample after electrospining. For SF, the inter- and intra-molecular hydrogen bonds maintained the β-sheet structure, and the interaction was not severely affected by the presence of PU [19]. Further, samples were evaluated by in vivo by implantation into the abdominal aortas of rats, using the ePTFE patch in the control group.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Compatibility Evaluation of Non-Woven Sheet Composite of Silk Fibroin and Polyurethane in the Wet State

et al. 2018

Self Cite

View full text Add to dashboard Cite

SF/polyurethane composite non-woven sheet was fabricated to evaluate the cardiovascular tissue engineering materials in the wet state. The compatibility and microstructure analyses were carried out on the fabricated SF/polyurethane composite non-woven sheet by thermal analysis and solid-state NMR analysis in the wet state. To evaluate the modulus of elasticity, a tensile test was performed and supported with dynamic viscoelasticity and mechanical analysis. Results showed that SF/polyurethane composites form domains within the non-woven sheet and are in a finely dispersed state while maintaining their structures at a scale of several tens of nm. Moreover, an increase of the loss tangent with low elastic modulus proved that a micromolecular interaction occurs between silk fibroin (SF) and polyurethane molecules.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Compatibility Evaluation of Non-Woven Sheet Composite of Silk Fibroin and Polyurethane in the Wet State

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…The majority of these works use solvents unaddressed or belonging to Class 1 (Solvents to be avoided) in the European Pharmacopoeia (14)- (20), (35) (e.g. hexa-fluoro-isopropanol, also characterized by exorbitant costs, extreme volatility and difficult manageability), or polyurethanes that are degradable and/or not suitable for long-term medical application (18)- (28) (36) , which would unlikely pass the exam of regulatory approval necessary for product certification and commercialization.…”

Section: Introductionmentioning

confidence: 99%

Electrospun fibroin/polyurethane hybrid meshes: Manufacturing, characterization, and potentialities as substrates for haemodialysis arteriovenous grafts

et al. 2018

View full text Add to dashboard Cite

Several attempts made so far to combine silk fibroin and polyurethane, in order to prepare scaffolds encompassing the bioactivity of the former with the elasticity of the latter, suffer from critical drawbacks concerning industrial and clinical applicability (e.g., separation of phases upon processing, use of solvents unaddressed by the European Pharmacopoeia, and use of degradable polyurethanes). Overcoming these limitations, in this study, we report the successful blending of regenerated silk fibroin with a medical-grade, non-degradable polyurethane using formic acid and dichloromethane, and the manufacturing of hybrid, semi-degradable electrospun tubular meshes with different ratios of the two materials. Physicochemical analyses demonstrated the maintenance of the characteristic features of fibroin and polyurethane upon solubilization, blending, electrospinning, and postprocessing with ethanol or methanol. Envisioning their possible application as semidegradable substrates for haemodialysis arteriovenous grafts, tubular meshes were further characterized, showing submicrometric fibrous morphologies, tunable mechanical properties, permeability before and after puncture in the same order of magnitude as commercial grafts currently used in the clinics. Results demonstrate the potential of this material for the development of hybrid, new-generation vascular grafts with disruptive potential in the field of in situ tissue engineering. © 2018 Wiley Periodicals, Inc. J. Biomed. Mater. Res. Part B, 2018.

show abstract

“…We believe that the high compatibility between SPU and SF helps to create a uniform and organized structure with low deviation between different samples. However, decreasing the SF concentration resulted in a more disorganized structure; this is attributed to high stress–strain deviation within these samples …”

Section: Resultsmentioning

confidence: 93%

Relationship between structure and physical strength of silk fibroin nanofiber sheet depending on insolubilization treatment

Fukuda

Aytemiz

Higuchi

et al. 2017

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

Materials such as expanded polytetrafluoroethylene and glutaraldehyde-fixed bovine pericardium are currently used for cardiac tissue regeneration. However, patches made from these materials remain permanently without being absorbed by the body and must be replaced overtime because of degeneration or lack of growth. To improve the long-term outcomes for cardiac tissue regeneration, biocompatible and biodegradable materials must be used. In this study, we used two biocompatible polymers, silk fibroin (SF), which is biodegradable and segmented polyurethane, to prepare nonwoven sheets that were then insolubilized by water vapor or methanol treatment. The tensile stress increased significantly on adding segmented polyurethane to pure SF and the water vapor processed sheets showed higher extension on strain than the methanol-processed sheets. The different insolubilization treatments also resulted in different SF structures. Our results show that it is possible to obtain the physical properties required for cardiac tissue repair patch by varying the insolubilization treatment.

show abstract

Solid-state NMR studies for the development of non-woven biomaterials based on silk fibroin and polyurethane

Cited by 13 publications

References 14 publications

Compatibility Evaluation of Non-Woven Sheet Composite of Silk Fibroin and Polyurethane in the Wet State

Compatibility Evaluation of Non-Woven Sheet Composite of Silk Fibroin and Polyurethane in the Wet State

Electrospun fibroin/polyurethane hybrid meshes: Manufacturing, characterization, and potentialities as substrates for haemodialysis arteriovenous grafts

Relationship between structure and physical strength of silk fibroin nanofiber sheet depending on insolubilization treatment

Contact Info

Product

Resources

About