Solid-state fermentation of brewer’s spent grain for xylanolytic enzymes production by Penicillium janczewskii and analyses of the fermented substrate

Terrasan, César Rafael Fanchini; Carmona, Eleonora Cano

doi:10.14393/bj-v31n6a2015-30044

Cited by 26 publications

(13 citation statements)

References 22 publications

(26 reference statements)

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“…The average value of total organic carbon measured in BSG used in this research was 55.5% dry matter or DM and it is in line with literature data (52.3% DM ) (Mathias 2015). Literature data on lignin content in BSG vary from 5.14 to 27.8% DM , while the average value gained in this study was 8.53% DM . Literature data on the cellulose content vary from 14.7 to 26.8% DM while the measured content in this study was 16.1% DM .…”

Section: Resultsmentioning

confidence: 60%

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Biovalorization of brewers’ spent grain for the production of laccase and polyphenols

et al. 2018

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Brewers’ spent grain (BSG) is the main solid by‐product of the brewing process and is typically disposed of as cattle feed. In this study, BSG was evaluated as a substrate for the production of polyphenols and the lignin‐degrading enzyme laccase using fungal solid‐state fermentation by Trametes versicolor. Laccases are finding increasing applications in the food industry and polyphenols have benefits for human health. After 14 days of fermentation with T. versicolor, there was a 3.4‐fold increase in the extraction of total polyphenols compared with untreated BSG. Using BSG as the sole source of carbon and nitrogen, maximum laccase activity was achieved after seven days of treatment with an activity of 560 U/L. Based on these results, BSG is suggested to be a good lignocellulose waste material to produce value‐added products such as the enzyme laccase and polyphenols. Copyright © 2018 The Institute of Brewing & Distilling

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Section: Resultsmentioning

confidence: 60%

“…. Recent publications suggest diverse applications of BSG as a substrate for the cultivation of microorganisms , the production of protein concentrates , enzymes , citric acid, lactic acid and xylitol or as an adjunct for human food products .…”

Section: Introductionmentioning

confidence: 99%

Biovalorization of brewers’ spent grain for the production of laccase and polyphenols

et al. 2018

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“…Filamentous fungi are known to be good producers of extracellular xylanases by solid-state fermentation (SSF) using lignocellulosic agricultural by-products as components of microbial growth media, with several economic advantages: low water output, cheap substrates, low risk of contamination and low operating costs (MORETTI et al, 2012;TERRASAN;CARMONA, 2015). Among these residues, sugarcane bagasse is one of the most important in Brazil and is composed by approximately 50% cellulose, 25% hemicellulose and 20% lignin (PANDEY et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Gomes¹,

Santos²,

Franciscon³

et al. 2016

Biosci. J.

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This study reports the optimization of xylanase production under solid state fermentation (SSF) by a thermotolerant Aspergillus fumigatus strain (SCB4) isolated from sugarcane bagasse piles of Brazilian Cerrado. Different combinations of low-cost agricultural byproducts in SSF were evaluated: sugarcane bagasse and wheat bran (1:1), sugarcane bagasse and corn straw (1:1) and only sugarcane bagasse. The enzyme biosynthesis by SSF was carried out at different temperatures (40, 45, 50 and 55 o C). The maximum levels of xylanase activity were obtained after 24 h at 45 °C using a culture medium containing sugarcane bagasse and wheat bran (1:1). Under optimal conditions, the fungal culture produced 574 U g -1 of xylanase (units/g of dry substrate). The crude enzyme showed optimal activity at 60 °C and pH 4.5. It exhibited thermostability up to 55 °C, wide range of pH stability and tolerance to ethanol, xylose and glucose. The physicochemical properties shown by this enzyme are appropriate for its application in hydrolysis of lignocellulosic residues for ethanol production and other bioproducts.

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“…This catalytic property enables these enzymes to be used in the hydrolysis of vegetal biomass for the production of biofuels, the formulation of animal feed, or even in bread-making processes to improve the quality of dough, hydrolyzing xylooligosaccharides that are disadvantageous to the formation of gluten and impairs the crumb structure (BECKER et al, 2009;BRIENZO et al, 2012;OLIVEIRA et al, 2014;PIROTA et al, 2015;TERRASAN and CARMONA, 2015). However, the biobleaching of kraft pulp during the processing of cellulose for paper production is the more widespread and desired use of xylanolytic enzymes, due of the economic importance of this industrial segment (MASUI et al, 2012;MITTAL et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…An alternative for reducing the final cost of these biomolecules involves the use of agro-industrial residues in solidstate fermentation to produce microbial enzymes (BRIENZO et al, 2012;MASUI et al, 2012;SILVA et al, 2013;TERRASAN and CARMONA, 2015). Approximately 40% of the final cost of an enzyme is derived from the medium used for microorganism cultivation (ROMERO et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Production of xylanase by a new strain of Thermoascus aurantiacus: obtainment of enzymatic extract with reduced cellulolytic activity for application in pulp and paper industries

Costa¹,

Scalabrini²,

Silvestre³

et al. 2016

Biosci. J.

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ABSTRACT:Xylanases are useful in several industrial segments, including pulp and paper bleaching, animal feed, and bread-making processes. However, the industrial use of these enzymes is closely related to its production cost and its catalytic properties. The process of solid state fermentation enables the use of agro-industrial residues as substrates for microbial cultivation and enzymes production, reducing costs. In the present study, different cultivation parameters were evaluated for the xylanase production by the thermophilic fungus Thermoascus aurantiacus, by solid state fermentation, using agro-industrial residues as substrates. High production of xylanase (1701.9 U g -1 of dry substrate) was obtained using wheat bran containing 65% of initial moisture, at 120 h of cultivation, and 45°C. The xylanase showed optimal activity at pH 5.0 and 75°C; its stability was maintained at pH 3.0-11.0. The enzyme retained its catalytic potential after 1 h, at 75°C. The enzymatic extract produced under optimized conditions showed reduced activities of endoglucanase and FPase. Our results, including the xylanase production by T. aurantiacus in low-cost cultivation medium, high structural stability of the enzyme, and reduced cellulolytic activity, encourage the application of this enzymatic extract in pulp and paper bleaching processes.

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Solid-state fermentation of brewer’s spent grain for xylanolytic enzymes production by Penicillium janczewskii and analyses of the fermented substrate

Cited by 26 publications

References 22 publications

Biovalorization of brewers’ spent grain for the production of laccase and polyphenols

Biovalorization of brewers’ spent grain for the production of laccase and polyphenols

Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Production of xylanase by a new strain of Thermoascus aurantiacus: obtainment of enzymatic extract with reduced cellulolytic activity for application in pulp and paper industries

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