Abstract:A laboratory-scale study of bioconversion of local lignocellulosic material, oil palm biomass (OPB) was conducted by evaluating the enzyme production through microbial treatment in solid state bioconversion (SSB). OPB in the form of empty fruit bunches (EFB) was used as a solid substrate and treated with the white-rot fungus, Phanerochaete chrysosporium, to produce ligninase. The results showed that the highest ligninase activity of 400.27 U/liter was obtained at day 12 of fermentation. While the optimum study… Show more
In the present study, various white-rot fungi were used for the pretreatment of oil palm empty fruit bunch (EFB) using solid-state cultivation. The results showed that Trametes versicolor TISTR 3224 gave the highest selectivity value (the ratio of lignin degradation to cellulose degradation) of 1.57. In comparison, Trametes sp. BCC 8729, Phanerochaete chrysosporium ATCC 24725, Marasmius sp. BCC 9542 and Xylaria sp. BCC 7749 gave selectivity of 0.60, 0.59, 0.30 and 0.06, respectively. Screening parameters for the fungal pretreatment of EFB using T. versicolor TISTR 3224 was studied by Plackett-Burman design (PBD). It indicated that the moisture content and co-substrate gave a positive effect on the lignin degradation, while EFB concentration had a negative effect on cellulose degradation. The optimum conditions for lignin degradation obtained from Box-Behnken statistical experimental design (BBD) were 80 % moisture content, 2.29 % wheat flour and 23.3 % EFB. Under this condition, 15.6 % of delignification was obtained. After an enzymatic hydrolysis, the digestibility of fungal treated EFB under the optimum condition achieved 1.34-fold compared with untreated EFB. Keywords Box-Behnken design Á Plackett-Burman design Á Fungal pretreatment Á Oil palm empty fruit bunch (EFB) Á Deliginification
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