Solid-Phase Radioimmunoassay of Estradiol-17β

Abraham, Guy E.

doi:10.1210/jcem-29-6-866

Cited by 849 publications

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“…The specificity of the two antisera was tested against several steroids; the crossreaction is expressed as the proportional ratio of the mass of steroid and interfering compound, at 50% displacement of the tracer (Abraham, 1969). Table 1 shows the results for the progesterone antiserum, Table 2 for the estrone antiserum.…”

Section: Results Radioimmunoassaysmentioning

confidence: 99%

Radioimmunoassays to determine the presence of progesterone and estrone in the starfish Asterias rubens

Dieleman

Schoenmakers

1979

General and Comparative Endocrinology

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RIA's have been made for progesterone and estrone with the antisera S74B7 and 7604-7 40, respectively, which will be described. The characteristics of the RIA for progesterone resemble those of other reported RIA's. The antiserum for the RIA of estrone is highly specific, with main cross-reactions of 0.32%. 0.27% and 0.13% for estradiol-17P, estradioLl7a and oestriol, respectively. The RIA for estrone is sensitive (affinity constant: 28.2 x 10" l/mol), with a standard curve in the range from 1 to 10 pg. Accuracy and precision were reliable, as proved by the significant correlation between steroid added and steroid estimated.With the use of these RIA's. progesterone and estrone were demonstrated in the ovaries and pyloric ceca of single specimens of Asterias rubens. Progesterone was also present in the perivisceral fluid, but estrone appeared to be absent. The progesterone level was lower and the estrone level higher in the ovaries than in the pyloric ceca.The significance of the presence of these steroids in the ovaries and pyloric ceca of Asrerias rubens,

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Section: Results Radioimmunoassaysmentioning

confidence: 99%

Radioimmunoassays to determine the presence of progesterone and estrone in the starfish Asterias rubens

Dieleman

Schoenmakers

1979

General and Comparative Endocrinology

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“…At the same time, varying amounts of unlabelled potentially crossreacting steroids from 25 pg to 100 ng were reacted as for the standard curve procedure and compared with results for the measurements with the homologous hormone. The percentage cross-reactivity was calculated as described (Abraham, 1969). The cross-reaction of the anti-oestradiol serum was below 1% with oestrone or oestriol, and below 0.10% with testosterone, androstenedione, progesterone, 1 7a-hydroxy progesterone and cortisol.…”

Section: Methodsmentioning

confidence: 99%

Immunofluorescent detection of hormone receptors in cutaneous melanocytic tumours

Thompson¹,

Cook²,

Gill³

1981

Br J Cancer

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Summary.-Immunofluorescent assessment of hormone receptors in melanocytic tumours is quite feasible without loss of diagnostic material, in contrast to the impracticability of quantitative biochemical assays. Using this method, oestrogen receptors were demonstrated in 4/6, and progesterone receptors in 3/5 patients with metastatic melanoma. Receptors were not found in 3 patients with primary cutaneous melanomas of the superficial spreading type. Progesterone receptors were present in the junctional component of a naevus from one healthy person.

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“…Plasma 17ft-oestradiol was determined by the solid-phase radioimmunoassay reported by Abraham (1969). Determinations were performed in triplicate on O· 5-ml aliquots of plasma and the values obtained were corrected for extraction losses.…”

Section: Methodsmentioning

confidence: 99%

Effect of Progesterone and Oestrogen on the Survival and Development of Fertilized Ova in the Ovariectomized Ewe

Trounson¹,

Moore²

1974

Aust. Jnl. Of Bio. Sci.

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Two experiments were carried out to investigate the importance of progesterone and oestrogen for the survival and development of fertilized ova in ovariectomized ewes. In experiment 1 ewes were treated with progesterone (P) alone or progesterone and 17 p-oestradiol (P + E2) following ovariectomy on the second (day 2) or sixth day (day 6) after mating to vasectomized rams. Fertilized ova were transferred to the ewes either immediately after ovariectomy or on day 6 after ovariectomy on day 2. In experiment 2 ewes that had been given an equine anterior pituitary extract to induce multiple ovulation were ovariectomized on day 2 after mating to fertile rams, and then treated with P or P+E2. On day 18 the uteri of all ewes were flushed to recover embryos. Treatment with P commenced immediately after ovariectomy and E2 was given from day 21-to day 5, to ewes ovariectomized on day 2. Ovarian vein blood was collected just prior to ovariectomy and was assayed for its progesterone and oestrogen contents.In both experiments oestradiol had no effect upon the proportion of ewes with normal embryos. In experiment 1 delay of transfer until day 6 after ovariectomy on day 2 increased the proportion of ewes with normal embryos [28 of 43 (65 %) v. 16 of 46 (35 %)]. Transfers on day 6 after ovariectomy on the same day tended to be less successful than transfers on day 6 after ovariectomy on day 2 [11 of 23 (48 %) v. 28 of 43 (65 %)]. In all ewes in experiment 2, and in experiment 1 where ovariectomies and transfers were carried out on the same day, survival and normal development of ova occurred only in ewes in which the ovarian vein progesterone levels at ovariectomy, expressed as progesterone per corpus luteum, fell within precise limits. There was no such relationship in ewes ovariectomized on day 2 and given progesterone prior to transfer on day 6.It would appear that the secretion of oestradiol during the luteal phase of the oestrous cycle in the ewe has little effect on the survival and development of ova. However, progesterone appears to have an important role. Further, it would appear that as early as the second day after oestrus the activity of the corpus luteum, as assessed by its progesterone secretion, may well influence the success or failure of pregnancy.

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Solid-Phase Radioimmunoassay of Estradiol-17β

Cited by 849 publications

References 0 publications

Radioimmunoassays to determine the presence of progesterone and estrone in the starfish Asterias rubens

Radioimmunoassays to determine the presence of progesterone and estrone in the starfish Asterias rubens

Immunofluorescent detection of hormone receptors in cutaneous melanocytic tumours

Effect of Progesterone and Oestrogen on the Survival and Development of Fertilized Ova in the Ovariectomized Ewe

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