Radioimmunoassays to determine the presence of progesterone and estrone in the starfish Asterias rubens

Dieleman, S.J.; Schoenmakers, H.J.N.

doi:10.1016/0016-6480(79)90242-9

Cited by 89 publications

(33 citation statements)

References 23 publications

(25 reference statements)

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“…Dieleman and Schoenmakers (1979) demonstrated by radioimmunoassay (RIA) the presence of progesterone and oestrone in these organs in agreement with earlier reports on the presence of progesterone (Botticelli et al, 1960(Botticelli et al, , 1961Ikegami et al, 1971) and of substances with oestrogen-like activity based on bioassays (Steidle, 1930;Donahue, 1940;Hagerman et al, 1957;Botticelli et al, 1960Botticelli et al, , 1961 in the ovaries of other echinoderms.…”

Section: Introductionsupporting

confidence: 91%

Progesterone and oestrone levels in the gonads and pyloric caeca of the male sea star Asterias rubens: A comparison with the corresponding levels in the female sea star

Voogt

Dieleman

1984

Comparative Biochemistry and Physiology Part A: Physiology

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Abstract-l.Levels of progesterone and oestrone were estimated by means of RIA in the gonads and pyloric caeca of male specimens of Asterius rubens throughout the reproductive cycle. 2. The patterns obtained appeared to be sex-specific on comparison with those of female specimens of A. rubens and it was concluded that progesterone and oestrone in the testes are involved in the regulation of gametogenesis.3. The levels of progesterone in the pyloric caeca were about ten times higher than those in the testes; in the same sequence levels of oestrone were only slightly higher.4. The onset of a new reproductive cycle, taking the abrupt decrease of the pyloric caeca-index as a marker, coincides with a strong decrease of the progesterone level (P~O.001) and an increase of the oestrone level (P < 0.01) in the pyloric caeca.5. It is supposed that progesterone and oestrone in the pyloric caeca are concerned with making available the materials required for gametogenesis INTRODUCTION

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Section: Introductionsupporting

confidence: 91%

Progesterone and oestrone levels in the gonads and pyloric caeca of the male sea star Asterias rubens: A comparison with the corresponding levels in the female sea star

Voogt

Dieleman

1984

Comparative Biochemistry and Physiology Part A: Physiology

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“…The sensitivity was 015 nmol/1 and the interassay coefficient of variation was 11% (n = 16). A correlation of r = 0-97 (7><0001) was observed for the comparison of the progesterone concentrations throughout the oestrous cycle of 5 cows as estimated by this RIA method with those as estimated by the method described previously (Dieleman & Schoenmakers, 1979;Dieleman eta!, 1986). …”

Section: Methodsmentioning

confidence: 68%

Effects of monoclonal antibody against PMSG administered shortly after the preovulatory LH surge on time and number of ovulations in PMSG/PG-treated cows

Dieleman¹,

Bevers²

1987

Reproduction

137

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Summary. Normally cyclic heifers received 2500 i.u. PMSG i.m. at Day 10 of the oestrous cycle and 15 mg prostaglandin (PG) i.m. 48 h later. From 30 h after PG the LH concentration in the peripheral blood was estimated every hour using a rapid RIA method which allowed the LH concentration to be known within 4 h. Monoclonal antibody against PMSG was injected in the jugular vein of 29 heifers at 4\m=.\8h after the maximum of the preovulatory LH peak; 28 heifers were not treated with anti-PMSG (controls). Peripheral blood concentrations of PMSG, LH, progesterone and oestradiol were compared. Ovaries were collected by ovariectomy at fixed times, 22\p=n-\30h after the LH peak, and numbers were counted of small (2\p=n-\10mm), large ( > 10 mm) and ovulated follicles, and of follicles with a stigma.In anti-PMSG-treated cows, the PMSG concentration fell sharply to non-detectable levels within 2 h of the treatment, indicating that PMSG was neutralized in these cows at the onset of final follicular maturation. In all cows, the concentration of oestradiol showed a significant decrease at about 8 h after the LH peak. After anti-PMSG treatment ovulations took place from 24 until 30 h after the LH peak, whereas in control cows follicles had already ovulated at or before 22 h and ovulations continued until 30 h. At 30 h 90% of the follicles had ovulated in anti-PMSG-treated cows vs 72% in the controls, resulting in 15 and 8 ovulations per cow respectively (P < 0\m=.\05).Also, administration of monoclonal antibody against PMSG synchronized final follicular maturation and shortened the period of multiple ovulations.In conclusion, neutralization of PMSG shortly after the preovulatory LH peak suppresses adverse effects of PMSG on final follicular maturation, leading to an almost 2-fold increase of the ovulation rate.

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“…Progesterone concentrations were determined in plasma samples by the radioimmunoassay method described by Dieleman & Schoenmakers (1979). The intra-and interassay coefficients of variation were 11% and 4%, respectively.…”

Section: Methodsmentioning

confidence: 99%

Resumption of follicular activity in the early post-partum period of dairy cows

Savio

Boland²,

Hynes³

et al. 1990

Reproduction

206

119

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Summary. Lactating Friesian dairy cows (2nd\p=n-\4thparity) which calved in spring (N = 7) or autumn (N = 15) were used. Their ovaries were examined by ultrasound scanning and blood samples were obtained daily for progesterone and oestradiol concentrations from the 5th day after calving until the first post-partum ovulation occurred. Five autumn-calving cows selected at random were bled every 15 min over a 6-h period on 1 day each week for 4 weeks after calving to assess the patterns of LH secretion. Follicular development during the post-partum anoestrous period was characterized by the growth and regression of small ( \ m=l e\ 4 mm) and medium-sized (5\ p=n-\ 9 mm) follicles, until a dominant follicle (> 10 mm) was detected. The first detected dominant follicle ovulated in 14 cows, became cystic in 4 cows (all in autumn), and failed to ovulate in 1 cow. It was not possible to detect a dominant follicle in 3 cows due to scanning difficulties. The post-partum interval to detection of the first dominant follicle (mean \ m=+-\ s.d.) was shorter (P < 0\m=.\05) in autumn (6\m=.\8\ m=+-\ 1\m=.\8days) than in spring (20 \m=+-\10\m=.\1days). However, there was no significant difference between the respective intervals to first ovulation (autumn 27\m=.\4\ m=+-\25\m=.\9and spring 27\m=.\3\ m=+-\18\m=.\9 days). Autumncalved cows which had cysts had longer (P < 0\m=.\001) intervals to first ovulation (58\m=.\2\ m=+-\ 23\m=.\5days) than did normal cows (12\m=.\0\ m=+-\2\m=.\5 days). All cows with cysts had twin ovulations at their first post-partum ovulation. A pulsatile pattern of LH secretion was detected in the first week post-partum and LH pulse frequency was 2\p=n-\3 per 6-h period in Weeks 1 and 2 post partum and increased to 5\p=n-\7 pulses per 6-h period in the presence of a dominant or cystic follicle. Concentrations of progesterone in plasma during post-partum anoestrus were usually low (< 0\m=.\2 ng/ml); oestradiol concentrations were also low (<5pg/ml), but higher values (5\p=n-\110 pg/ml) were observed in cows that had a dominant or a cystic follicle.

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Radioimmunoassays to determine the presence of progesterone and estrone in the starfish Asterias rubens

Cited by 89 publications

References 23 publications

Progesterone and oestrone levels in the gonads and pyloric caeca of the male sea star Asterias rubens: A comparison with the corresponding levels in the female sea star

Progesterone and oestrone levels in the gonads and pyloric caeca of the male sea star Asterias rubens: A comparison with the corresponding levels in the female sea star

Effects of monoclonal antibody against PMSG administered shortly after the preovulatory LH surge on time and number of ovulations in PMSG/PG-treated cows

Resumption of follicular activity in the early post-partum period of dairy cows

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