Solid-phase microextraction in metabolomics

Bojko, Barbara; Reyes‐Garcés, Nathaly; Bessonneau, Vincent; Goryński, Krzysztof; Mousavi, Fatemeh; Souza-Silva, Érica A.; Pawliszyn, Janusz

doi:10.1016/j.trac.2014.07.005

Cited by 135 publications

(95 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Human serum albumin (essentially fatty acid and globulin free) was dissolved in PBS buffer solution (pH 7.4) to reach protein concentrations of 0, 5,10,20,30,40,50,60,70 and 100 g/L, These solutions were then spiked with fatty acids standards to reach the concentration of 3 µg/mL in all solutions. The study was conducted in duplicate.…”

Section: Determination Of Protein Affinity Constantmentioning

confidence: 99%

“…The benefits of SPME for highly complex matrices such as biological samples have been already discussed elswhere. [27][28][29][30][31][32] Headspace SPME (HS--SPME) has been previously reported for determination of short chain volatile fatty acids, including the C 2 --C 7 carbon chain or their ethyl esters from waste water. 33,34 However, extraction of long chain fatty acids in biological and nutrition analysis is a very challenging goal due to their hydrophobicity, perceived abundance as plasticizers, ubiquity in the environment, great tendency to bioconcentrate, vast distribution in conjugated forms in cellular structure, very high affinity to biological proteins such as albumin, and a high risk of matrix effect encounters.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Application of Solid Phase Microextraction for Quantitation of Polyunsaturated Fatty Acids in Biological Fluids

et al. 2014

Self Cite

View full text Add to dashboard Cite

Development of a straightforward strategy for simultaneous quantitative analysis of nonesterified fatty acids (NEFA) species in biofluids is a challenging task because of the extreme complexity of fatty acid distribution in biological matrices. In this study, we present a direct immersion solid phase microextraction method coupled to liquid chromatography--mass spectrometry platform (DI--SPME--HPLC--ESI --MS ) for determination of unconjugated fatty acids (FA) in fish and human plasma. The proposed method was fully validated according to bioanalytical method validation guidelines. The LOD and LOQ were in the range of 0.5--2 and 5--12 ng/ml, respectively, with a linear dynamic range of 100 fold for each compound. Absolute and relative matrix effects were comprehensively evaluated and found to be in the acceptable range of 91--116%. The affinity constant (K a ) of individual FAs to protein albumin was determined to be 9.2×10 4 to 4.3×10 5 M -1 . The plasma protein binding (PPB%) was calculated, and found to be in the range of 98.0--99.7% for different polyunsaturated fatty acids (PUFAs). The PUFAs under study were found at a high concentration range in fish plasma, whereas only a few were within quantification range in control human plasma. The method was successfully applied for monitoring PUFA changes during the operation in plasma samples obtained from patients undergoing cardiac surgery with the use of cardiopulmonary bypass (CPB). The most significant contribution induced by surgery was noticed in the concentration level of α--linolenic acid (18:3, ALA), arachidonic acid (20:4, AA), and docosahexanoic acid (22:6, DHA) soon after administration of CPB in all cases.

show abstract

Section: Determination Of Protein Affinity Constantmentioning

confidence: 99%

mentioning

confidence: 99%

Application of Solid Phase Microextraction for Quantitation of Polyunsaturated Fatty Acids in Biological Fluids

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…As well, due to the nature of SPME extraction, more hydrophobic analytes, which are extracted well, are present at lower free concentration levels compared to low affinity polar components. Thanks to this phenomenon, and by carefully controlling the time of extraction, negative effects associated with the saturation of the coating can be entirely eliminated [104][105][106]. In the study of Vuckovic et al [95] an evaluation of 42 coatings, including commercially available, lab-made, and prototype fibers, provided information regarding the suitability of specific coatings for DI-SPME sample preparation towards global metabolomics projects.…”

Section: Spme In Early Diagnosis Of Highly Prevalent Diseasesmetabolomentioning

confidence: 99%

SPME as a promising tool in translational medicine and drug discovery: From bench to bedside

Goryński

Goryńska

Górska

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

Self Cite

View full text Add to dashboard Cite

“…In addition, to increase method sensitivity and provide wider metabolome coverage, a proper sample preparation strategy, able to obtain the most representative, yet clean extract possible, needs to be used. Solid phase microextraction (SPME), as one of the recently emerging techniques utilized in sample preparation for metabolomics studies, is capable of fulfilling many of the criteria for ideal sample preparation in metabolomic investigations such us non-selectivity, reproducibility, simplicity and possibility for automation (Bojko et al 2014;Vuckovic et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Coupling solid phase microextraction to complementary separation platforms for metabotyping of E. coli metabolome in response to natural antibacterial agents

et al. 2016

Self Cite

View full text Add to dashboard Cite

The final publication is available at Springer via http://dx.doi.org/10.1007Springer via http://dx.doi.org/10. /s11306-016-1111 2 Introduction. Essential oils are known to possess antimicrobial activity; thus, their use has played an important role over the years in medicine and for food preservation purposes.Objective. The effect of clove oil and its major constituents as bactericidal agents on the global metabolic profiling of E. coli bacteria was assessed by means of metabolic alterations, using solid phase microextraction (SPME) as a sample preparation method coupled to complementary analytical platforms.Method. E. Coli cultures treated with clove oil and its major individual components were sampled by HS-SPME-GCxGC-ToF/MS and SPME-UPLC-MS. Full factorial design was applied in order to estimate the most effective antibacterial agent towards E. coli. Central composite design (CCD) and factorial design were applied to investigate parameters influencing metabolite coverage and efficiency by SPME. Results. The metabolic profile, including 500 metabolites identified by LC-MS and 789components detected by GCxGC-ToF/MS, 125 of which were identified as dysregulated metabolites, revealed changes in the metabolome provoked by the antibacterial activity of clove oil, and in particular its major constituent eugenol. Analyses of individual components selected using Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) showed a neat differentiation between control samples in comparison to treated samples in various sets of metabolic pathways.Conclusions. The combination of a sample preparation method capable of providing cleaner extracts coupled to different analytical platforms was successful in uncovering changes in metabolic pathways associated with lipids biodegradation, changes in the TCA cycle, amino acids, and enzyme inhibitors in response to antibacterial treatment.

show abstract

Solid-phase microextraction in metabolomics

Cited by 135 publications

References 79 publications

Application of Solid Phase Microextraction for Quantitation of Polyunsaturated Fatty Acids in Biological Fluids

Application of Solid Phase Microextraction for Quantitation of Polyunsaturated Fatty Acids in Biological Fluids

SPME as a promising tool in translational medicine and drug discovery: From bench to bedside

Coupling solid phase microextraction to complementary separation platforms for metabotyping of E. coli metabolome in response to natural antibacterial agents

Contact Info

Product

Resources

About