SPME as a promising tool in translational medicine and drug discovery: From bench to bedside

Goryński, Krzysztof; Goryńska, Paulina Zofia; Górska, Agnieszka; Harężlak, Tomasz; Jaroch, Alina; Jaroch, Karol; Lendor, Sofia; Skobowiat, Cezary; Bojko, Barbara

doi:10.1016/j.jpba.2016.05.012

Cited by 19 publications

(15 citation statements)

References 104 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because of its simplicity, SPME has been used to monitor the metabolites, ligand–protein binding, toxicity and permeability of drugs, and metabonomics of volatile or semivolatile compounds. Basic theory of this technique is that the solid support, which is hydrophobic and dispersed with extracting phases, is exposed to the test sample for a definite period of time [ 64 ]. Then, the enriched drug molecules in the extraction phase are rapidly and completely separated into the analytical instruments by high temperature or solvent elution methods.…”

Section: Methods To Investigate the Interaction Between Active Herbalmentioning

confidence: 99%

“…Because of the non-depleting extraction mode, SPME is a particular suitable technique for drug-protein binding studies [ 66 ]. The development of biocompatible coating makes SPME can investigate complex biological samples for any binding equilibriums [ 64 , 67 ]. The relative high accuracy and sensitivity, no need to use organic solvents and possibility to automate are the main advantages of SPME.…”

Section: Methods To Investigate the Interaction Between Active Herbalmentioning

confidence: 99%

See 1 more Smart Citation

Study on the interaction between active components from traditional Chinese medicine and plasma proteins

Jiao

Wang

Jiang

et al. 2018

Chemistry Central Journal

View full text Add to dashboard Cite

Traditional Chinese medicine (TCM), as a unique form of natural medicine, has been used in Chinese traditional therapeutic systems over two thousand years. Active components in Chinese herbal medicine are the material basis for the prevention and treatment of diseases. Research on drug-protein binding is one of the important contents in the study of early stage clinical pharmacokinetics of drugs. Plasma protein binding study has far-reaching influence on the pharmacokinetics and pharmacodynamics of drugs and helps to understand the basic rule of drug effects. It is important to study the binding characteristics of the active components in Chinese herbal medicine with plasma proteins for the medical science and modernization of TCM. This review summarizes the common analytical methods which are used to study the active herbal components-protein binding and gives the examples to illustrate their application. Rules and influence factors of the binding between different types of active herbal components and plasma proteins are summarized in the end. Finally, a suggestion on choosing the suitable technique for different types of active herbal components is provided, and the prospect of the drug-protein binding used in the area of TCM research is also discussed.

show abstract

Section: Methods To Investigate the Interaction Between Active Herbalmentioning

confidence: 99%

Section: Methods To Investigate the Interaction Between Active Herbalmentioning

confidence: 99%

Study on the interaction between active components from traditional Chinese medicine and plasma proteins

Jiao

Wang

Jiang

et al. 2018

Chemistry Central Journal

View full text Add to dashboard Cite

show abstract

“…is coupled with chromatography methods for quantitative analysis of compounds in different kinds of matrices. [51][52][53][54] The recent coupling of SPME with ambient MS has opened new opportunities for the field, as significant improvement in quantitative performance were achieved through this development. 55 Theoretically, the fibers and other devices used in SPME-GC or -LC could be used directly for MS analysis.…”

Section: Solid-phase Microextraction (Spme)-spme Is a Sample Preparatmentioning

confidence: 99%

Direct sampling mass spectrometry for clinical analysis

Chiang

Zhang

et al. 2019

Analyst

View full text Add to dashboard Cite

show abstract

“…The device is made of biocompatible materials that prevent adhesion of biological materials to the fibre, while also averting contamination of the sample, which is particularly crucial in living tissue applications. Detailed information regarding the technology and its utilisation in bioanalysis can be found elsewhere [10][11][12][13]. In the present study, the development of a method allowing for in situ and in vivo metabolic profiling of brain tumours is presented.…”

Section: Introductionmentioning

confidence: 99%

A new strategy for brain tumour metabolomic analysis

Goryńska¹,

Chmara²,

Goryński³

et al. 2018

Medical Research Journal

Self Cite

View full text Add to dashboard Cite

Introduction: Nowadays, diagnosis of brain tumours is mainly carried out via neuroimaging techniques. The most widespread methods for routine analysis include computer tomography and magnetic resonance imaging. While such methods are useful to localise tumours, they are unable to offer a conclusive diagnosis of the tumour type. A final diagnosis can only be made via a histological examination of tissue after tumour resection, or, in cases where the location of the tumour is not amenable to resection, after a biopsy of the tumour is carried out. Untargeted metabolite analysis is a relatively new approach to diagnostics, capable of establishing wide characterisation of endogenous metabolites of a given system, a method that can be applied to improve identification of tumour types via biomarker discovery. In this regard, sample collection and preparation can be said to be the most important step in metabolomic studies. Material and methods: In the current study, a solid phase microextraction (SPME) protocol for metabolomics, which has been successfully applied towards metabolite analysis in various biological materials in the last few years, was optimised for brain tumour tissue metabolomic analysis. In the current study, the described approach was applied to human brain tumours. Aiming to incur minimal tissue damage, the probes used for sampling were of diameter ca. 0.2 mm. Aiming to optimise the method towards enhanced recovery of the extracted metabolites, various desorption solvents were tested in an optimisation study. The final protocol was used for analysis of a pilot cohort of patients with glioma and meningioma tumours. Results: The results showed that a protocol where chemical biopsy was performed directly from resected tumour with 7-mm-long coating SPME probe and desorption was done using 0.3 mL of a mixture of acetonitrile and water 80:20 v/v was superior to other tested protocols. The optimised method allowed for successful differentiation between the two types of brain tumours studied: meningioma and glioma. Despite the relatively small cohort group involved in the study, several compounds were tentatively identified as statistically significant metabolites responsible for this differentiation. Conclusions: The presented preliminary data demonstrate a potential of the proposed method as a low invasive diagnostic tool for on-site analysis.

show abstract

SPME as a promising tool in translational medicine and drug discovery: From bench to bedside

Cited by 19 publications

References 104 publications

Study on the interaction between active components from traditional Chinese medicine and plasma proteins

Study on the interaction between active components from traditional Chinese medicine and plasma proteins

Direct sampling mass spectrometry for clinical analysis

A new strategy for brain tumour metabolomic analysis

Contact Info

Product

Resources

About