Solid-phase microextraction: Artefact formation and its avoidance

Verhoeven, H. A.; Beuerle, Till; Schwab, Wilfried

doi:10.1007/bf02490931

Cited by 40 publications

(22 citation statements)

References 16 publications

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“…The fused silica fiber of the SPME device (Supelco) coated with 100 m of polydimethylsiloxane was inserted into the vial through an aluminum cap with a PolyTetraFluoroEthylene/butyl rubber septum, and volatiles were trapped by exposing the fiber to the headspace for 30 min. The SPMEtrapped Arabidopsis volatiles were analyzed by GC-MS as described by Verhoeven et al (1997). The difference in affinity of the SPME fiber to linalool and germacrene A was determined by analyzing a mixture of 1 g/mL linalool and valencene (a sesquiterpene closely resembling germacrene A) in 100 L of water (containing 1% ethanol).…”

Section: Solid-phase Microextractionmentioning

confidence: 99%

Terpenoid Metabolism in Wild-Type and Transgenic Arabidopsis Plants[W]

Aharoni¹,

Giri²,

et al. 2003

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Volatile components, such as terpenoids, are emitted from aerial parts of plants and play a major role in the interaction between plants and their environment. Analysis of the composition and emission pattern of volatiles in the model plant Arabidopsis showed that a range of volatile components are released, primarily from flowers. Most of the volatiles detected were monoterpenes and sesquiterpenes, which in contrast to other volatiles showed a diurnal emission pattern. The active terpenoid metabolism in wild-type Arabidopsis provoked us to conduct an additional set of experiments in which transgenic Arabidopsis overexpressing two different terpene synthases were generated. Leaves of transgenic plants constitutively expressing a dual linalool/nerolidol synthase in the plastids (FaNES1) produced linalool and its glycosylated and hydroxylated derivatives. The sum of glycosylated components was in some of the transgenic lines up to 40-to 60-fold higher than the sum of the corresponding free alcohols. Surprisingly, we also detected the production and emission of nerolidol, albeit at a low level, suggesting that a small pool of its precursor farnesyl diphosphate is present in the plastids. Transgenic lines with strong transgene expression showed growth retardation, possibly as a result of the depletion of isoprenoid precursors in the plastids. In dual-choice assays with Myzus persicae , the FaNES1 -expressing lines significantly repelled the aphids. Overexpression of a typical cytosolic sesquiterpene synthase resulted in the production of only trace amounts of the expected sesquiterpene, suggesting tight control of the cytosolic pool of farnesyl diphosphate, the precursor for sesquiterpenoid biosynthesis. This study further demonstrates the value of Arabidopsis for studies of the biosynthesis and ecological role of terpenoids and provides new insights into their metabolism in wild-type and transgenic plants.

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Section: Solid-phase Microextractionmentioning

confidence: 99%

Terpenoid Metabolism in Wild-Type and Transgenic Arabidopsis Plants[W]

Aharoni¹,

Giri²,

et al. 2003

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“…GC-MS analysis was performed using a Fisons 8060 gas chromatograph directly coupled to an MD 800 mass spectrometer (Fisons, Mainz-Kastel, Germany), as described by Verhoeven et al (1997). Volatiles were extracted as described by Y.T.…”

Section: Volatile Analysis By Gc-msmentioning

confidence: 99%

The light‐hyperresponsive high pigment‐2^dg mutation of tomato: alterations in the fruit metabolome

Bino¹,

Vos²,

et al. 2005

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Summary• Overall metabolic modifications between fruit of light-hyperresponsive highpigment ( hp ) tomato ( Lycopersicon esculentum ) mutant plants and isogenic nonmutant (wt) control plants were compared.• Targeted metabolite analyses, as well as large-scale nontargeted mass spectrometry (MS)-based metabolite profiling, were used to phenotype the differences in fruit metabolite composition.• Targeted high-performance liquid chromatography with photodiode array detection (HPLC-PDA) metabolite analyses showed higher levels of isoprenoids and phenolic compounds in hp-2 dg fruit. Nontargeted GC-MS profiling of red fruits produced 25 volatile compounds that showed a 1.5-fold difference between the genotypes. Analyses of red fruits using HPLC coupled to high-resolution quadrupole time-offlight mass spectrometry (LC-QTOF-MS) in both ESI-positive and ESI-negative mode generated, respectively, 6168 and 5401 mass signals, of which 142 and 303 showed a twofold difference between the genotypes.• hp-2 dg fruits are characterized by overproduction of many metabolites, several of which are known for their antioxidant or photoprotective activities. These metabolites may now be more closely implicated as resources recruited by plants to respond to and manage light stress. The similarity in metabolic alterations in fruits of hp-1 and hp-2 mutant plants helps us to understand how hp mutations affect cellular processes.

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“…The needle of the SPME device (Supelco Inc., Bellefonte, PA) was inserted into the funnel through the foil, and volatiles were trapped by exposing a fused silica fiber coated with 100 M polydimethylsiloxane to the strawberry headspace for 30 min. The SPME-trapped strawberry volatiles were analyzed by gas chromatography-mass spectrometry (GC-MS), as described by Verhoeven et al (1997). Volatile compounds were identified by screening the National Institute of Standards and Technology library for comparable mass spectra and by comparison with authentic reference compounds (Aldrich).…”

Section: Analyses Of Volatiles Released By Ripening Strawberry Fruitsmentioning

confidence: 99%

Identification of the SAAT Gene Involved in Strawberry Flavor Biogenesis by Use of DNA Microarrays

Keizer²,

et al. 2000

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Fruit flavor is a result of a complex mixture of numerous compounds. The formation of these compounds is closely correlated with the metabolic changes occurring during fruit maturation. Here, we describe the use of DNA microarrays and appropriate statistical analyses to dissect a complex developmental process. In doing so, we have identified a novel strawberry alcohol acyltransferase ( SAAT ) gene that plays a crucial role in flavor biogenesis in ripening fruit. Volatile esters are quantitatively and qualitatively the most important compounds providing fruity odors. Biochemical evidence for involvement of the SAAT gene in formation of fruity esters is provided by characterizing the recombinant protein expressed in Escherichia coli . The SAAT enzyme showed maximum activity with aliphatic medium-chain alcohols, whose corresponding esters are major components of strawberry volatiles. The enzyme was capable of utilizing short-and medium-chain, branched, and aromatic acyl-CoA molecules as cosubstrates. The results suggest that the formation of volatile esters in fruit is subject to the availability of acyl-CoA molecules and alcohol substrates and is dictated by the temporal expression pattern of the SAAT gene(s) and substrate specificity of the SAAT enzyme(s).

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Solid-phase microextraction: Artefact formation and its avoidance

Cited by 40 publications

References 16 publications

Terpenoid Metabolism in Wild-Type and Transgenic Arabidopsis Plants[W]

Terpenoid Metabolism in Wild-Type and Transgenic Arabidopsis Plants[W]

The light‐hyperresponsive high pigment‐2^dg mutation of tomato: alterations in the fruit metabolome

Identification of the SAAT Gene Involved in Strawberry Flavor Biogenesis by Use of DNA Microarrays

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Solid-phase microextraction: Artefact formation and its avoidance

Cited by 40 publications

References 16 publications

Terpenoid Metabolism in Wild-Type and Transgenic Arabidopsis Plants[W]

Terpenoid Metabolism in Wild-Type and Transgenic Arabidopsis Plants[W]

The light‐hyperresponsive high pigment‐2dg mutation of tomato: alterations in the fruit metabolome

Identification of the SAAT Gene Involved in Strawberry Flavor Biogenesis by Use of DNA Microarrays

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The light‐hyperresponsive high pigment‐2^dg mutation of tomato: alterations in the fruit metabolome