Solid-phase micro-radioimmunoassay to measure immunoglobulin class-specific antibody to Mycoplasma pulmonis

Taylor, Geraldine

doi:10.1128/iai.24.3.701-706.1979

Cited by 12 publications

(2 citation statements)

References 13 publications

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“…Radioimmunoassay. The radioimmunoassay procedure was similar to that reported by Taylor (31). Polyvinyl vbottom microtiter plates (Costar, Cambridge, Mass.)…”

Section: Methodsmentioning

confidence: 94%

Isotype commitment of B cells and dissemination of the primed state after mucosal stimulation with Mycoplasma pulmonis

Rose¹,

Cebra²

1985

Infect Immun

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Live Mycoplasma pulmonis organisms were used to examine the immune response in the bronchus-associated lymphoid tissue after primary and secondary challenge with M. pulmonis, to study the dissemination of the primed state to distal tissues (i.e., spleen, peripheral blood, and Peyer's patches), and to determine whether the chronic antigenic stimulation accompanying infection influences the isotype potential and commitment of the primed B cells recovered from the various tissues. We have shown that exposure to M. pulmonis by a variety of routes results in a generalized rise in frequency of T-dependent, antigen-sensitive B cells in all lymphoid tissues. The route of secondary exposure to M. pulmonis was found to markedly increase the frequency of M. pulmonis-specific B cells in the bronchus-associated lymphoid tissue relative to that in the Peyer's patches after intraduodenal but not intratracheal challenge. A substantial rise in the number of M. pulmonis-sensitive B cells in the peripheral blood suggests that the dissemination of the primed state, at least in part, is due to B-cell migration via lymph and blood from local sites exposed to M. pulmonis. The majority of T-dependent clones generated by M. pulmonis-specific B cells secrete exclusively immunoglobulin Gl (IgGl). We have demonstrated that the exaggerated IgGl response was not due to the accompanying viable donor T cells in the inoculum. The predominance of IgGl was also demonstrated in clones from the bronchus-associated lymphoid tissue of athymic BALB/c mice that were primed with M. pulmonis. Thus, we can infer that functional T cells are not required for the development of specific B cells with the potential for IgGl expression at the time of in vivo priming. When anti-trinitrophenyl-and anti-M. pulmonis-specific clones were generated in the same splenic fragment cultures stimulated by trinitrophenylated M. pulmonis, only the M. pulmonis-specific clones showed exaggerated IgGl expression. Therefore, we conclude that the exaggerated IgGl response accompanying M. pulmonis infection of euthymic mice seems to be dependent, at least in part, on an intrinsic property of the B cells that develop during this antigenic stimulation. 428 on August 4, 2020 by guest http://iai.asm.org/ Downloaded from

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“…Radioimmunoassay. The radioimmunoassay procedure was similar to that reported by Taylor (31). Polyvinyl vbottom microtiter plates (Costar, Cambridge, Mass.)…”

Section: Methodsmentioning

confidence: 94%

Isotype commitment of B cells and dissemination of the primed state after mucosal stimulation with Mycoplasma pulmonis

Rose¹,

Cebra²

1985

Infect Immun

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“…M. pulmonis antigen was an appropriately diluted suspension of washed organisms that had been grown in mycoplasma medium supplemented with heated fetal calf serum. Antibody titers were expressed in log,( units calculated by reference to a standard serum as described previously (16). Assessment of lung pathology.…”

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confidence: 99%

Class-specific antibody responses to Mycoplasma pulmonis in sera and lungs of infected and vaccinated mice

Taylor¹,

Howard²

1980

Infect Immun

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Class-specific antibodies were measured by a solid-phase microradioimmunoassay in the sera and lung washings of mice after intranasal or intravenous inoculation with live Mycoplasma pulmonis and after systemic, intranasal, or combined vaccination with Formalin-inactivated mycoplasmas. After intranasal or intravenous inoculation with live organisms, serum antibodies were first detected in immunoglobulin M (IgM) followed by IgG2, IgG1, and IgA classes, but significant levels of IgA developed only in those mice inoculated intranasally. The appearance of antibodies in lung washings was later than in serum, but again these were predominantly IgG2 and IgG1. After inoculation with killed organisms, serum antibodies were predominantly IgG1, although IgG2, IgM, and, in intranasally vaccinated mice, IgA were also present. Only IgG1 was detected in lung washings from mice vaccinated systemically, but IgA and IgG2 were present in addition in animals vaccinated intranasally. Immunofluorescence studies indicated that some antibody in lung washings from the latter group of animals was produced locally. A comparison of the levels of various class-specific antibodies and resistance to intranasal challenge suggested that local antibody of any immunoglobulin class is capable of mediating resistance in the lungs to M. pulmonis infection.

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Monoclonal Antibodies as Probes for Antigens of Mycoplasma Pulmonis

Rose

Barile

Cebra

1983

Host Defenses to Intracellular Pathogens

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Solid-phase micro-radioimmunoassay to measure immunoglobulin class-specific antibody to Mycoplasma pulmonis

Cited by 12 publications

References 13 publications

Isotype commitment of B cells and dissemination of the primed state after mucosal stimulation with Mycoplasma pulmonis

Isotype commitment of B cells and dissemination of the primed state after mucosal stimulation with Mycoplasma pulmonis

Class-specific antibody responses to Mycoplasma pulmonis in sera and lungs of infected and vaccinated mice

Monoclonal Antibodies as Probes for Antigens of Mycoplasma Pulmonis

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