Solid-Phase Extraction of Antipyrine Dye for Spectrophotometric Determination of Phenolic Compounds in Water

Morita, Emi; Nakamura, Eiko

doi:10.2116/analsci.27.489

Cited by 24 publications

(14 citation statements)

References 22 publications

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“…[6] Sensitive detection of phenolic compounds has been well established using instrumental analysis such as liquid chromatography. Then the aqueous sample containing phenol was mixed with an aqueous solution of 4-aminoantipyrine and was passed through the membrane with incorporated laccase nanoflowers, causing oxidative coupling of phenol with 4-aminoantipyrine to form an antipyrine dye [8] that has an absorption maximum at 495 nm. As shown in Figure 1, a suspension of laccase-inorganic hybrid nanoflowers, which have a high activity (ca.…”

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confidence: 99%

“…As shown in Figure 1, a suspension of laccase-inorganic hybrid nanoflowers, which have a high activity (ca. Then the aqueous sample containing phenol was mixed with an aqueous solution of 4-aminoantipyrine and was passed through the membrane with incorporated laccase nanoflowers, causing oxidative coupling of phenol with 4-aminoantipyrine to form an antipyrine dye [8] that has an absorption maximum at 495 nm. This procedure thus deposited enzyme nanoflowers with an average size of 4 mm onto the membrane.…”

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confidence: 99%

“…For comparison, free laccase solution was injected into the filter and dried inside the filter by the same procedure, allowing the dried protein to be adsorbed onto the membrane. [8] The main advantages of this method are that the analytical procedure only requires five minutes, and the laccasecatalyzed coupling reaction is specific to phenolic compounds, [10] which is very suitable for on-site and fast detection of phenols in environmental water. Then, 300 mL of the mixture was injected into the nanoflower-coated membrane filter and kept in the filter chamber for five minutes.…”

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confidence: 99%

“…The high activity of laccase nanoflowers allowed the fast oxidative coupling of phenol with 4-aminoantipyrine to form antipyrine dyes. [8] Afterwards, the solution was pushed out and collected for measurement by naked-eye visualization or by using a UV/Vis spectrophotometer at 495 nm. Figure 3 b shows analysis of the samples with different concentrations (from 50 to 0.4 mg mL À1 in 50 mm pH 6.0 phosphate buffer).…”

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Rapid Detection of Phenol Using a Membrane Containing Laccase Nanoflowers

Zhu

Gong

Zhang

et al. 2013

Chemistry An Asian Journal

130

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Dedicated to Professor Chunli Bai, President of the Chinese Academy of Sciences, in celebration of his 60th birthday.With the rapid development of nanoscience and nanotechnology, [1a] nanostructured biocatalysts that take the advantage of nanomaterials in terms of both functional and structural availability have offered new opportunities for improving biological functions of enzymes and expanding applications in areas such as biosensors, bioanalytical devices, and industrial biocatalysis. [1] Recently, we reported a method of preparing protein-inorganic hybrid nanostructures with flower-like shapes, [2] which have shown much greater activities than free enzymes and most of the reported immobilized enzymes. [3] To bring this appealing catalyst into practical use, however, an effective accommodation of these high-performance enzyme catalysts is required. One way is to weakly attach these enzyme nanoflowers to porous materials by physical adsorption. Recently, Krieg et al. [4] reported the fabrication of a supramolecular membrane by noncovalent modification of a commercial membrane, which suggests the possibility of fabricating functional filtration membranes by a simple post-modification procedure, thus enabling many new and interesting applications. It thus came to our mind to fabricate a membrane incorporating enzyme nanoflowers for the rapid detection of hazardous compounds through visualization of the catalyzed product. Owing to their high toxicity even at a low concentration, [5] phenols are listed as major toxic pollutants by the Environmental Protection Agency of the USA and other countries. [6] Sensitive detection of phenolic compounds has been well established using instrumental analysis such as liquid chromatography. [7] However, these methods usually require sophisticated instrumentation and a multistep procedure, making them less convenient for rapid and on-site detection.The present study started by the fabrication of an enzyme nanoflower incorporated into a membrane. As shown in Figure 1, a suspension of laccase-inorganic hybrid nanoflowers, which have a high activity (ca. 200 % that of free laccase) for phenol oxidization, as we observed previously, [2] was injected into a commercial disposable syringe filter equipped with a cellulose acetate membrane (pore size 0.2 mm). This procedure thus deposited enzyme nanoflowers with an average size of 4 mm onto the membrane. Then the aqueous sample containing phenol was mixed with an aqueous solution of 4-aminoantipyrine and was passed through the membrane with incorporated laccase nanoflowers, causing oxidative coupling of phenol with 4-aminoantipyrine to form an antipyrine dye [8] that has an absorption maximum at 495 nm. This procedure allowed rapid analysis by a UV/ Vis spectrophotometer or by the naked eye. Finally, pure water was injected into the filter to remove unreacted reagents and the reaction products, followed by drying the membrane in air for the next use.For the preparation of laccase-copper phosphate nanoflowers, typically, 0.8 mm aqueous Cu...

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confidence: 99%

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Rapid Detection of Phenol Using a Membrane Containing Laccase Nanoflowers

Zhu

Gong

Zhang

et al. 2013

Chemistry An Asian Journal

130

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“…In environmental samples, the complexity of co-existing materials and trace level concentrations of target analytes were expected, so solid phase extraction (SPE) is important to extract target compounds from samples. 26,27 In this study, a simultaneous detection method by LC-MS/MS and SPE process was applied for caffeine and its demethylated metabolites in environmental samples.…”

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Determination of Caffeine and Its Metabolites in Wastewater Treatment Plants Using Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry

Echigo

Itoh

2018

ANAL. SCI.

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For caffeine and its seven major metabolites (i.e., theobromine, theophylline, paraxanthine, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine, and xanthine), an optimized analytical method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for their detection in wastewater samples was developed in this study. Extraction of these compounds (recoveries ranged from 60.3 to 83.2%) was made possible by combining universal polymeric reversedphase cartridge and polymeric strong cation exchange cartridge. This method was applied to the determination of caffeine and its metabolites in the influent and effluent of an anaerobic-anoxic-oxic (A2O) process. In the A2O influent, caffeine and its metabolites (except xanthine) ranged from 1.39 to 5.45 μg/L, and their concentrations in the A2O effluent ranged from 10.2 to 171.3 ng/L. The mass load of caffeine was 14.9 g/day/1000 inhabitants, considering the population served by the target wastewater treatment plant (WWTP). The concentration of caffeine derivatives in wastewater influent is a tool for estimating the population size in the area served by WWTPs.

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