Solid-gas reactions for nitroxyl (HNO) generation in the gas phase

Discovery of endogenous nitroxyl as a new redox player in Arabidopsis thaliana

“…1f ). The method has demonstrated specificity for HNO, with no interference or spurious signal arising from •NO, O 2 , NO 2 − and other RNS 11 , 13 , 31 , 32 . The calibration curve measures current responses at a potential of 0.8 V with the addition of freshly prepared AS, the HNO donor (Merck) 3 , 14 , 33 and aqueous solutions of 30–900 nM nitroxyl (Extended Data Fig.…”

“…These studies are the first to confirm endogenous HNO production in living cells and point toward a novel regulatory role of the molecule in the ethylene signalling pathway in plants. Added to our previous findings on HNO chemistry 4 , 11 , 13 , 15 , our revelation of HNO’s effects on plant •NO signalling and metabolism mandate the inclusion of nitroxyl in the group of gasotransmitters now composed of •NO, CO and H 2 S. The ubiquitous bioavailability of the HNO molecule provided by non-enzymatic •NO/HNO interconversion allows it to support or compete with •NO signalling, depending on the local redox environment. Nitroxyl’s contribution to the reactive species interactome engaged in cell signalling requires re-evaluation of the consensus on •NO biology.…”

“…Our previously described method is based on a three-electrode system consisting of a platinum counter electrode, an Ag/AgCl reference electrode and a gold working electrode modified with a cobalt porphyrin covalently attached via a thiol moiety 11 , 12 . Note that this method targets the HNO molecule; no interference or spurious signal arises from the presence of •NO, O 2 , NO 2 − or other RNS 11 – 13 .…”

Update on Endogenous HNO Production: An Exploration of Unanswered Questions and Challenges

“…[1][2][3][4][5][6][7] The available information has revealed enormous potential for its application in medicine. [6,[8][9][10] HNO initially emerged as a biologically active component in studies involving cyanamide (H 2 NCN), a drug used to treat alcoholism. [11][12][13] Further investigations revealed that the release of HNO via cyanamide induced vasorelaxation in the rabbit thoracic aorta.…”

“…Over the last decades, extensive studies have been conducted on azanone due to its high biological significance [1–7] . The available information has revealed enormous potential for its application in medicine [6,8–10] . HNO initially emerged as a biologically active component in studies involving cyanamide (H 2 NCN), a drug used to treat alcoholism [11–13] .…”