Sol−Gel Immunosorbents Doped with Polyclonal Antibodies for the Selective Extraction of Malathion and Triazines from Aqueous Samples

Vera-Avila, Luz E.; Vazquez-Lira, Juan C.; Llasera, Martha Garcia De; Covarrubias, Rosario

doi:10.1021/es048000c

Cited by 24 publications

(7 citation statements)

References 23 publications

(44 reference statements)

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“…Methanol is a mild elution medium without causing irreversible denaturation in order to allow the re-usage of these prepared IACs. What's more, methanol performed as the elution solvent is easy to concentrate without extra salt ions effect in MS/MS analysis, which is superior to acid or other buffer solutions (Vera-Avila, Vazquez-Lira, Garcia, & Covarrubia, 2005).…”

Section: Performance and Optimization Of Iac Conditionsmentioning

confidence: 99%

Broad-specific monoclonal antibody based IACs purification coupled UPLC-MS/MS method for T-2 and HT-2 toxin determination in maize and cherry samples

Lin

Wang

et al. 2020

Food and Agricultural Immunology

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In this work, broad-specific monoclonal antibody based immunoaffinity columns (IACs) coupled to ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) for purification and determination of T-2 and HT-2 toxin in maize and cherry samples. Based on broad-specific anti-T-2 monoclonal antibody, the prepared IACs could be used for both T-2 and HT-2 purification with cross reactivity values at 100% for T-2 and 108% for HT-2. Column capacity was 3.14 μg mL −1 gel for T-2 toxin, and 3.20 μg mL −1 gel for HT-2 toxin. After 10 cycles of usage at intervals of 2 days, column capacity decreased to 0.85 μg mL −1 gel for T-2 and 1.22 μg mL −1 gel for HT-2 toxin. The developed method exhibited high sensitive results with limit of detection (LOD) lower than 0.35 μg kg −1 and 1.42 μg kg −1 .

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Section: Performance and Optimization Of Iac Conditionsmentioning

confidence: 99%

Broad-specific monoclonal antibody based IACs purification coupled UPLC-MS/MS method for T-2 and HT-2 toxin determination in maize and cherry samples

Lin

Wang

et al. 2020

Food and Agricultural Immunology

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“…This method has been successfully applied by many laboratories, including ours, for purification of many compounds in the analysis of a variety of environmental, food, and biological samples. For review see [11][12][13][32][33][34][35][36][37][38][39][40][41][42][43][44]) and references therein.…”

Section: Introductionmentioning

confidence: 99%

Development of Immunochemical Methods for Purification and Detection of the Steroid Drug Medroxyprogesterone Acetate

Bronshtein¹,

Krol²,

Schlesinger³

et al. 2012

JEP

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An immunochemical sol-gel-based immunoaffinity purification (IAP) method for purification and detection of the progestin drug medroxyprogesterone acetate (MPA) was developed. A polyclonal antibody (Ab) for MPA was generated, and two competitive (indirect and direct) sensitive enzyme-linked immunosorbent assays (ELISAs) for its detection were developed and implemented to determine the recovery and efficiency of the sol-gel based IAP method. The detection limits of the assays were 1.4 ± 0.2 ng·mL^-1 (n = 4) and 4.0 ± 0.4 ng·mL^-1 (n = 25) for the indirect and direct ELISAs, respectively. The Abs did not exhibit cross-reactivity with any other progestin or steroid hormone, with the exception of megestrol acetate, with which the Ab exhibited 76% cross-reactivity. The sol-gel IAP method successfully eliminated serum interference to a degree that enabled ELISA analysis of spiked serum samples. This method was also found fully compatible with subsequent chemical analytical methods, such as liquid chromatography followed by mass spectrometry (LC-MS/MS). The approaches developed in this study form a basis for analysis of MPA in biological samples and may be further used to study population exposure to MPA and to monitor MPA contamination in water samples

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“…Agarosebased IASs for the enrichment of SUs from soil were described for triasulfuron (22) and chlorimuron-ethyl (23). The development of sol-gel glass supports and their application to environmental samples such as surface water, seawater, and rainwater so far were reported only for polycyclic aromatic hydrocarbons (24), triazine herbicides (25), malathion (26), and isoproturon (27). Corresponding supports for food analysis were described for isoproturon (27), 1-nitropyrene (28), and bisphenol A (29,30).…”

Section: Introductionmentioning

confidence: 99%

Determination of Sulfonylurea Herbicides in Water and Food Samples Using Sol−Gel Glass-Based Immunoaffinity Extraction and Liquid Chromatography−Ultraviolet/Diode Array Detection or Liquid Chromatography-Tandem Mass Spectrometry

Degelmann

Egger

Jürling

et al. 2006

J. Agric. Food Chem.

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Immunoaffinity supports (IAS) were prepared using broad specific polyclonal anti-sulfonylurea (SU) antibodies immobilized in sol-gel glass. Two different kinds of supports were applied, crushed sol-gel monoliths and sol-gel-coated highly porous silica particles. Both were used for the quantitative enrichment of SUs in natural water and food samples followed by high-performance liquid chromatography-ultraviolet/diode array detection (HPLC-UV/DAD) and tandem mass spectrometry (LC-MS/MS), respectively. Loading, washing, and elution conditions of IAS were optimized. The capacity of supports was determined for 30 SUs and compared with the cross-reactivity pattern of the direct competitive enzyme-linked immunosorbent assay. The capacities correlated well with the affinity to individual SU compounds. Even analytes to which the polyclonal antibodies showed only a lower cross-reactivity could be enriched to a certain degree, if a sufficient capacity of IAS was provided. The IAS could be reused at least 10 times without a loss of effectiveness. Recovery of 16 selected SUs extracted from spiked water and food samples was dependent on the affinity of both immobilized antibodies to single compounds and matrix interferences. In water, 13 SUs showed recoveries higher than 80% when immunoaffinity extraction was used in combination with LC-UV/DAD. On the basis of the enrichment of 200 mL of aqueous sample, corresponding limit of detection (LOD) values ranged between 20 and 100 ng/L. The recoveries of 10 SUs, which were extracted from 10 g of potato spiked at a 10 microg/kg level, were higher than 75%. For grain samples, recoveries were at the same order for at least five SU herbicides. The LOD of LC-MS/MS measurements was about 1 order of magnitude higher, i.e., gave LODs between 1.1 and 6.9 microg/kg of food sample, depending on the compound and extraction procedure. These LODs provide evidence that the main advantage of the prepared IAS is their high selectivity for group specific recognition of SUs as compared to other nonspecific solid phase extraction materials.

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Sol−Gel Immunosorbents Doped with Polyclonal Antibodies for the Selective Extraction of Malathion and Triazines from Aqueous Samples

Cited by 24 publications

References 23 publications

Broad-specific monoclonal antibody based IACs purification coupled UPLC-MS/MS method for T-2 and HT-2 toxin determination in maize and cherry samples

Broad-specific monoclonal antibody based IACs purification coupled UPLC-MS/MS method for T-2 and HT-2 toxin determination in maize and cherry samples

Development of Immunochemical Methods for Purification and Detection of the Steroid Drug Medroxyprogesterone Acetate

Determination of Sulfonylurea Herbicides in Water and Food Samples Using Sol−Gel Glass-Based Immunoaffinity Extraction and Liquid Chromatography−Ultraviolet/Diode Array Detection or Liquid Chromatography-Tandem Mass Spectrometry

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