Soilless Cultures for Pharmaceutical Use and Biodiversity Conservation

Cristea, Vasile; Deliu, Constantin; Oltean, B.; Butiuc-Keul, Anca; Albu, Cristian; Radu, Gabriel Lucian

doi:10.17660/actahortic.2009.843.19

Cited by 15 publications

(13 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polyphenol quantitation in the tested extracts was achieved as described in [21]. The HPLC-DAD-MS measurements were performed using a complete HPLC SHIMADZU system, Nucleosil 100 -3.5 C18 column, KROMASIL, 100 × 2.1 mm.…”

Section: High-performance Liquid Chromatography-mass Spectrometry (Hpmentioning

confidence: 99%

Biological activities of the hydro-alcoholic and aqueous extracts of <i>Achillea fragrantissima</i> (Forssk.) grown in Jordan

Hammad¹,

Matar²,

Liţescu³

et al. 2014

View full text Add to dashboard Cite

Aqueous and hydro-alcoholic extracts of Achillea fragrantissima L. (Asteraceae) grown in Jordan were screened for their antioxidant, antimicrobial, antiplatelet, anti-proliferative and acetylcholinesterase (AChE) inhibition efficacy. Total phenols and flavonoids were determined colorimetrically. The radical scavenging activities were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging activity assays. High performance liquid chromatography-mass spectrometry (HPLC-MS) analysis resulted in the identification of 7 phenolic compounds in the hydro-alcoholic extract and 4 compounds in the aqueous extract; quercetin 3-β-D-glucoside was the main component for both extracts. Antimicrobial activities were determined by antimicrobial susceptibility testing such as agar well-diffusion method, minimum inhibition concentration and minimum bactericidal concentration. Gram positive bacteria showed sensitivity to hydro-alcoholic extract in the agar-well diffusion test. No significant activity was observed against gram negative bacteria and Candida albicans. Hydro-alcoholic extract had a bactericidal activity against Streptococcus pneumoniae and Bacillus cereus at high concentrations (MIC 12.5 mg/ml) rather than inhibitory effect. In vitro antiplatelet activity was tested on human whole blood using an electrical impedance method. At concentrations (50, 100, and 200 μg/ml), no effect on platelet aggregation was noticed. Anti-proliferative activity was investigated using the MTT assay. At concentrations up to 200 μg/ml, extracts did not possess cytotoxic activity against the MCF-7 cells. Acetylcholinesterase (AChE) inhibitory capacity of A. fragrantissima extracts was tested using TLC assay method, and neither aqueous, nor hydroalcoholic extracts showed AChE inhibition. The present investigation supported the traditional use of A. fragrantissima in the Jordanian folk medicine as an antimicrobial active representative of the genus Achillea. A. fragrantissima extracts should be further studied for their potential use in preventing/treating diseases in which oxidative stress is a part of the pathophysiology.

show abstract

Section: High-performance Liquid Chromatography-mass Spectrometry (Hpmentioning

confidence: 99%

Biological activities of the hydro-alcoholic and aqueous extracts of <i>Achillea fragrantissima</i> (Forssk.) grown in Jordan

Hammad¹,

Matar²,

Liţescu³

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The experiments to develop a method for measuring polyphenols were based on the HPLC method published by Cristea et al (2009) for the measurement of these compounds. The HPLC-DAD-MS measurements were performed using a complete HPLC SHIMADZU system, Nucleosil 100-3.5 C18 column, KROMASIL, 100 × 2.1 mm.…”

Section: High-performance Liquid Chromatography-mass Spectrometry (Hpmentioning

confidence: 99%

“…Nitrogen was used as the nebulising and drying gas. The SCAN (m/z 50 to 800) mode was used for identification of hesperidin and the SIM mode was used when a search for some particular ions should be done (Cristea et al, 2009). …”

Section: High-performance Liquid Chromatography-mass Spectrometry (Hpmentioning

confidence: 99%

Biological activities of the hydro-alchoholic and aqueous extracts of Achillea biebersteinii Afan. (Asteraceae) grown in Jordan

Hammad¹

2013

Afr. J. Pharm. Pharmacol.

View full text Add to dashboard Cite

Aqueous and hydro-alcoholic extracts of Achillea biebersteinii Afan. (Asteraceae) grown in Jordan were screened for their antioxidant, antibacterial and antiplatelet efficacy. Total phenols and flavonoids were determined colorimetrically. The radical scavenging activities were evaluated using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging activity assays. Antimicrobial activities were determined by the disc-diffusion method, and the minimum inhibition concentration and the minimum bactericidal concentration tests. Gram positive bacteria presented sensitivity to the hydro-alcoholic extract in the disk diffusion test. No significant activity was observed against Gram negative bacteria and Candida albicans. Hydro-alcoholic extract had a bactericidal activity against Streptococcus pneumoniae, Bacillus cereus, Enterococcus faecalis and Klebsiella pneumoniae rather than inhibitory effect. In vitro antiplatelet activity was determined with human whole blood using an electrical impedance method. At concentrations (50, 100, and 200 μg/ml), the extracts showed a non-dose dependent enhancement of platelet aggregation induced by adenosine diphosphate (ADP) (51.82, 52.73 and 51.82%, respectively) and collagen (0.00, 11.76 and 23.53%, respectively). High performance liquid chromatography-mass spectrometry (HPLC-MS) analysis resulted in the identification of 8 phenolic compounds; quercetin 3-β-D-glucoside was the main component.

show abstract

“…*p < 0.05 and ***p < 0.001 compared to control untreated animals, as determined by aNoVa followed by dunnett's post-test. LCMS-2010 detector, equipped with an ESI interface (Cristea et al 2009). The analyses were performed at 20 °C (column temperature) for the period of 70 min, and the injection volume was 20 μL.…”

Section: Preparation Of the Arum Spp Extracts And Hplc-ms Determinationmentioning

confidence: 99%

In vitroandin vivocomparison of the biological activities of two traditionally and widely usedArumspecies from Jordan:Arum dioscoridisSibth & Sm. andArum palaestinumBoiss.

Afifi

Kasabri

Liţescu

et al. 2015

Natural Product Research

View full text Add to dashboard Cite

Arum dioscoridis and A. palaestinum (Araceae) are indigenous plant species in Jordan. HPLC-MS analysis of A. dioscoridis revealed the presence of apigenin, luteolin, quercetin, quercetin-3-O-β-glucoside, vitexin, isoorientin, esculin, and caffeic and ferulic acids. Both Arum spp., influenced gastrointestinal carbohydrate and lipid digestion and absorption. Orlistat inhibited dose dependently and highly substantially pancreatic lipase (PL) in vitro. Similar to orlistat, Arum species aqueous extracts (AEs), apigenin, caffeic acid and esculin exhibited a concentration related PL inhibition. Comparable to acarbose, dual inhibition of α-amylase/α-glucosidase was observed for both Arum species. Like guar gum, A. dioscoridis AE minimised substantially area under 24 h glucose curve. Acute starch-induced postprandial hyperglycaemia in overnight fasting rats was highly significantly (p < 0.001) decreased by A. dioscoridis AE. A. palaestinum could not perform effectively in either starch- or glucose-fed fasting rats. No antiproliferative effects against colorectal cancer cell lines HT29, HCT116 and SW620 were detected for tested Arum spp.

show abstract

Soilless Cultures for Pharmaceutical Use and Biodiversity Conservation

Cited by 15 publications

References 8 publications

Biological activities of the hydro-alcoholic and aqueous extracts of <i>Achillea fragrantissima</i> (Forssk.) grown in Jordan

Biological activities of the hydro-alcoholic and aqueous extracts of <i>Achillea fragrantissima</i> (Forssk.) grown in Jordan

Biological activities of the hydro-alchoholic and aqueous extracts of Achillea biebersteinii Afan. (Asteraceae) grown in Jordan

In vitroandin vivocomparison of the biological activities of two traditionally and widely usedArumspecies from Jordan:Arum dioscoridisSibth & Sm. andArum palaestinumBoiss.

Contact Info

Product

Resources

About

Soilless Cultures for Pharmaceutical Use and Biodiversity Conservation

Cited by 15 publications

References 8 publications

Biological activities of the hydro-alcoholic and aqueous extracts of &lt;i&gt;Achillea fragrantissima&lt;/i&gt; (Forssk.) grown in Jordan

Biological activities of the hydro-alcoholic and aqueous extracts of &lt;i&gt;Achillea fragrantissima&lt;/i&gt; (Forssk.) grown in Jordan

Biological activities of the hydro-alchoholic and aqueous extracts of Achillea biebersteinii Afan. (Asteraceae) grown in Jordan

In vitroandin vivocomparison of the biological activities of two traditionally and widely usedArumspecies from Jordan:Arum dioscoridisSibth & Sm. andArum palaestinumBoiss.

Contact Info

Product

Resources

About

Biological activities of the hydro-alcoholic and aqueous extracts of <i>Achillea fragrantissima</i> (Forssk.) grown in Jordan

Biological activities of the hydro-alcoholic and aqueous extracts of <i>Achillea fragrantissima</i> (Forssk.) grown in Jordan