Soil polymicrobial biofilms resistance increasing using Biogeosystem Technique transcendental environmental services

Abstract

<p>Soil organic matter biodegradation is an agent of the soil fertility and passivation of the hazardous substances including heavy metals. Bacteria within specific habitats, be it the mouth, tonsils, intestines, gut, vagina, or soil are not a faceless mixture of the once acquired participants, but the structurally strictly ordered polymicrobial communities where each participant takes its specific functional place. The conditions for polymicrobial biofilms in the soil are important.</p><p>The aim was tracking down the structural organization and adherence to soil particles of the polymicrobial communities and biofilms, responsible for biodegradation.  Polymicrobial communities and biofilms can be used as a starter, indicator, and control tools for the targeted soil and landscape improvements. Multiple skills in identification, characterizing and monitoring of functional activity of polymicrobial biofilms in the human body and gut were developed in the laboratory of polymicrobial infections and biofilms of the Charité hospital over the past 30 years. The biofilms do not occur in all systems and at any time in relevant amounts. The biochemical activity of the microorganisms till now is investigated solely in pure cultures. As soon as more than three different taxa are involved, the cultivation of the target microorganisms got problematic. The mapping of biofilms by the FISH method is promising for the following objectives in the soil system:</p><p>- identification of the structured polymicrobial biofilms for optimal composting, soil fertility, and a healthy environment;</p><p>- revealing modelling the polymicrobial starter of soil fertility;</p><p>- polymicrobial biofilms activity ensuring via control of the soil architecture, soil moisture and aeration;</p><p>- aerobe/anaerobe conditioning, pH, humic acids, and organic and mineral fertilizers, amelioration and remediation additives;</p><p>- testing of the substrate-bound polymicrobial biofilms as a starter for the shaping of different lands and agricultures.</p><p>Development of the soil-microbiological theoretical and technical fundamentals for the long-term soil improvement and environmentally safe organic wastes recycling and heavy metal passivation into the synthesized soil multilevel aggregate system under minimal intra-soil moistening and appropriate intra-soil mineral and organic matter, and waste application using Biogeosystem Technique (BGT*) transcendental environmental services.</p><p>The soil-microbiological theoretical and technical fundamentals are useful for long-term soil improvement and environmentally safe and eсonomically efficient organic wastes recycling into the synthesized soil aggregate system. The transcendental intra-soil aggregate system construction, the pulse intra-soil continuously-discrete watering, dispersed intra-soil matter application are decisive for higher soil microbial activity and target polymicrobial infections and biofilms transformation into the environmentally safe fertile substances.</p><p>Comparative characterization of the polymicrobial community dynamics in colon and soils will help to promote the function of polymicrobial biofilms in the soil as a specific starter. The BGT* methodology is capable to ensure the soil fertility, improve the soil polymicrobial biofilms resistance, and provide the soil and human health.</p><p>The research was financially supported by the RFBR, projects no. 18-29-25071 and 19-29-05265.</p>