Soil Microbe Diversity and Root Exudates as Important Aspects of Rhizosphere Ecosystem

Bashir, Owais; Khan, Kamran; Hakeem, Khalid Rehman; Mir, Niyaz A.; Rather, G. H.; Mohi-ud-din, Rehana

doi:10.1007/978-3-319-29573-2_15

Cited by 24 publications

(25 citation statements)

References 146 publications

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“…Bacterial diversity in beet rhizosphere. Differences in rhizosphere soil physicochemical properties observed in our study, may be due to greater nutritional demands of the two beet genotypes (TN, Na) or varying exudates composition (OC), as it was found that rhizodeposition is the primary organic carbon source in the rhizosphere 30 . Alternatively, they might be caused by changes in microbial activity resulting from microbial metabolic activity or interaction between microorganisms 31,32 .…”

Section: Discussionmentioning

confidence: 53%

Choosing source of microorganisms and processing technology for next generation beet bioinoculant

Szymańska

Sikora

Hrynkiewicz

et al. 2021

Sci Rep

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The increase of human population and associated increasing demand for agricultural products lead to soil over-exploitation. Biofertilizers based on lyophilized plant material containing living plant growth-promoting microorganisms (PGPM) could be an alternative to conventional fertilizers that fits into sustainable agricultural technologies ideas. We aimed to: (1) assess the diversity of endophytic bacteria in sugar and sea beet roots and (2) determine the influence of osmoprotectants (trehalose and ectoine) addition during lyophilization on bacterial density, viability and salt tolerance. Microbiome diversity was assessed based on 16S rRNA amplicons sequencing, bacterial density and salt tolerance was evaluated in cultures, while bacterial viability was calculated by using fluorescence microscopy and flow cytometry. Here we show that plant genotype shapes its endophytic microbiome diversity and determines rhizosphere soil properties. Sea beet endophytic microbiome, consisting of genera characteristic for extreme environments, is more diverse and salt resistant than its crop relative. Supplementing osmoprotectants during root tissue lyophilization exerts a positive effect on bacterial community salt stress tolerance, viability and density. Trehalose improves the above-mentioned parameters more effectively than ectoine, moreover its use is economically advantageous, thus it may be used to formulate improved biofertilizers.

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Section: Discussionmentioning

confidence: 53%

Choosing source of microorganisms and processing technology for next generation beet bioinoculant

Szymańska

Sikora

Hrynkiewicz

et al. 2021

Sci Rep

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“…Matters detected by soil metabolomic analysis consist of these metabolic substances. Plant root exudates have the capability to enhance stress tolerance of plants and promote growth of soil microbes (Bashir et al 2016). According to relevant findings, corn root exudates contain asparaginic acid, tyrosine, starch and flavonoids (Kuang et al 2003;Carvalhais et al 2011;Zhu et al 2016;Luo et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Analysis of Rhizosphere Soil Fertility in Maize (Zea mays) at Milking Stage

Luan¹

2021

IJAB

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In this study rhizosphere soil of cornat a milk stage was collected to investigate characteristic metabolites and their potential functions. Total nitrogen, organic matters, ammonium nitrogen, pH values, available phosphorus and potassium were determined by semimicro-Kjeldahl method, potassium dichromate (external heating) method, indophenol blue colorimetric method, potentiometry, NaHCO3leaching-molybdenum-antimony colorimetric method and NH4OAc leaching-flame spectrometry, respectively. In addition, UPLC-Q/TOF-MS was adopted for non-targeted metabolomicanalysis. As revealed by results, the total nitrogen contents in soils collected from Dongchuang (i.e., DCMRS for short)0.67±0.14mg/kg lower than from Fumin (i.e., FMMRS for short); moreover, both DCMRS and FMMRS were acid soils. DCMRS contains higher levels of AN (Ammonium nitrogen), SOC (Soil organic carbon), and AP (Available phosphorus) than FMMRS. The amount of TN (Total nitrogen) contained in FMMRS soil was2.410±0.422mg/kg, which is higher than DCMRS. All data derived from UPLC-Q/TOF-MS met the corresponding requirements for further analysis. Metabolites such as 2-methyl-1-propylamine, gamma-butyrolactone and 3-methyl-1-butylamine were detected in DCMRS and FMMRS samples. Several pathways were included, such as lipid metabolism, xenobioticsbiodegradation and metabolism, terpenoids and polyketides, and amino acid metabolism. Through comparison of FMMRS and DCMRS, metabolic pathways associated with nitrogen, carbon, and antibiotic metabolism including iron transport were significantly different between them. Taken together, FMMRS is more fertile, less acidic, and higher in nitrogen than DCMRS.© 2021 Friends Science Publishers

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“…These results are confirmed by the studies of Koohakan et al [40], who were detecting more bacteria and fungi on roots than in nutrient solution, in various types of tomato soilless production systems. The higher number of microorganisms in the root zone is the result of the passive and active leakage of root exudates, which serve as nutrient source for these organisms [41,42].…”

Section: Microbial Analyses Of Drainage Watersmentioning

confidence: 99%

Parameters of Drainage Waters Collected during Soilless Tomato Cultivation in Mineral and Organic Substrates

et al. 2020

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The aim was to determine the suitability of various substrates for application in a closed system of soilless tomato cultivation, based on the potential fitness of drainage waters from these substrates for recirculation. Four substrates were used: rockwool, coir substrate, lignite substrate (Carbomat) and biodegradable organic substrate (Biopot). Tomato plants grown in these substrates were fertilized with the same amount of nutrient solution, containing the same concentration of nutrients. The characteristics of drainage water from these substrates were analyzed during cultivation. The highest amount of drainage water was collected from the lignite substrate Carbomat. However, these leachates showed good properties for further recirculation: low electro conductivity and turbidity, high nutrient content, moderate microbial load with high population of Trichoderma fungi, and being beneficial for plant growth. Moreover, Carbomat produced the highest tomato yield compared to other substrates. This indicates that this organic substrate is an efficient alternative to rockwool and its drainage water may be reused in a recirculation system. On the contrary, the drainage water from the Biopot substrate showed the worst qualities: high pH and low EC, low concentration of nitrate nitrogen and phosphorus, very high turbidity and a high number of microorganisms. These parameters do not qualify Biopot drainage waters for reuse.

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Soil Microbe Diversity and Root Exudates as Important Aspects of Rhizosphere Ecosystem

Cited by 24 publications

References 146 publications

Choosing source of microorganisms and processing technology for next generation beet bioinoculant

Choosing source of microorganisms and processing technology for next generation beet bioinoculant

Metabolomic Analysis of Rhizosphere Soil Fertility in Maize (Zea mays) at Milking Stage

Parameters of Drainage Waters Collected during Soilless Tomato Cultivation in Mineral and Organic Substrates

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