Soft tissue reactions to bioactive glass 13‐93 combined with chitosan

Asikainen, Antti J; Hagström, Jaana; Sorsa, Timo; Noponen, Jukka; Kellomäki, Minna; Juuti, Hanne; Lindqvist, Christian; Hietanen, Jarkko; Suuronen, Riitta

doi:10.1002/jbm.a.31225

Cited by 17 publications

(13 citation statements)

References 35 publications

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“…25–27 There are many studies, which showed minimal inflammatory response of chitosan, 28–29 but there also exists many studies which show that chitosan could lead to high degree of inflammatory infiltrates 30–31 and that reaction could last for long periods. 32 But in our case, for chitosan-TPP and chitosan-TPP-BMP-7 microparticles fabricated by coacervation technique, we observed minor inflammatory response at week 6, which reduced by 12 weeks period. We observed that the granulation tissue is separated from the biomaterial by the cellular components of foreign body reaction where one or two layer of monocytes, macrophages and foreign body giant cells were present.…”

Section: Discussioncontrasting

confidence: 62%

Bone regeneration using injectable BMP-7 loaded chitosan microparticles in rat femoral defect

Mantripragada

Jayasuriya

2016

Materials Science and Engineering: C

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Injectable chitosan microparticles were prepared using a simple coacervation method under physiologically friendly conditions by eliminating oil or toxic chemical, and employing low temperature and pressure for growth factor stability. Amount of 200 ng of bone morphogenetic protein-7 (BMP-7) was incorporated in the chitosan microparticles by two methods: encapsulating and coating techniques. These microparticles were tested in vivo to determine the biological response in a rat femoral bone defect at 6 and 12 weeks. Four groups (n=10) were tested which include two groups for BMP-7 incorporated microparticles (by two techniques), microparticles without BMP-7, and defect itself (negative control). Healthy bone formation was observed around the microparticles, which were only confined to the defect site and did not disperse. Histology indicated minor inflammatory response around the microparticles at 6 weeks, which reduced by 12 weeks. Micro-CT analysis of bone surface density and porosity was found to be significantly more (p<0.05) for microparticles containing groups, in comparison with controls, which suggests that the new bone formed in the presence of microparticles is more interconnected and porous. Collagen fibrils analysis conducted using multiphoton microscopy showed significant improvement in the formation of bundled collagen area (%) in microparticles containing groups in comparison with controls, indicating higher cross-linking between the fibrils. Microparticles were biocompatibe and did not degrade in the 12 week implant period.

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Section: Discussioncontrasting

confidence: 62%

Bone regeneration using injectable BMP-7 loaded chitosan microparticles in rat femoral defect

Mantripragada

Jayasuriya

2016

Materials Science and Engineering: C

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“…The 13‐93 glass composition, has been observed to show more facile viscous flow characteristics than the original 45S5 glass 20–25. Due to the wider range between the glass transition temperature and the crystallization temperature of the 13‐93 composition compared to that of the 45S5 glass, this glass can be more easily pulled into fibers and fabricated into a variety of three dimensional shapes.…”

Section: Introductionmentioning

confidence: 99%

“…19 The 13-93 glass composition, has been observed to show more facile viscous flow characteristics than the original 45S5 glass. [20][21][22][23][24][25] Due to the wider range between the glass transition temperature and the crystallization temperature of the 13-93 composition compared to that of the 45S5 glass, this glass can be more easily pulled into fibers and fabricated into a variety of three dimensional shapes. One indirect indicator of bioactivity that has been observed with 13-93 glass is the formation of a hydroxyapatite surface layer on samples immersed in simulated body fluid for 1 to 2 weeks.…”

Section: Introductionmentioning

confidence: 99%

In vitro performance of 13‐93 bioactive glass fiber and trabecular scaffolds with MLO‐A5 osteogenic cells

Modglin

Brown

et al. 2012

J Biomedical Materials Res

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This in vitro study was performed to evaluate the ability of two types of porous bioactive glass scaffolds to support the growth and differentiation of an established osteogenic cell line. The two scaffold types tested included 13-93 glass fiber and trabecular-like scaffolds seeded with murine MLO-A5 cells and cultured for intervals of 2 to 12 days. Culture in MTT-containing medium showed metabolically active cells both on the surface and within the interior of the scaffolds. Scanning electron microscopy revealed well-attached cells on both types of scaffolds with a continual increase in cell density over a 6-day period. Protein measurements also showed a linear increase in cell density during the incubation. Activity of alkaline phosphatase, a key indicator of osteoblast differentiation, increased about 10-fold during the 6-day incubation with both scaffold types. The addition of mineralization media to MLO-A5 seeded scaffolds triggered extensive formation of alizarin red-positive mineralized extracellular material, additional evidence of cell differentiation and completion of the final step of bone formation on the constructs. Collectively, the results indicate that the 13-93 glass fiber and trabecular scaffolds promote the attachment, growth, and differentiation of MLO-A5 osteogenic cells and could potentially be used for bone tissue engineering applications.

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“…Este tipo de reação é bastante comum a materiais à base de biovidro [25], bem como a praticamente qualquer biomaterial, sintético ou natural, implantado no tecido subcutâneo de roedores [26,27], ou subcutâneo de coelhos. [28] Ainda, nos períodos experimentais avaliados, não sem observou fibrose ao redor das partículas.…”

Section: Discussionunclassified

Resposta do tecido subcutâneo de camundongos à implantação de um novo biovidro à base de óxido de nióbio

Lima¹,

Silva²,

Barros³

et al. 2011

Matéria (Rio J.)

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RESUMOO objetivo deste estudo foi avaliar a resposta biológica do tecido subcutâneo de camundongos à implantação de um novo biovidro à base de óxido de nióbio e óxido fosforoso. A morfologia do material foi analisada por microscopia eletrônica de varredura com espectroscopia de raios X por dispersão de energia (MEV/EDX) e a resposta tecidual foi avaliada após implantação de 30mg do biovidro no tecido subcutâneo da região dorsal de camundongos Balb/c (n=15), segundo ISO 10993-6. Após o período de 1, 3 e 9 semanas, os animais foram sacrificados e as necrópsias fixadas em formol tamponado pH 7,2 e submetidas ao processamento clássico para inclusão em parafina. Contudo, nenhum processo convencional de desmineralização de ossos e biomateriais cerâmicos foi capaz de desmineralizar o material. Alternativamente, o material obtido foi reprocessado e incluído em resina para corte em micrótomo de impacto. O estudo histopatológico considerou para análise: reação inflamatória (intensidade de células polimorfonucleares, mononucleares e células gigantes multinucledas das do tipo corpo estranho) e processo de reparo (tecido de granulação e fibrose). A análise do biomaterial no MEV/EDS demonstrou partículas irregulares com ampla variação dimensional e presença de nióbio, fósforo, cálcio, carbono e oxigênio. A análise histológica mostrou moderado infiltrado inflamatório composto de células mononucleares na semana 1, a qual desapareceu nos períodos subsequentes. Após 3 e 9 semanas, vasos sanguíneos foram observados com presença discreta de células gigantes multinucleadas tipo corpo estranho contendo partículas de biovidro de nióbio. Mesmo após 9 semanas, não se observou presença de cápsula fibrosa ao redor do material. Em nenhum momento, verificaram-se focos de necrose nem sinais de degradação das partículas. Baseado nestes resultados preliminares foi possível concluir que o material testado é biocompatível e não-bioabsorvível. A comparação deste biovidro contendo nióbio, especialmente implantado intra-ósseo, permitirá avaliar seu real potencial uso como enxerto ósseo. Palavras chaves: biomaterial, biovidro, óxido de nióbio, biocompatibilidadeBiocompatibility in vivo study of a new bioglass made up niobium oxide ABSTRACTThe aim of this study was to evaluate the biological response to the implantation of a new biocompatible glass based on niobium oxide and phosphorus oxide. Scanning electron microscopy with energy dispersive spectroscopy evaluated the morphology of the material (SEM/EDS) and the tissue response was evaluated by implantation of 30mg of biocompatible glass on subcutaneous tissue of Balb/c mice (n=15) as ISO 10993-6. After the period of 1, 3 and 9 weeks, the animals were killed and the necropsies were fixed in buffered formalin pH 7.2 and processed for inclusion in paraffin after demineralization step in Allkymia solution; however, no

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Soft tissue reactions to bioactive glass 13‐93 combined with chitosan

Cited by 17 publications

References 35 publications

Bone regeneration using injectable BMP-7 loaded chitosan microparticles in rat femoral defect

Bone regeneration using injectable BMP-7 loaded chitosan microparticles in rat femoral defect

In vitro performance of 13‐93 bioactive glass fiber and trabecular scaffolds with MLO‐A5 osteogenic cells

Resposta do tecido subcutâneo de camundongos à implantação de um novo biovidro à base de óxido de nióbio

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