This study aims to investigate the effects of various design parameters on the actuation performance of a pneumatic network actuator (PNA), optimise its structure using the finite element method (FEM), and subsequently quantify the performance of the resulting actuator topology experimentally. The effects of the structural parameters, including the operation pressure, the wall thickness and the gap between the chambers, bottom layer thickness, and the geometry of the channel cross section, on the deformation and bending angle of the actuator were evaluated to optimise the performance of the pneumatic actuator. A Global Analysis of Variance (ANOVA) was performed to investigate how the variables affect the mechanical output of the actuator and, thus, the significance of variables affecting the deformation (and bending angle) of the pneumatic actuator was identified. After the parameter optimisation, a pneumatic channel with a 4.5 mm bottom layer thickness, 1.5 mm wall thickness, and 1.5 mm gap between sequential chambers is recommended to perform optimised bending motion for the pneumatic network actuator. The optimised FE model results were verified experimentally. This design optimisation method based on the FEM and ANOVA analysis can be extended to the topology optimisation of other soft actuators.Robotics 2018, 7, 24 2 of 16 compliance and safety. A pneumatic actuation methodology based on PneuNets' concept can generate a simple bending motion with only one pressure source [10]. The PneuNets geometry and mechanics have been investigated using a simple theoretical model [11]. This model has the potential to be used as a design principle for designing soft pneumatic autonomous actuators. The effect of material properties and chamber geometry on the performance of the actuator is described. The movements of this soft actuator are generated by pressurising the internal PneuNets, and the configuration of the actuator is determined by the structure of the channels. Air is selected as the power source for its unique features, such as compressibility, ease of storage, low viscosity, and ability to provide rapid actuation process. The same methodology has been used in a multigait quadrupedal soft robot [12]. These robots, like some animals (such as squids, starfish, and worms), have no rigid internal skeletons. The soft robot described in [13] combines microfluidics and the PneuNets concept. The pneumatic systems are used for activation and the colour-filled microfluidics channels in the thin silicone surface are used for camouflage. Various applications using these kinds of actuators have been reported [7,14,15]. A large pneumatically actuated soft robot (0.65 m in length) with an embedded controller, battery, and miniature compressors was developed in [16]. This soft robot can carry its power system and adapts to various environments. The main difference between the large robot and the PneuNets robots is that there is a small gap between the adjacent channels. It can provide a higher work output and rapid movement. Mo...