Soft nanofiber modified micropatterned substrates enhance native-like endothelium maturation via CXCR4/calcium-mediated actin cytoskeleton assembly

Yi, Baolian; Zhou, Boya; Dai, Wufei; Lu, Xinwu; Liu, Wei

doi:10.1007/s12274-022-4670-2

Cited by 3 publications

(2 citation statements)

References 71 publications

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“…These findings suggested that the fibrous structure on FG Ti may positively influence gene transfection. It has been reported that micropatterns can manipulate cell adhesion, spreading, elongation and migration by remodeling the cytoskeleton [ 20 , 43 ]. The polymerization of the F-actin network is also potentially involved in endocytosis processes, including the invagination, contraction, division and movement of vesicles [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

Fibrous topology promoted pBMP2-activated matrix on titanium implants boost osseointegration

He,

Wang,

Wang

et al. 2023

Regenerative Biomaterials

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Titanium (Ti) implants have been extensively used after surgical operations. Its surface bioactivity is of importance to facilitate integration with surrounding bone tissue, and ultimately ensure stability and long-term functionality of the implant. The plasmid DNA-activated matrix (DAM) coating on the surface could benefit osseointegration but is still trapped by poor transfection for further application, especially on the bone marrow mesenchymal stem cells (BMSCs) in vivo practical conditions. Herein, we constructed a DAM on the surface of fibrous-grained titanium (FG Ti) composed of phase-transition lysozyme (P) as adhesive, cationic arginine-rich lipid (RLS) as the transfection agent and plasmid DNA (pDNA) for bone morphology protein 2 (BMP2) expression. The cationic lipid RLS improved up to 30-fold higher transfection than that of commercial reagents (Lipofectamine 2000 and polyethyleneimine) on MSC. And importantly, Ti surface topology not only promotes the DAM to achieve high transfection efficiency (∼75.7% positive cells) on MSC due to the favorable combination but also reserves its contact induction effect for osteoblasts. Upon further exploration, the fibrous topology on FG Ti could boost pDNA uptake for gene transfection, and cell migration in MSC through cytoskeleton remodeling and induce contact guidance for enhanced osteointegration. At the same time, the cationic RLS together with adhesive P were both antibacterial, showing up to 90% inhibition rate against Escherichia coli and Staphylococcus aureus with reduced adherent microorganisms and disrupted bacteria. Finally, the FG Ti-P/pBMP2 implant achieved accelerated bone healing capacities through highly efficient gene delivery, aligned surface topological structure and increased antimicrobial properties in a rat femoral condylar defect model.

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Section: Resultsmentioning

confidence: 99%

Fibrous topology promoted pBMP2-activated matrix on titanium implants boost osseointegration

He,

Wang,

Wang

et al. 2023

Regenerative Biomaterials

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“…Liu et al exploited a micropatterned substrate decorated with soft nanofibers to strengthen cell-cell and cell-matrix interactions via electrospinning and soft lithography techniques. 152 The experimental results indicated that the well-designed substrate with parallel microgrooves and suitable modulus contributed to promoting the formation and maturation of confluent native-like endothelium via enhancing cell-cell and cell-matrix interactions. Transcriptome analysis demonstrated that the micropatterned substrate decorated with soft nanofibers was capable of accelerating endothelial remodeling via integrin α5-mediated microtubule disassembly and type I interleukin 1 receptormediated signaling pathways.…”

Section: Cardiovascular Stents For Repairing the Endothelial Barrier ...mentioning

confidence: 95%

Restoring endothelial function: shedding light on cardiovascular stent development

Wang

Feng

2023

Biomater. Sci.

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Fabrication of micro-nano patterned materials mimicking the topological structure of extracellular matrix for biomedical applications

Nie,

Han,

2023

Nano Res.

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Soft nanofiber modified micropatterned substrates enhance native-like endothelium maturation via CXCR4/calcium-mediated actin cytoskeleton assembly

Cited by 3 publications

References 71 publications

Fibrous topology promoted pBMP2-activated matrix on titanium implants boost osseointegration

Fibrous topology promoted pBMP2-activated matrix on titanium implants boost osseointegration

Restoring endothelial function: shedding light on cardiovascular stent development

Fabrication of micro-nano patterned materials mimicking the topological structure of extracellular matrix for biomedical applications

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