We study light-cone distribution amplitudes of heavy-light systems, such as a B-meson. By an explicit computation, we determine how two-parton distribution amplitudes mix with three-parton ones at one loop: φ + is shown to mix only into itself, whereas φ − mixes with the difference of three-parton distribution amplitudes Ψ A − Ψ V . We determine the corresponding anomalous dimension and we check the gauge independence of our result by considering a general covariant gauge. Finally, we comment on some implications of our result for phenomenological models of these distribution amplitudes.