Sodium selenite improves the in vitro follicular development by reducing the reactive oxygen species level and increasing the total antioxidant capacity and glutathione peroxide activity

Abedelahi, Ali; Salehnia, Mojdeh; Allameh, Abdolamir; Davoodi, Daryoush

doi:10.1093/humrep/deq002

Cited by 71 publications

(66 citation statements)

References 49 publications

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“…Preantral follicles were isolated mechanically by using a 29 gauge needle under a stereomicroscope at × 10 magnification [2]. Isolated follicles were selected according to the following criteria: rounded follicular structure with 140-160 um diameters, containing visible centrally located healthy oocyte surrounded with intact several layers of granulosa cells and at least one layer of theca cells.…”

Section: Preantral Follicle Culturementioning

confidence: 99%

“…In vitro ovulation was induced by replacement of culture media with fresh medium supplemented with 1.5 IU/ml human chronic gonadotropin (hCG) on 12th days of culture [2]. After 48 h, released oocytes were classified as germinal vesicle (GV), germinal vesicle breakdown (GVBD) when the GV was absent, and metaphase II oocytes (MII) when the first polar body was extruded.…”

Section: In Vitro Ovulation Inductionmentioning

confidence: 99%

“…MII oocytes were collected from different groups and MII oocytes (n 010) were inseminated into 50 uL droplets of T6 medium supplemented with 15 mg/ ml BSA and a final motile sperm concentration of 1-2×10 6 / ml then incubated for 4-6 h. At the end of this period, inseminated oocytes (n010) were cultured in 50 uL microdroplets of T6 medium supplemented with 5 mg/ml BSA for 120 h. The percentage of embryos developing to the blastocyte stages was determined. All fertilization steps and embryo culture were carried out under detoxified mineral oil at 37°C, 100% humidity and 5% CO2 in air [2].…”

Section: In Vitro Fertilizationmentioning

confidence: 99%

“…The production of intracellular ROS of cultured preantral follicles was measured by 2′,7′ -dichlorodihydrofluorescin (DCHF) probe [2,32]. Briefly, 10 follicles were collected from different times of culture (0, 24, 48, 72 and 97 h).…”

Section: Measurement Of Reactive Oxygen Speciesmentioning

confidence: 99%

“…Assessment of TAC in cultured pre-antral was performed according to ferric reducing/antioxidant power (FRAP) method [2,13]. For preparation of cellular supernatant, 10 follicles were collected from different times of culture period (0, 24, 48, 72 and 96 h) and homogenized in 100 ml TrisHCl buffer and sonicated at 50 W for 1 min and then centrifuged at 10000×g for 20 min at 4°C.…”

Section: Total Antioxidant Capacity Assaymentioning

confidence: 99%

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The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Talebi

Zavareh

Kashani

et al. 2012

J Assist Reprod Genet

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Purpose This study was designed to investigate the effect of alpha-lipoic acid (ALA) on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and developmental competence of cultured pre-antral follicles derived from mouse ovarian tissue. Methods Pre-antral follicles were isolated from immature mouse ovaries and were cultured in α-minimal essential medium supplemented with different concentrations (0, 50, 100, 250 and 500 uM) of ALA. Follicular growth, oocyte maturation and embryo development were evaluated. Separately, ROS and TAC were measured after 0, 24, 48, 72 and 96 h of culture with spectrofluorometery and ferric reducing/antioxidant power (FRAP) assay, respectively. Results In the presence of 100 uM ALA, developmental rates of follicles, oocytes and embryos were significantly higher than other groups (p<0.05). At 96 h after culture, a decrease in ROS and an increase in TAC were observed in ALA group compared to control group (p<0.05). Conclusion ALA (100 uM) improves the in vitro development of follicles. This effect may be mediated by decreasing ROS concentration and increasing follicular TAC level during the culture period.

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Section: Preantral Follicle Culturementioning

confidence: 99%

Section: In Vitro Ovulation Inductionmentioning

confidence: 99%

Section: In Vitro Fertilizationmentioning

confidence: 99%

Section: Measurement Of Reactive Oxygen Speciesmentioning

confidence: 99%

Section: Total Antioxidant Capacity Assaymentioning

confidence: 99%

See 3 more Smart Citations

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Talebi

Zavareh

Kashani

et al. 2012

J Assist Reprod Genet

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Bovine embryo survival under oxidative‐stress conditions is associated with activity of the NRF2‐mediated oxidative‐stress‐response pathway

Amin

Gad

Salilew‐Wondim

et al. 2014

Molecular Reproduction Devel

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In present study, we sought to examine the ability of preimplantation bovine embryos to activate the NF-E2-related factor 2 (NRF2)-mediated oxidative-stress response under an oxidative stress environment. In vitro 2-, 4-, 8-, 16-cell-, and blastocyst-stage embryos were cultured under low (5%) or high (20%) oxygen levels. The expression of NRF2, KEAP1 (NRF2 inhibitor), antioxidants downstream of NRF2, and genes associated with embryo metabolism were analyzed between the embryo groups using real-time quantitative PCR. NRF2 and KEAP1 protein abundance, mitochondrial activity, and accumulation of reactive oxygen species (ROS) were also investigated in blastocysts of varying competence that were derived from high- or low-oxygen levels. The expression levels of NRF2 and its downstream antioxidant genes were higher in 8-cell, 16-cell, and blastocyst stages under high oxygen tension, whereas KEAP1 expression was down-regulated under the same conditions. Higher expression of NRF2 and lower ROS levels were detected in early (competent) blastocysts compared to their late (noncompetent) counterparts in both oxygen-tension groups. Similarly, higher levels of active nuclear NRF2 protein were detected in competent blastocysts compared to their noncompetent counterparts. Thus, the survival and developmental competence of embryos cultured under oxidative stress are associated with activity of the NRF2-mediated oxidative stress response pathway during bovine pre-implantation embryo development.

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Role of Reactive Oxygen Species in the Correlation Between Host and Microbes

Kim

Lee

2019

Oxidative Stress in Microbial Diseases

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Sodium selenite improves the in vitro follicular development by reducing the reactive oxygen species level and increasing the total antioxidant capacity and glutathione peroxide activity

Abstract: SS caused an increase in follicular TAC level and GPx activity and a decrease in ROS level, thus improving the in vitro development of follicles.

Cited by 71 publications

References 49 publications

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Bovine embryo survival under oxidative‐stress conditions is associated with activity of the NRF2‐mediated oxidative‐stress‐response pathway

Role of Reactive Oxygen Species in the Correlation Between Host and Microbes

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