Sodium pertechnetate (Na99mTcO4) biodistribution in mice exposed to cigarette smoke

Lima, Elaine A. C.; ., G.F. Diré; Bernardo-Filho, Mário; Pôrto, Luís Cristóvão

doi:10.1186/1471-2385-5-1

Cited by 20 publications

(16 citation statements)

References 28 publications

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“…Equation (3) [10, 11] was used to calculate drug concentration in each organ, in which ID meant injected dose and pure M was pure weight of each organ(s) as follows:

\begin{matrix} % \frac{ID}{gr  Organ} = \frac{eac h_{count}}{tota l_{count}} \times \frac{100}{pure M} . \end{matrix}

…”

Section: Methodsmentioning

confidence: 99%

Biodistribution of Amikacin Solid Lipid Nanoparticles after Pulmonary Delivery

Varshosaz

Ghaffari²,

Mirshojaei

et al. 2013

BioMed Research International

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The main purpose of the present work was studying the biodistribution of amikacin solid lipid nanoparticles (SLNs) after pulmonary delivery to increase its concentration in the lungs for treatment of cystic fibrosis lung infections and also providing a new method for clinical application of amikacin. To achieve this aim, 99mTc labelled amikacin was loaded in cholesterol SLNs and after in vitro optimization, the desired SLNs and free drug were administered through pulmonary and i.v. routes to male rats and qualitative and biodistribution studies were done. Results showed that pulmonary delivery of SLNs of amikacin by microsprayer caused higher drug concentration in lungs than kidneys while i.v. administration of free drug caused reverse conditions. It seems that pulmonary delivery of SLNs may improve patients' compliance due to reduction of drug side effects in kidneys and elongation of drug dosing intervals due to the sustained drug release from SLNs.

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“…Equation (3) [10, 11] was used to calculate drug concentration in each organ, in which ID meant injected dose and pure M was pure weight of each organ(s) as follows:

\begin{matrix} % \frac{ID}{gr  Organ} = \frac{eac h_{count}}{tota l_{count}} \times \frac{100}{pure M} . \end{matrix}

…”

Section: Methodsmentioning

confidence: 99%

Biodistribution of Amikacin Solid Lipid Nanoparticles after Pulmonary Delivery

Varshosaz

Ghaffari²,

Mirshojaei

et al. 2013

BioMed Research International

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“…Some authors have described that natural and synthetic products can alter the labelling of blood constituents with 99m Tc (Frydman et al, 2004;Valenca et al, 2005;Fonseca et al, 2005). The labelling of blood constituents could decrease due to the action of drugs in (1) binding at the same sites on the blood constituents, (2) direct inhibition (chelating action) of the stannous and pertechnetate ions, (3) direct oxidation or generation of free radicals that could oxidize the stannous ion, (4) antioxidant action impeding or decreasing the stannous ion oxidization, and (5) alteration of the plasma membrane structure or modifying the transport systems of stannous and pertechnetate ions into cells.…”

Section: Resultsmentioning

confidence: 99%

Guava extract (Psidium guajava) alters the labelling of blood constituents with technetium-99m

Abreu

Bernardo

et al. 2006

J. Zhejiang Univ. - Sci. B

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Psidium guajava (guava) leaf is a phytotherapic used in folk medicine to treat gastrointestinal and respiratory disturbances and is used as anti-inflammatory medicine. In nuclear medicine, blood constituents (BC) are labelled with technetium-99m ( 99m Tc) and used to image procedures. However, data have demonstrated that synthetic or natural drugs could modify the labelling of BC with 99m Tc. The aim of this work was to evaluate the effects of aqueous extract of guava leaves on the labelling of BC with 99m Tc. Blood samples of Wistar rats were incubated with different concentrations of guava extract and labelled with 99m Tc after the percentage of incorporated radioactivity (%ATI) in BC was determined. The results suggest that aqueous guava extract could present antioxidant action and/or alters the membrane structures involved in ion transport into cells, thus decreasing the radiolabelling of BC with 99m Tc. The data showed significant (P<0.05) alteration of ATI in BC from blood incubated with guava extract.

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“…It is described that natural and synthetic products can alter the labeling of blood constituents with 99mTc (Frydman et al, 2004;Valenca et al, 2005;Fonseca et al, 2005). The labeling of blood constituents could decrease due to the action of drugs (natural and synthetic) in (1) binding at the same sites on the blood constituents, (2) direct inhibition (chelating action) of the stannous (Sn +2 ) and pertechnetate ions (99mTcO -), (3) direct oxidation or generation of free radicals that could oxidize the stannous ion, (4) antioxidant action impeding or decreasing the stannous ion oxidation, and (5) alteration of the plasma membrane structure or modifying the transport systems of stannous and pertechnetate ions into cells.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the effect of Bacopa monnieri (L.) Wettst. extract on the labeling of blood elements with technetium-99m and on the morphology of red blood cells

Kyle¹,

Chandra²,

Misra³

2009

Rev. bras. farmacogn.

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RESUMO: "Avaliação do efeito do extrato de Bacopa monnieri (L.) Wettst. na marcação de elementos sanguíneos com tecnécio-99m e na morfologia de células vermelhas do sangue." Bacopa monnieri (L.) Wettst. (BM), uma planta tradicional da medicina ayurvédica, é usada por séculos para problemas de memória, antiinflamatória, antitérmica, sedativa e como agente anti-epiléptico. O extrato BM têm sido extensivamente investigada por diversos autores por seus efeitos neurofarmacológicas. Na medicina nuclear, os glóbulos vermelhos (RBC) marcados com tecnécio-99m (99mTc) tem várias aplicações clínicas. Entretanto, os dados demonstraram que drogas sintéticas ou naturais podem modificar a marcação de hemácias com 99mTc. Como Bacopa monnieri é amplamente utilizado em medicina, foi avaliada a sua influência na marcação de hemácias e proteínas plasmáticas com tecnécio-99m (99mTc). Este procedimento de marcação depende de um agente redutor e normalmente o cloreto estanoso é usado. Sangue foi incubado com os extratos BM. Solução de cloreto estanoso e 99mTc foram adicionados. O sangue foi centrifugado e o plasma (P) e células sangüíneas (CS) foram isolados. Amostras de P ou BC também foram precipitadas, centrifugadas e fração insolúvel (FI) e fração solúvel (FS) foram separadas. A porcentagem de radioatividade (%ATI) em BC, IF-BC e SE-P foram calculados. A As% ATI diminuiu significativamente em BC de 95,53±0,45-35,41±0,44, no IF-P de 80,20±1,16 para 7,40±0,69 e no IF-BC em 73,31±1,76-21,26±1,40. O estudo da morfologia de hemácias revelou alterações morfológicas importantes devido a tratamentos com extratos BM. Sugere-se que a ação do extrato BM poderia ser explicada por uma inibição dos íons estanoso e pertecnetato ou oxidação do íon estanoso ou por danos na membrana plasmática.Unitermos: Bacopa monnieri, Scrophulariaceae, tecnécio-99m, células sanguíneas vermelhas, plasma, proteinas, morphologia.ABSTRACT: Bacopa monnieri (L.) Wettst. (BM), a traditional Ayurvedic medicine, used for centuries as a memory enhancing, anti-inflammatory, antipyretic, sedative and antiepileptic agent. BM extract have been extensively investigated by several authors for their neuropharmacological effects. In nuclear medicine, red blood cells (RBC) labeled with technetium-99m (99mTc) have several clinical applications. However, data have demonstrated that synthetic or natural drugs could modify the labeling of RBC with 99mTc. As Bacopa monnieri is extensively used in medicine, we evaluated its influence on the labeling of RBC and plasma proteins using technetium-99m (99mTc). This labeling procedure depends on a reducing agent and usually stannous chloride is used. Blood was incubated with BM extracts. Stannous chloride solution and 99mTc were added. Blood was centrifuged and plasma (P) and blood cells (BC) were isolated. Samples of P or BC were also precipitated, centrifuged and insoluble fraction (IF) and soluble fraction (SF) were separated. The percentage of radioactivity (%ATI) in BC, IF-BC and IF-P were calculated. The %ATI significantly decreased on ...

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Sodium pertechnetate (Na99mTcO4) biodistribution in mice exposed to cigarette smoke

Abstract: Background: The biological effects of cigarette smoke are not fully known. To improve our understanding of the action of various chemical agents, we investigated the biodistribution of sodium pertechnetate (Na 99m TcO 4 ) in mice exposed to cigarette smoke.

Cited by 20 publications

References 28 publications

Biodistribution of Amikacin Solid Lipid Nanoparticles after Pulmonary Delivery

Biodistribution of Amikacin Solid Lipid Nanoparticles after Pulmonary Delivery

Guava extract (Psidium guajava) alters the labelling of blood constituents with technetium-99m

Assessment of the effect of Bacopa monnieri (L.) Wettst. extract on the labeling of blood elements with technetium-99m and on the morphology of red blood cells

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