Sodium fluoride and chromosome damage (<i>in vitro</i> human lymphocyte and <i>in vivo</i> micronucleus assays)

Albanese, R.

doi:10.1093/mutage/2.6.497

Cited by 21 publications

(6 citation statements)

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“…No published studies were found which have directly assessed the genotoxic potential of sodium fluoride to osteoblasts, but sodium fluoride has been found positive in assays for gene-mutation induction in mammalian cells and in Drosophila (Crespi et al, 1990;Cole et al, 1986;Caspary et al, 1987;Mitchell and Gerdes, 1973). It is positive in some plant and animal systems for the induction of chromosomal aberrations (Aardema et al, 1989;Albanese, 1987;Scott and Roberts, 1987;Gala1 and Abd-Alla, 1976), and it is positive in in vitro assays for morphologic transformation of Syrian hamster ovary cells (Tsutsui et al, 1984;Jones et al, 1988;Lasne ef al., 1988). Other investigators have failed to find evidence of genotoxic effects of fluorides in some of these same test systems, and the significance of many of the positive findings remains a point of contention.…”

Section: Resultsmentioning

confidence: 42%

“…It is positive in some plant and animal systems for the induction of chromosomal aberrations (Aardema et al, 1989;Albanese, 1987;Scott and Roberts, 1987;Gala1 and Abd-Alla, 1976), and it is positive in in vitro assays for morphologic transformation of Syrian hamster ovary cells (Tsutsui et al, 1984;Jones et al, 1988;Lasne ef al., 1988). Other investigators have failed to find evidence of genotoxic effects of fluorides in some of these same test systems, and the significance of many of the positive findings remains a point of contention.…”

Section: Resultsmentioning

confidence: 99%

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Results and conclusions of the national toxicology program's rodent carcinogenicity studies with sodium fluoride

Bucher

Hejtmancik

Toft

et al. 1991

Intl Journal of Cancer

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The US National Toxicology Program (NTP) has conducted toxicity and carcinogenicity studies with sodium fluoride administered in the drinking water to F344/N rats and B6C3F1 mice. The drinking water concentrations used in the 2-year studies were 0, 25, 100, or 175 ppm sodium fluoride (equivalent to 0, 11, 45 or 79 ppm fluoride). Survival and weight gains of rats and mice were not affected by fluoride treatment. Animals receiving sodium fluoride developed effects typical of dental fluorosis, and female rats given 175 ppm had increased osteosclerosis. There were no increases in neoplasms in female rats or in male or female mice that were attributed to sodium fluoride administration. There was equivocal evidence of carcinogenic activity of sodium fluoride in male rats based on the occurrence of a small number of osteosarcomas in treated animals.

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Section: Resultsmentioning

confidence: 42%

Section: Resultsmentioning

confidence: 99%

Results and conclusions of the national toxicology program's rodent carcinogenicity studies with sodium fluoride

Bucher

Hejtmancik

Toft

et al. 1991

Intl Journal of Cancer

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“…Similarly, osteoblasts from mice incubated with NaF (0,0.5,1,2,4,8,12,or 20 mg F − /L) in vitro for 24, 48, or 72 h showed significantly elevated cell viability by MTT assay after incubation with low concentrations of F (0.5 to approximately 2 mg F − /L) for 24 to approximately 72 h (Xu et al, 2008). Previous in vitro investigations demonstrated significant genotoxic effects attributed to F: clastogenicity, induction of chromosomal aberrations (CA), sister chromatid exchanges (SCE), and MN formation (Albanese 1987;Chen et al, 2000;Khalil 1995;Kleinsasser et al, 2001;Kishi and Tonomura 1984;Mihashi and Tsutsui 1996;Suzuki et al, 1991). Similarly, our study revealed NaF induced double-strand breaks in DNA as represented by comet assay and increased frequency in MN formation, although this was not statistically significant.…”

Section: Discussionmentioning

confidence: 96%

Sodium Fluoride Promotes Apoptosis by Generation of Reactive Oxygen Species in Human Lymphocytes

Jothiramajayam¹,

Sinha²,

Ghosh³

et al. 2014

Journal of Toxicology and Environmental Health, Part A

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Fluoride generated the attention of toxicologists due to its deleterious effects at high concentrations in human populations suffering from fluorosis and with in vivo experimental models. Interest in its undesirable effects has resurfaced due to the awareness that this element interacts with cellular systems even at low doses. This study focused on examining the adverse effects of inorganic fluoride (NaF) on human lymphocyte cells in vitro. Mitochondrial function, oxidative stress, cell cycle progression, and mode of cell death were combined with genotoxic endpoints. Data demonstrated that NaF at lower concentrations, although not significantly cytotoxic and genotoxic, induced oxidative stress leading to apoptotic cell death. The results also suggested that at low concentrations (<1 μg/ml), NaF may affect cell cycle progression. Taken together, our findings confirm earlier reports on mechanisms involved in NaF-induced apoptosis.

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“…Chromosome aberrations were induced in cultured human lymphocytes and fibroblasts at doses in the range of 20-40 pg NaF/ml [Albanese, 1987;Scott and Roberts, 1987;Tsutsui et al, 1984~). and in leukocytes at doses in the range of 0.03-3.15 mM F-(1-132 pg NaF/ml) [Jachimczak and Skotarczak, 19781.…”

Section: Human Cellsmentioning

confidence: 99%

“…1989NTP, 1990Tsutsui et al, 1984bIshidate, 1987NTP, 1990. Li et al, 1987dTong et al, 1988Tsutsui et al, 1984bIshidate, 1987He et al, 1983He et al, 1983Albanese. 1987Sat0 et al, 1989Jachimczak and Scott and Roberts, 1987Suzuki and Tsutsui.…”

Section: Human Cellsmentioning

confidence: 99%

Genetic toxicity of fluoride

Zeiger

Shelby

Witt

1993

Environ and Mol Mutagen

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F- is not mutagenic in standard bacterial systems, but produces chromosome aberrations and gene mutations in cultured mammalian cells. Although there is disagreement in the literature concerning the ability of F- to induce chromosome aberrations in cultured human and rodent cells, the weight of the evidence leads to the conclusion that F- exposure results in increased chromosome aberrations in these test systems. NaF induced primarily chromatid gaps and chromatid breaks, indicating that the rodent cells are responsive in the G2 stage of the cell cycle. In contrast, studies with synchronized human cells indicated that the S phase was the most sensitive. If F- does have a cell cycle-specific effect, it could be expected that differences in the cell treatment and harvest protocols could lead to conflicting results for the induction of chromosome aberrations. Gene mutations were produced in cultured rodent and human cells in the majority of the studies. Unfortunately, a number of the in vitro and in vivo cytogenetic studies are of questionable utility because of the protocols used, the quality of the responses reported, or the interpretations of the data. The conflicting results in the in vivo cytogenetic studies are difficult to reconcile. There are reports of increased chromosome aberrations in rat bone marrow and testes, but other studies, using similar protocols and dose ranges, have reported no induced chromosome damage. Although some of the studies were performed at toxic levels of F-, other studies, including those that showed positive results, were at F- concentrations (1-5 ppm) equivalent to human exposure levels. In the majority of studies that were reported to be positive, there were high background frequencies, or the investigators reported categories of nuclear or chromosome damage that are difficult to interpret. Interestingly, many of the positive results were obtained when anaphase cells were scored, whereas similar treatment protocols in other laboratories yielded negative results when metaphase cells were the only cell type examined. It is difficult, without additional data, to determine the reasons for finding chromosome breaks in anaphase, but not metaphase, cells. Other reports have presented insufficient information to allow adequate evaluations. Therefore, at this time, the question of whether F- produces chromosome damage in vivo should be considered unresolved.

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Sodium fluoride and chromosome damage (in vitro human lymphocyte and in vivo micronucleus assays)

Cited by 21 publications

References 0 publications

Results and conclusions of the national toxicology program's rodent carcinogenicity studies with sodium fluoride

Results and conclusions of the national toxicology program's rodent carcinogenicity studies with sodium fluoride

Sodium Fluoride Promotes Apoptosis by Generation of Reactive Oxygen Species in Human Lymphocytes

Genetic toxicity of fluoride

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