Sodium-dependent pH regulation in active sea urchin sperm

Bibring, Thomas; Baxandall, Jane; Harter, Christine C.

doi:10.1016/0012-1606(84)90157-x

Cited by 45 publications

(15 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These observations suggest that changes in plasma membrane potential and/or in internal pH could be involved in the initiation of sperm motility, a result already demonstrated in invertebrates (Schackmann et al, 1981(Schackmann et al, , 1984Christen et al, 1982Christen et al, , 1983Christen et al, , 1986Lee, 1984;Bibring et al, 1984; for review Busa and Nuccitelli, 1984;Trimmer and Vacquier, 1986) and vertebrates (Wong et al, 1981;Wong and Lee, 1983;Babcock et al, 1983; see also Busa and Nuccitelli, 1984).…”

mentioning

confidence: 71%

Ionic regulation of the plasma membrane potential of rainbow trout (Salmo gairdneri) spermatozoa: Role in the initiation of sperm motility

Gatti¹,

Billard²,

Christen³

1990

Journal Cellular Physiology

View full text Add to dashboard Cite

The ionic dependence of the trout sperm plasma membrane potential was analysed by measuring the accumulation of the lipophilic ions 3H-tetraphenylphosphonium (TPP) and 14C-thiocyanate (SCN) following dilution in artificial media isotonic to the seminal fluid. Our data showed that the trout sperm plasma membrane has a mixed conductance: the plasma membrane potential is sensitive upon the transmembrane gradients of K+, Na+, and H+. This potential is negative (less than -40 mV) in a 125 mM choline chloride media (ChM) at pH 8.5. Replacement of choline by sodium has a small depolarizing effect. The membrane potential is about -15 mV in a 125 mM potassium chloride and falls near zero mV only if valinomycin is added. In ChM changing the external pH (pHe) greatly affects the membrane potential: its value rises from less than -40 mV at pHe 9.0 to -17 mV at pHe 5.0. This pH effect is observed also in presence of sodium or potassium. A decrease in the transmembrane proton gradient produced by increasing internal pH without changing pHe induces also a depolarisation of the plasma membrane. In the different media in which trout sperm remain immotile after dilution (media with [K+] greater than 20-40 mM or a pH less than 7.5) the plasma membrane is more depolarized than in media allowing motility, suggesting a relationship between the state of membrane polarization and the intracellular effectors of the axonemal movement.

show abstract

mentioning

confidence: 71%

Ionic regulation of the plasma membrane potential of rainbow trout (Salmo gairdneri) spermatozoa: Role in the initiation of sperm motility

Gatti¹,

Billard²,

Christen³

1990

Journal Cellular Physiology

View full text Add to dashboard Cite

show abstract

“…Contact with seawater causes an influx of external Na + into the sperm that triggers the release of excess H + ions from within the cell, elevating pH i . The rise in pH i triggers the activity of dynein ATPase, which in turn initiates respiration in the sperm (Bibring et al, 1984;Christen et al, 1983;Lee et al, 1983). After activation, sea urchin sperm are reliant on endogenous sources for their energy metabolism (Mita and Nakamura, 1998), and activity levels decrease over time as energy resources become depleted (Levitan, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Once sperm are released into seawater, an uptake of sodium (Na + ) into the sperm cell triggers a release of hydrogen (H + ) ions (Bibring et al, 1984;Lee et al, 1983), causing a corresponding increase in internal pH (pH i ). This increased pH i activates dynein-ATPase, which initiates mitochondrial activity and subsequent sperm motility (Christen et al, 1982(Christen et al, , 1983Hamamah and Gatti, 1998).…”

Section: Introductionmentioning

confidence: 99%

Ocean acidification impacts on sperm mitochondrial membrane potential bring sperm swimming behaviour near its tipping point

Binet

Havenhand

Doyle³

et al. 2015

Journal of Experimental Biology

View full text Add to dashboard Cite

Broadcast spawning marine invertebrates are susceptible to environmental stressors such as climate change, as their reproduction depends on the successful meeting and fertilization of gametes in the water column. Under near-future scenarios of ocean acidification, the swimming behaviour of marine invertebrate sperm is altered. We tested whether this was due to changes in sperm mitochondrial activity by investigating the effects of ocean acidification on sperm metabolism and swimming behaviour in the sea urchin Centrostephanus rodgersii. We used a fluorescent molecular probe (JC-1) and flow cytometry to visualize mitochondrial activity (measured as change in mitochondrial membrane potential, MMP). Sperm MMP was significantly reduced in ΔpH −0.3 (35% reduction) and ΔpH −0.5 (48% reduction) treatments, whereas sperm swimming behaviour was less sensitive with only slight changes (up to 11% decrease) observed overall. There was significant inter-individual variability in responses of sperm swimming behaviour and MMP to acidified seawater. We suggest it is likely that sperm exposed to these changes in pH are close to their tipping point in terms of physiological tolerance to acidity. Importantly, substantial inter-individual variation in responses of sperm swimming to ocean acidification may increase the scope for selection of resilient phenotypes, which, if heritable, could provide a basis for adaptation to future ocean acidification.

show abstract

“…From the ionic depednency of the pHi-increase, we suggest that the Na+/H+ exchange system is important in starfish sperm as it is reported to be in sea urchin sperm (15,(17)(18)(19) and eggs (20). Studies on the effects of specific inhibitors of the exchange systems, such as amiloride, should be interesting.…”

Section: Discussionmentioning

confidence: 99%

Intracellular pH Changes of Starfish Sperm Upon the Acrosome Reaction

et al. 1986

View full text Add to dashboard Cite

The acrosome reaction is accompanied by ionic changes such as increases in intracellular Ca2+ and intracellular pH (pHi). Since the two jelly components essential for inducing the acrosome reaction, ARIS and Co-ARTS, were shown to activate Ca-channels (accompanying paper), we examined the jelly components to determine which was responsible for the pHi-increase using 9-aminoacridine as a probe of pHi. This paper presents evidence that an oligopeptide(s) is responsible for the pH,-increase. The pH, of swimming sperm is 7.4-7.5. Within 20 sec after the addition of jelly, their pH, increased rapidly by 0.06 pH unit, then decreased by 0.2-0.3 pH unit, and reached a plateau level within 3 min. Similar changes in pH, were observed on addition of a Pronase digest of ARIS (P-ARTS) and a diffusible fraction of jelly (Fraction M,) together. Fraction M,, but not ARIS or Co-ARIS increased the pHi, and activated sperm respiration in sea water at pH 6.5. The two activities of Fraction M, depended upon Naf but not Ca2+, and were susceptible to Pronase digestion. Fraction M, is also known to enhance induction of the acrosome reaction by the Ca-ionophore A23187. These results suggest that the egg jelly contains a peptide($ that is not obligatory for the acrosome reaction but facilitates the reaction by increasing the pH, of the sperm. The significance of the pHi-increase upon the acrosome reaction is discussed.

show abstract

Sodium-dependent pH regulation in active sea urchin sperm

Cited by 45 publications

References 31 publications

Ionic regulation of the plasma membrane potential of rainbow trout (Salmo gairdneri) spermatozoa: Role in the initiation of sperm motility

Ionic regulation of the plasma membrane potential of rainbow trout (Salmo gairdneri) spermatozoa: Role in the initiation of sperm motility

Ocean acidification impacts on sperm mitochondrial membrane potential bring sperm swimming behaviour near its tipping point

Intracellular pH Changes of Starfish Sperm Upon the Acrosome Reaction

Contact Info

Product

Resources

About