Sodium chloride stimulates the biomass and astaxanthin production by Haematococcus pluvialis via a two-stage cultivation strategy

Li, Qingqing; You, Jinkun; Qiao, Tengsheng; Zhong, Du-bo; Yu, Xuya

doi:10.1016/j.biortech.2021.126214

Cited by 28 publications

(19 citation statements)

References 43 publications

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“…pluvialis from the macrozooid (green) stage to the hematocyst (red) stage, which leads to astaxanthin accumulation. − Thus, H. pluvialis cultures have been subjected to various stresses, such as abiotic and chemical treatments, in efforts to increase astaxanthin biosynthesis up to commercially viable levels. − For example, it was found that electrical stimulation improved the growth of H. pluvialis cultures and consequently their accumulation of astaxanthin .…”

Section: Introductionmentioning

confidence: 99%

Supplementation with rac-GR24 Facilitates the Accumulation of Biomass and Astaxanthin in Two Successive Stages of Haematococcus pluvialis Cultivation

Wang

Mou

Qin

et al. 2022

J. Agric. Food Chem.

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The unicellular freshwater green alga Haematococcus pluvialis has attracted much research attention due to its biosynthetic ability for large amounts of astaxanthin, a blood-red ketocarotenoid that is used in cosmetics, nutraceuticals, and pharmaceuticals. Recently, numerous studies have investigated the functions of natural astaxanthin; however, the high cost of the production of astaxanthin from H. pluvialis cultures restricts its commercial viability. There is an urgent need to fulfill commercial demands by increasing astaxanthin accumulation from H. pluvialis cultures. In this study, we discovered that treatment of H. pluvialis cultures at the beginning of the macrozooid stage (day 0) with 1 μM rac-GR24, a synthetic analogue of strigolactones (a class of phytohormones), led to significant increases in biomass [up to a maximum dry cell weight (DCW) of 0.53 g/L] during the macrozooid stage and astaxanthin (from 0.63 to 5.32% of DCW) during the hematocyst stage. We elucidated that this enhancement of biomass accumulation during the macrozooid stage by rac-GR24 is due to its increasing CO 2 utilization efficiency in photosynthesis and carbohydrate biosynthesis. We also found that rac-GR24 stimulated the overproduction of nicotinamide adenine dinucleotide phosphate (NADPH) and antioxidant enzymes in H. pluvialis cultures, which alleviated the oxidative damage caused by reactive oxygen species generated during the hematocyst stage due to the exhaustion of nitrogen supplies. Moreover, rac-GR24 treatment of H. pluvialis synergistically altered the activity of the pathways of fatty acid biosynthesis and astaxanthin esterification, which resulted in larger amounts of astaxanthin being generated by rac-GR24-treated cultures than by controls. In summary, we have developed a feasible and economic rac-GR24-assisted strategy that increases the amounts of biomass and astaxanthin generated by H. pluvialis cultures, and have provided novel insights into the mechanistic roles of rac-GR24 to achieve these effects.

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Section: Introductionmentioning

confidence: 99%

Supplementation with rac-GR24 Facilitates the Accumulation of Biomass and Astaxanthin in Two Successive Stages of Haematococcus pluvialis Cultivation

Wang

Mou

Qin

et al. 2022

J. Agric. Food Chem.

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“…Low salt concentration can promote the growth of some microalgae, such as cultures of Scenedesmus sp. [80], Botryococcus braunii [81] and H. pluvialis [79]. Treatment of H. pluvialis with low-dose NaCl (12.5 mg/L) showed a promoting effect on biomass concentration (28% higher) and productivity (from 0.15 d −1 -0.22 d −1 ).…”

Section: Downstream Signalingmentioning

confidence: 98%

“…Salinity-induced signals may then have effects on the gene expression of microalgae cells, which is related to salt concentrations [79]. Low salt concentration can promote the growth of some microalgae, such as cultures of Scenedesmus sp.…”

Section: Downstream Signalingmentioning

confidence: 99%

Carotenoid Production from Microalgae: Biosynthesis, Salinity Responses and Novel Biotechnologies

et al. 2021

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Microalgae are excellent biological factories for high-value products and contain biofunctional carotenoids. Carotenoids are a group of natural pigments with high value in social production and human health. They have been widely used in food additives, pharmaceutics and cosmetics. Astaxanthin, β-carotene and lutein are currently the three carotenoids with the largest market share. Meanwhile, other less studied pigments, such as fucoxanthin and zeaxanthin, also exist in microalgae and have great biofunctional potentials. Since carotenoid accumulation is related to environments and cultivation of microalgae in seawater is a difficult biotechnological problem, the contributions of salt stress on carotenoid accumulation in microalgae need to be revealed for large-scale production. This review comprehensively summarizes the carotenoid biosynthesis and salinity responses of microalgae. Applications of salt stress to induce carotenoid accumulation, potentials of the Internet of Things in microalgae cultivation and future aspects for seawater cultivation are also discussed. As the global market share of carotenoids is still ascending, large-scale, economical and intelligent biotechnologies for carotenoid production play vital roles in the future microalgal economy.

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“…Astaxanthin (3,3′-dihydroxy-β,β-carotene-4,4′-dione) is a red ketocarotenoid with high antioxidant activity and has been widely used as multifarious pharmaceutical agents, feed additives, and functional foods . Microalgae represent the leading producers of natural astaxanthin. , …”

Section: Introductionmentioning

confidence: 99%

“…The high-value carotenoid astaxanthin also tends to be biosynthesized for accumulation in certain microalgae under such TAG-producing stress conditions, , pointing to the feasibility of utilizing these microalgae for the integrated production of the two compounds. Haematococcus pluvialis and Chromochloris zofingiensis represent the most studied microalgae for astaxanthin biosynthesis and production. , Although C. zofingiensis has a relatively low astaxanthin content as compared to H.…”

Section: Introductionmentioning

confidence: 99%

Rutin Stimulates the Green Alga Chromochloris zofingiensis for Improved Biomass and Astaxanthin Production

Ding

Liu

2022

J. Agric. Food Chem.

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Chromochloris zofingiensis represents a potential algal producer of the value-added ketocarotenoid astaxanthin. Here, rutin, a low-cost flavonoid compound, was evaluated regarding its roles in C. zofingiensis production under astaxanthin-inducing conditions via physiological, biochemical, and transcriptomics analyses. The rutin treatment allowed C. zofingiensis to achieve 81.2% more biomass and 20.5% greater astaxanthin content under nitrogen deprivation, leading to more than doubled astaxanthin production. The rutin-treated C. zofingiensis had higher levels of chlorophylls, proteins, and lipids and lower carbohydrate level than the control. Rutin promoted the intracellular abscisic acid (ABA) level, which could be restored by the ABA biosynthesis inhibitor, accompanied by the restoration of biomass concentration and astaxanthin content. The application of exogenous ABA to C. zofingiensis also furthered biomass concentration and astaxanthin accumulation. Together with the comparative transcriptomics analysis, our study provides implications into the involvement of ABA in rutin-mediated stimulation of C. zofingiensis growth and astaxanthin accumulation and highlights a feasible strategy of combining stress and chemical induction for improved microalgal production.

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Sodium chloride stimulates the biomass and astaxanthin production by Haematococcus pluvialis via a two-stage cultivation strategy

Cited by 28 publications

References 43 publications

Supplementation with rac-GR24 Facilitates the Accumulation of Biomass and Astaxanthin in Two Successive Stages of Haematococcus pluvialis Cultivation

Supplementation with rac-GR24 Facilitates the Accumulation of Biomass and Astaxanthin in Two Successive Stages of Haematococcus pluvialis Cultivation

Carotenoid Production from Microalgae: Biosynthesis, Salinity Responses and Novel Biotechnologies

Rutin Stimulates the Green Alga Chromochloris zofingiensis for Improved Biomass and Astaxanthin Production

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