Sodium Chloride Modified Silica Nanoparticles as a Non-Viral Vector with a High Efficiency of DNA Transfer into Cells

Chen, Yuxiang; Xue, Zhigang; Zheng, Duo; Xia, Kun; Zhao, Yanzhong; Liu, Ting; Long, Zhigao; Xia, Jiahui

doi:10.2174/1566523034578339

Cited by 71 publications

(50 citation statements)

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“…Coincubation of GC with gold nanoparticles (1) allowed nanoparticles to travel across GC membranes into organelles and potentially alter organelle ultrastructure, and (2) altered estradiol secretion/accumulation in a time-dependent manner. These are important observations since few studies have been published on translocation of nanoparticles into cells [18,[22][23][24][25][26][27], particularly cells of the female reproductive system. Researchers have reported movement of different types of nanoparticles to other areas of the body, but most of these studies described whole-animal studies with injection of nanoparticles or inhalation of these particles [16,18,23,28].…”

Section: Discussionmentioning

confidence: 99%

Gold Nanoparticles Enter Rat Ovarian Granulosa Cells and Subcellular Organelles, and Alter <i>In-Vitro</i> Estrogen Accumulation

Stelzer

Hutz

2009

Journal of Reproduction and Development

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Abstract. Nanoparticle technology refers to research and technology developed at the atomic or molecular level for materials of approximately 1-100 nm in length. Through accidental or involuntary exposure, nanoparticles are potentially toxic to the body, including reproductive organs. Ovarian granulosa cells play a major role in maintaining ovarian function, health, and female fertility. Since these cells are involved in steroidogenesis, we wished to evaluate whether nanoparticles affected them after traversing their membranes. Cells were co-incubated with 10 nm gold particles for up to 24 h. Transmission electron micrographs were taken of GC treated with 10 nm gold particles in order to compare and contrast ultrastructural locations of nanoparticles with treatment. From micrograph comparisons of treated vs. untreated GC at various culture times, it appeared that some intracellular organelles involved in steroidogenesis were infiltrated and/or altered due to the presence of the nanogold particles. Medium samples were taken in order to determine estradiol-17beta (E2) accumulation/secretion by untreated vs. treated cells. GC incubated with 10 nm nanogold particles for 1, 3, or 5 h were found to accumulate significantly increased amounts of estrogen compared with untreated cells. Conversely, at 24 h there was a significant attenuation with respect to controls. The data presented here provide insight into the toxicologic effects gold nanoparticles elicit on ovarian granulosa cells. Key words: Estrogen, Granulosa cells, Nanoparticles, Steroidogenesis, Transmission electron microscopy (J. Reprod. Dev. 55: [685][686][687][688][689][690] 2009) anomaterial technology has become a dynamic and exciting topic in biology over the past ten years. A decade ago nanoparticles were studied because of their size-dependent physical and chemical properties. Now they have entered a commercial exploration period [1]. Nanomaterials come in a variety of shapes, sizes, and elemental forms and are currently produced in a wide variety of types for a plethora of applications; fullerenes, carbon nanotubes, metal and metal oxide particles, polymer nanoparticles, and quantum dots are among the most common [2][3][4][5]. At these "nano" sizes, the toxicologic effects of common elements such as carbon, silicon, gold and titanium have not been studied very closely.In order for nanoparticles to exert toxicologic effects they must first cross the cell membrane. It is important to study the process by which nanoparticles gain access to the cell because of their possible toxicologic and health implications [6][7][8][9][10][11][12][13][14][15][16][17][18]; however, to date there have been few studies on this area of concern. The increased use of nanoparticles in research, medicine, and the manufacture of consumer products, therefore, creates an urgent need to test the toxic potential of these materials. Considering that nanoparticles can travel to distant locations in the body and move across cell membranes of many types of cells, it is certainly co...

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Section: Discussionmentioning

confidence: 99%

Gold Nanoparticles Enter Rat Ovarian Granulosa Cells and Subcellular Organelles, and Alter <i>In-Vitro</i> Estrogen Accumulation

Stelzer

Hutz

2009

Journal of Reproduction and Development

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“…57 After uptake, nanoparticles can disseminate to different body tissues. [58][59][60][61] However, few toxicology studies are presently available.…”

Section: Toxicitymentioning

confidence: 99%

Potential use of silver nanoparticles on pathogenic bacteria, their toxicity and possible mechanisms of action

Durán¹,

Marcato²,

Conti³

et al. 2010

J. Braz. Chem. Soc.

411

224

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As propriedades da prata são conhecidas há muitos anos. Recentemente, as nanopartículas de prata têm chamado a atenção por sua atividade antimicrobiana que oferece a possibilidade de uso com propósitos médicos e de higiene. Estas nanopartículas de prata em diferentes formulações, com diferentes formas e tamanhos, exibem atividades antimicrobianas diferentes. Entretanto, os mecanismos da atividade antimicrobiana de íons e de nanopartículas, assim como sua toxicidade em tecidos humanos não estão totalmente esclarecidos. Esta revisão avalia o uso potencial de nanopartículas de prata no controle de patogênicos com ênfase sobre sua ação contra bactérias patogênicas, sua toxicidade e possíveis mecanismos de ação.The antimicrobial properties of silver have been known for thousands of years. Recently, silver nanoparticles have gained attention because of their antimicrobial activity which offers the possibility of their use for medical and hygiene purposes. Indeed, silver nanoparticles in different formulations and with different shapes and sizes exhibit variable antimicrobial activity. However, the mechanisms of antimicrobial activity of silver ions and silver nanoparticles, and their toxicity to human tissues are not fully characterized. This review evaluates the potential use of silver nanoparticles to control pathogens with emphasis on their action against pathogenic bacteria, their toxicity and possible mechanisms of action.

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“…Recent studies have shown that administration of NPs to mice results in their accumulation in the various tissues including the brain and the testis. This indicates that they easily pass through the blood-brain and bloodtestis barriers [7,8]. Not all NPs will necessarily demonstrate an adverse effect leading to toxicity.…”

Section: Introductionmentioning

confidence: 99%

The effect of zinc oxide nanoparticles on mouse spermatogenesis

Talebi

Khorsandi

Moridian³

2013

J Assist Reprod Genet

126

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Objective To evaluate the effects of zinc oxide nanoparticles on mouse spermatogenesis. Methods Thirty two adult male NMRI mice were used. Experimental Groups (ZNP-1-ZNP-3) received one of the following treatments daily for 35 days: 5, 50 and 300 mg/kg zinc oxide nanoparticles respectively. Control group received only distilled water. Epididymal sperm parameters, testicular histopathology, morphometric analysis and spermatogenesis assessments were performed for evaluation of the zinc oxide nanoparticles effects on testis. Results Epididymal sperm parameters including sperm number, motility and percentage of abnormality were significantly changed in 50 and 300 mg/kg zinc oxide nanoparticles treated mice (p<0.01). Histopathological criteria such as epithelial vacuolization, sloughing of germ and detachment were significantly increased in 50 and 300 mg/kg zinc oxide nanoparticles treated mice (p<0.001). 300 mg/kg zinc oxide nanoparticles induced formation of multinucleated giant cells in the germinal epithelium. 50 and 300 mg/kg zinc oxide nanoparticles also caused a significant decrease in seminiferous tubule diameter, seminiferous epithelium height and maturation arrest (p<0.001). Conclusion Zinc oxide nanoparticles act as testicular toxicant and further studies are needed to establish its mechanism of action upon spermatogenesis.

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Sodium Chloride Modified Silica Nanoparticles as a Non-Viral Vector with a High Efficiency of DNA Transfer into Cells

Cited by 71 publications

References 0 publications

Gold Nanoparticles Enter Rat Ovarian Granulosa Cells and Subcellular Organelles, and Alter <i>In-Vitro</i> Estrogen Accumulation

Gold Nanoparticles Enter Rat Ovarian Granulosa Cells and Subcellular Organelles, and Alter <i>In-Vitro</i> Estrogen Accumulation

Potential use of silver nanoparticles on pathogenic bacteria, their toxicity and possible mechanisms of action

The effect of zinc oxide nanoparticles on mouse spermatogenesis

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