Sodium chloride-induced aggregation of dipalmitoylphoshpatidylglycerol small unilamellar vesicles with varying amounts of incorporated cholesterol

Virden, Jud W.; Berg, John C.

doi:10.1021/la00042a007

Cited by 37 publications

(27 citation statements)

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“…The ζ -potential of 1 in sodium chloride aqueous solutions gradually increases from −42 to −28 mV with increased sodium chloride concentration, implying that the negative charge density at the surface of the self-assembly gradually decreased upon addition of sodium chloride. A similar feature was also reported in sodium chloride-induced aggregation of dipalmitoylphosphatidylglycerol vesicles [16].…”

Section: Resultssupporting

confidence: 84%

Sodium chloride-induced self-assembly of microfibers from nanofiber components

Iwaura

Minamikawa

Shimizu

2004

Journal of Colloid and Interface Science

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Section: Resultssupporting

confidence: 84%

Sodium chloride-induced self-assembly of microfibers from nanofiber components

Iwaura

Minamikawa

Shimizu

2004

Journal of Colloid and Interface Science

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“…The crystallization of hydrocarbon chains of saturated lipids can be hindered by the use of Chol, leading to a gel state system (88). Virden and Birg found that the increase in Chol concentration in unilamellar DPPG based liposomes resulted in decreased aggregation (89). Chol within a liposomal formulation has the potential to influence the in vitro drug release properties.…”

Section: Resultsmentioning

confidence: 99%

Freeze-Dried Targeted Mannosylated Selenium-Loaded Nanoliposomes: Development and Evaluation

et al. 2013

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Abstract. The aim of this investigation was to develop and evaluate freeze-dried mannosylated liposomes for the targeted delivery of selenium. Dipalmitoylphosphatidylcholine, distearoylphosphatidylglycerol, and cholesterol were dissolved in a chloroform and methanol mixture and allowed to form a thin film within a rotatory evaporator. This thin film was hydrated with a sodium selenite (5.8 μM) solution to form multilamellar vesicles and homogenized under high pressure to yield unilamellar nanoliposomes. Seloaded nanoliposomes were mannosylated by 0.1%w/v mannosamine (Man-Lip-Se) prior to being lyophilized. Mannosamine concentration was optimized with cellular uptake studies in M receptor expressing cells. Non-lyophilized and lyophilized Man-Lip-Se were characterized for size, zeta potential, and entrapment efficiency. The influence of liposomal composition on the characteristics of Man-Lip-Se were evaluated using acidic and basic medium for 24 h. Thermal analysis and powder X-ray diffraction were used to determine the interaction of components within the Man-Lip-Se. The size, zeta potential and entrapment efficiency of the optimum Man-Lip-Se were observed to be 158±28.9 nm, 33.21±0.89 mV, and 77.27±2.34%, respectively. An in vitro Se release of 70-75% up to 24 h in PBS pH 6.8 and <8% Se release in acidic media (0.1 N HCl) in 1 h was observed. The Man-Lip-Se were found to withstand gastric-like environments and showed sustained release. Stable freeze-dried Man-Lip-Se were successfully formulated with a size of <200 nm, ∼75% entrapment, and achieved controlled release of Se with stability under acidic media, which may be of importance in the targeted delivery of Se to the immune system.

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“…Numerous studies on the use of cholesterol as a stabilizer were conducted, showing that this steroid can (1) increase the packing of phospholipid molecules [13], (2) reduce bilayer permeability to non-electrolyte and electrolyte solutes [14], (3) improve vesicle resistance to aggregation [15], (4) change the fluidity of intravesicle interactions to make them more rigid and sustain in severe shear stress of the lipid bilayer [16] and (5) reduce drug incorporation efficiency [17]. The maximum amount of cholesterol that has been reported and can be incorporated into reconstituted bilayers is widely assumed to be about 50 mol% [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Influence of cholesterol on liposome stability and on in vitro drug release

Briuglia

Rotella

McFarlane

et al. 2015

Drug Deliv. and Transl. Res.

521

268

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Cholesterol plays a strategic role in liposome composition; however, the quantity used to achieve an appropriate formulation has not been yet clarified. Therefore, by screening arrangement of lipids and cholesterol ratio, the main aim of this study is to investigate the most suitable amount of cholesterol in lipids in order to prepare stable and controlled drug release vehicles. For the preparation of liposomes, DMPC, DPPC and DSPC phospholipids were used and combined with different molar ratios of cholesterol (e.g. 100, 80-20, 70-30, 60-40 and 50-50%). Stability studies were conducted by storing the formulations at 37 and 50 °C for 30 days and by analysing them by AFM, DLS and FT-IR. By detecting the two most stable formulations from the stability results, drug encapsulation and in vitro release studies in PBS were performed by encapsulating atenolol and quinine. The release results were validated using a simulation model to ensure the reliability and suitable interpretation of the data. The generated model showed a good correlation between the prediction and the in vitro obtained results. By using 70:30% ratio (known in literature as 2:1), it is possible to reach the most stable formulation to guarantee a controlled and reproducible release for drugs with different physicochemical characteristics and pharmaceutical applications.

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Sodium chloride-induced aggregation of dipalmitoylphoshpatidylglycerol small unilamellar vesicles with varying amounts of incorporated cholesterol

Cited by 37 publications

References 0 publications

Sodium chloride-induced self-assembly of microfibers from nanofiber components

Sodium chloride-induced self-assembly of microfibers from nanofiber components

Freeze-Dried Targeted Mannosylated Selenium-Loaded Nanoliposomes: Development and Evaluation

Influence of cholesterol on liposome stability and on in vitro drug release

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