Sodium Butyrate Upregulates Kupffer Cell PGE2Production and Modulates Immune Function

Perez, Richard V; Stevenson, Frazier T.; Johnson, Jeremy; Morgan, M.; Erickson, Kent L.; Hubbard, Neil E.; Morand, Larry Z.; Rudich, Steven M.; Katznelson, S.; German, J. Bruce

doi:10.1006/jsre.1998.5316

Cited by 32 publications

(24 citation statements)

References 22 publications

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“…Uma vez que a composição nutricional e de energia das misturas de acidificantes foi utilizada na formulação das dietas experimentais, benefícios de um aporte nutricional extra desses acidificantes são improváveis, o que corrobora ações de controle antimicrobiano e trófico a partir da inclusão dessas substâncias nas dietas (van der Wielen et al, 2000;Sakata, 1987;Leeson et al, 2005). Outras ações podem ter auxiliado na resposta positiva dos acidificantes, como a modulação da função imune que favorece a digestão e absorção de nutrientes (Curi et al, 1993, Perez et al, 1998.…”

Section: Resultsunclassified

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Suplementação de acidificantes orgânicos e inorgânicos em dietas para frangos de corte: desempenho zootécnico e morfologia intestinal

Viola

Vieira

2007

R. Bras. Zootec.

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RESUMO -Avaliou-se o efeito da suplementação de acidificantes na dieta sobre o desempenho zootécnico e a morfologia intestinal de frangos de corte. Foram utilizadas 2.112 aves distribuídas em oito tratamentos, em seis repetições, constituídos da adição de antibióticos ou de misturas de acidificantes, de acordo com a fase de desenvolvimento (1 a 7, de 8 a 21 e de 22 a 35 dias de idade), em diferentes composições: controle positivo = bacitracina metil dissilicato (BMD -55 ppm) + colistina (10 ppm); controle negativo = sem antibióticos promotores de crescimento ou acidificantes; mistura A = 8, 4,5 e 2,5 kg/t; mistura B = 10, 6 e 3 kg/t; mistura C = 4, 2 e 1 kg/t; mistura C = 3, 3 e 1 kg/t; mistura D = 5; 2,5 e 1 kg/t; e mistura D = 3, 3 e 1 kg/t. Com exceção da mistura B, houve um benefício geral do uso de acidificantes sobre a conversão alimentar, que foi similar à obtida com inclusão de antibióticos. O intestino delgado das aves do controle negativo foi mais pesado e teve altura de vilosidades menor. A profundidade de cripta de Lieberkuhn não foi alterada pelo uso de antibióticos e pela maior parte dos acidificantes. Com exceção da mistura B, a utilização de acidificantes foi eficiente na manutenção do desempenho e das condições morfológicas do intestino delgado de frangos de corte, resposta similar aos benefícios obtidos com o uso de antibióticos. Não houve diferenças entre as misturas que possam ser atribuídas a diferentes composições, com exceção da maior profundidade de cripta duodenal das aves que receberam a mistura D (3/3/1 kg/t). control-negative = without antibiotic growth promoters or acidifiers; blend A = 8, 4.5 and 2.5 kg/t; blend B = 10, 6 and 3 kg/t; blend C = 4, 2 and 1 kg/t; blend C = 3, 3 and 1 kg/t; blend D = 5, 2.5 and 1 kg/t and blend D = 3, 3 and 1 kg/t. There was a general benefit with the use of acidifiers on feed conversion, which was similar to the use of antibiotics, except for blend B. Small intestine of birds of control-negative was heavier and had shorter villi height. The deeper Lieberkuhn crypt depth was not altered by the antibiots and by great part of the acidifiers. The use of acidifiers was efficient in maintenance of performance and morphological conditions of small intestines of broilers chicks, similar to the benefit of the use of antibiotics, except for Blend B. There was no difference among the blenders thea could be attributed the different composition, except for the duodenal crypt depth for birds fed Blend D (3/3/1 kg/t).Key Words: acidifier, broiler chicks, organic acid, small intestine morphology IntroduçãoOs programas de alimentação de frangos de corte têm tradicionalmente utilizado antibióticos como melhoradores do desempenho. No entanto, há um questionamento crescente dos consumidores em relação a essa prática. A União Européia proibiu o uso de antibióticos em inclusões baixas e constantes em alimentos para animais destinados ao consumo humano a partir de janeiro de 2006de janeiro de (EC, 2003. O uso de ácidos orgânicos e inorgânicos pode ser uma alternativa como ...

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Section: Resultsunclassified

“…Posteriormente, foram seccionados transversalmente em lâminas de 2 a 5 μm e corados com hematoxilina-eosina e examinados em microscopia ótica (Prophet et al, 1992).…”

Section: Methodsunclassified

Suplementação de acidificantes orgânicos e inorgânicos em dietas para frangos de corte: desempenho zootécnico e morfologia intestinal

Viola

Vieira

2007

R. Bras. Zootec.

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“…Previous work has demonstrated an increase in Kupffer cell prostaglandin E 2 production in response to sodium butyrate (53).…”

Section: Table III Proteins Of the Arachidonic Acid Metabolismmentioning

confidence: 93%

Warm Ischemia-induced Alterations in Oxidative and Inflammatory Proteins in Hepatic Kupffer Cells in Rats

Hirsch

Hansen

Choi

et al. 2006

Molecular & Cellular Proteomics

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The aim of the study was to investigate the impact of ischemia/reperfusion injury on the proteome of Kupffer cells. Lean Zucker rats (n ‫؍‬ 6 each group) were randomized to 75 min of warm ischemia or sham operation. After reperfusion for 8 h, Kupffer cells were isolated by enzymatic perfusion and density gradient centrifugation. Proteins were tryptically digested into peptides and differentially labeled with iTRAQ (isobaric tags for relative and absolute quantitation) reagent. After fractionation by cation exchange chromatography, peptides were identified by mass spectrometry (ESI-LC-MS/MS). Spectra were interrogated against the Swiss-Prot database and quantified using ProteinProspectorா. The results for heat shock protein 70 and myeloperoxidase were validated by ELISA. There is substantial evidence that indicates that Kupffer cells play a central role in the pathogenesis of liver parenchymal cell damage as seen during ischemia/reperfusion injury (1). The activation of Kupffer cells that occurs during ischemia results in the production of oxygen radicals and the modulation of proinflammatory and anti-inflammatory cytokines (2). These mediators are implicated in hepatic cell injury and sinusoidal function. It has been demonstrated that modulation of Kupffer cells can protect the liver (3, 4). The mechanisms that have been postulated include a decreased production of oxygen radicals and neutrophil infiltration. However, little is known about the differential effects of protein expression and changes in post-translational modifications in Kupffer cells that are exposed to warm ischemia. One objective of proteome research is to identify and describe the complex responses of an organism to different stimuli (5, 6). Rapid progress in both protein separation and identification techniques has made mass spectrometry a powerful tool for characterizing the protein content of a biological system (7-13).Isotope ratio mass spectrometry with isobaric tags for relative and absolute quantitation (iTRAQ) 1 allows for the quantification and identification of the protein content of up to four samples in one experiment by labeling all peptides in a given sample (14). Peptides of the samples are modified by incorporating the four different reagents. The derivatized peptides are indistinguishable in MS but exhibit intense low mass MS/MS signature ions that support quantitation.To our knowledge there have been no studies to date that have used a proteomic approach to study the effects of warm ischemia on protein expression in Kupffer cells. We hypothe- The abbreviations used are: iTRAQ, isobaric tags for relative and absolute quantitation; AIF-1, allograft inflammatory factor-1; C3, complement factor 3; C3a, activated C3; FDR, false discovery rate; IL, interleukin. Research

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“…COX enzymes play a critical role in converting arachidonic acid released from the cell membrane into prostaglandins. Perez et al [184] reported that butyrate upregulates Kupffer cell PGE2 production by upregulating inducible COX-2 mRNA levels and modulates immune function (Fig. 3).…”

Section: Scfas Induction Of Tregs By Prostaglandin E2 (Pge2)mentioning

confidence: 98%

Induction of regulatory T cells: A role for probiotics and prebiotics to suppress autoimmunity

Dwivedi

Kumar

Laddha³

et al. 2016

Autoimmunity Reviews

119

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Sodium Butyrate Upregulates Kupffer Cell PGE2Production and Modulates Immune Function

Cited by 32 publications

References 22 publications

Suplementação de acidificantes orgânicos e inorgânicos em dietas para frangos de corte: desempenho zootécnico e morfologia intestinal

Suplementação de acidificantes orgânicos e inorgânicos em dietas para frangos de corte: desempenho zootécnico e morfologia intestinal

Warm Ischemia-induced Alterations in Oxidative and Inflammatory Proteins in Hepatic Kupffer Cells in Rats

Induction of regulatory T cells: A role for probiotics and prebiotics to suppress autoimmunity

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