Sodium bicarbonate in seminal plasma stimulates the motility of mammalian spermatozoa through direct activation of adenylate cyclase.

Okamura, Nobuyuki; Tsutsumi, Yutaka; Soejima, Aya; Masuda, Hiroki; Sugita, Yoshiki

doi:10.1016/s0021-9258(17)39295-5

Cited by 357 publications

(34 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, other low molecular weight factors have also been found in hamster (Bavister & Yanagimachi, 1977) and pig (Okamura & Sugita, 1983) seminal plasma. These factors have been identified as taurine (Mrsny et al, 1979) and bicarbonate (Okamura et al, 1985), respectively. It is not clear whether the unknown factor described in this work relates to either of these: taurine does not stimulate fowl sperm motility at 40°C (G. J. Wishart & K. Ashizawa, unpublished observations); and the 'unknown' factor did not stimulate an increase in sperm cyclic AMP levels, the mechanism by which bicarbonate has been shown to act (Garbers, et al, 1982;Okamura & Sugita, 1983;Okamura et al, 1985).…”

Section: Discussionmentioning

confidence: 99%

Resolution of the sperm motility-stimulating principle of fowl seminal plasma into Ca2+ and an unidentified low molecular weight factor

Ashizawa

Wishart²

1987

Reproduction

View full text Add to dashboard Cite

It was confirmed, using an objective assay of motility, that fowl seminal plasma restores and stimulates the motility of fowl spermatozoa at 40 degrees C in a dose-dependent manner. By separation of a 100,000 g supernatant of fowl seminal plasma with Sephadex G-15, two peaks of motility-stimulating activity were distinguished. One peak coincided with that of calcium and was absent when calcium was removed from the seminal plasma with Dowex 50. The other peak, which accounted for 44% of motility-stimulating activity, contained a low molecular weight, dialysable factor which remains to be identified.

show abstract

Section: Discussionmentioning

confidence: 99%

Resolution of the sperm motility-stimulating principle of fowl seminal plasma into Ca2+ and an unidentified low molecular weight factor

Ashizawa

Wishart²

1987

Reproduction

View full text Add to dashboard Cite

show abstract

“…Unlike the previously identified tmACs, soluble AC activity was not associated with the plasma membrane, and it was insensitive to stimulation by G-proteins or the plant-derived pharmacological tmAC activator forskolin (FSK) [24,25]. A related activity was detected in spermatozoa, and this activity was stimulated by bicarbonate (HCO 3 − ) [26][27][28][29]. The molecular source of soluble AC activity remained elusive until, in 1999, our laboratory purified sAC protein from 950 rat testes [30,31].…”

Section: History Of Soluble Adenylyl Cyclasementioning

confidence: 99%

“…Beginning with ejaculation and continuing during transit through the female reproductive tract, sperm acquire the ability to fertilize an egg through a maturation process called capacitation [55]. Capacitation, which is essential for successful fertilization, is induced by HCO 3 − and dependent upon cAMP signalling [27]. As mentioned above, sAC was originally purified from 950 rat testes [30] and is known to be highly expressed in testis, specifically in male germ cells [56], and in sperm [37,57,58].…”

Section: Bicarbonate Stimulation: Mechanism Of Actionmentioning

confidence: 99%

Bicarbonate, carbon dioxide and pH sensing via mammalian bicarbonate-regulated soluble adenylyl cyclase

et al. 2021

View full text Add to dashboard Cite

Soluble adenylyl cyclase (sAC; ADCY10) is a bicarbonate (HCO 3 − )-regulated enzyme responsible for the generation of cyclic adenosine monophosphate (cAMP). sAC is distributed throughout the cell and within organelles and, as such, plays a role in numerous cellular signalling pathways. Carbonic anhydrases (CAs) nearly instantaneously equilibrate HCO 3 − , protons and carbon dioxide (CO 2 ); because of the ubiquitous presence of CAs within cells, HCO 3 − -regulated sAC can respond to changes in any of these factors. Thus, sAC can function as a physiological HCO 3 − /CO 2 /pH sensor. Here, we outline examples where we have shown that sAC responds to changes in HCO 3 − , CO 2 or pH to regulate diverse physiological functions.

show abstract

“…Establishing an acidic pH and a low bicarbonate concentration in the epididymal lumen keeps sperm in a quiescent state (Babcock et al 1983;Acott and Carr 1984;Okamura et al 1985;Tajima et al 1987;Holm and Wishart 1998;Gatti et al 1993). Changes in bicarbonate composition in the lumen trigger sperm motility and activation of their acrosomal enzymes, leading to sperm death (Lee and Storey 1986).…”

Section: Figures 2 Andmentioning

confidence: 99%

Region- and Cell-specific Differences in the Distribution of Carbonic Anhydrases II, III, XII, and XIV in the Adult Rat Epididymis

Hermo

Chong

Moffatt

et al. 2005

J Histochem Cytochem.

View full text Add to dashboard Cite

We employed RT-PCR followed by light microscope immunocytochemistry on St. Marie's- and Bouin's-fixed tissues to define the distribution of carbonic anhydrase (CA) isoforms in the male reproductive tract. The data revealed that CA II, III, IV, XII, and XIV were expressed in rat epididymis. Whereas CA III was found in principal cells of all epididymal regions, CA II was localized in narrow cells of the initial segment and principal cells of all regions. CA XII expression was most intense in the corpus and proximal cauda regions, where it appeared over the basolateral plasma membranes of principal cells. Narrow cells of the initial segment also revealed intense reactions, as did basal cells of the corpus and proximal cauda regions. Principal cells of the initial segment and proximal caput regions showed diffuse apical cytosolic reactions and occasional basolateral staining for CA XIV, whereas principal cells of distal regions showed more diffuse cytosolic reactions highlighting both apical and basal regions of the cell, with basal cells also being reactive. These data suggest subtle differences in cell type and subcellular- and region-specific distributions for CAs in their role of fine-tuning pH in the lumen, cell cytosol, and intervening intercellular spaces of the epididymis.

show abstract

Sodium bicarbonate in seminal plasma stimulates the motility of mammalian spermatozoa through direct activation of adenylate cyclase.

Cited by 357 publications

References 20 publications

Resolution of the sperm motility-stimulating principle of fowl seminal plasma into Ca2+ and an unidentified low molecular weight factor

Resolution of the sperm motility-stimulating principle of fowl seminal plasma into Ca2+ and an unidentified low molecular weight factor

Bicarbonate, carbon dioxide and pH sensing via mammalian bicarbonate-regulated soluble adenylyl cyclase

Region- and Cell-specific Differences in the Distribution of Carbonic Anhydrases II, III, XII, and XIV in the Adult Rat Epididymis

Contact Info

Product

Resources

About