Resolution of the sperm motility-stimulating principle of fowl seminal plasma into Ca2+ and an unidentified low molecular weight factor

Ashizawa, Koji; Wishart, G. J.

doi:10.1530/jrf.0.0810495

Cited by 68 publications

(14 citation statements)

References 15 publications

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“…Sperm motility is regulated by various factors such as osmotic changes[14], cations[15], steroids[16,17] and peptides/proteins[4,18–23], each of which acts in a highly adaptive manner to species-specific fertilization environment. In the internal fertilization, an endopeptidase and progesterone are reported to initiate and hyperactivate sperm motility in the female reproductive tracts of silkworm and human, respectively[17,20].…”

Section: Discussionmentioning

confidence: 99%

A Novel Cysteine Knot Protein for Enhancing Sperm Motility That Might Facilitate the Evolution of Internal Fertilization in Amphibians

et al. 2016

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Internal fertilization ensures successful reproduction of tetrapod vertebrates on land, although how this mode of reproduction evolved is unknown. Here, we identified a novel gene encoding sperm motility-initiating substance (SMIS), a key protein for the internal fertilization of the urodele Cynops pyrrhogaster by Edman degradation of an isolated protein and subsequent reverse transcription polymerase chain reaction. The SMIS gene encoded a 150 amino-acid sequence including the cysteine knot (CK) motif. No gene with substantial similarity to the SMIS was in the data bank of any model organisms. An active site of the SMIS was in the C-terminal region of the 2nd loop of CK motif. A synthetic peptide including the active site sequence bound to the midpiece and initiated/enhanced the circular motion of C. pyrrhogaster sperm, which allows penetration of the egg jelly specialized for the internal fertilization of this species. The synthetic peptide bound to whole sperm of Rhacophorus arboreus and enhanced the rotary motion, which is adapted to propel the sperm through egg coat matrix specialized for arboreal reproduction, while it bound to the tip of head and tail of Bufo japonicus sperm, and enhanced the vibratory motion, which is suited to sperm penetration through the egg jelly specialized for the reproduction of that species in freshwater. The polyclonal antibody against the active site of the SMIS specifically bound to egg coat matrix of R. arboreus. These findings suggest that diversification of amphibian reproductive modes accompanies the specialization of egg coat and the adaptation of sperm motility to penetrate the specialized egg coat, and SMIS acts as the sperm motility enhancer of anurans and urodeles that might facilitate to adaptively optimize sperm motility for allowing the establishment of internal fertilization.

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Section: Discussionmentioning

confidence: 99%

A Novel Cysteine Knot Protein for Enhancing Sperm Motility That Might Facilitate the Evolution of Internal Fertilization in Amphibians

et al. 2016

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“…The third implication of our experimental results pertains to the study of fowl sperm motility itself, which has focused on the axoneme [21][22][23][24][25][26][27][28][29][30] and the role of second messengers, extracellular Ca 2 + in particular [31][32][33][34][35][36]. To date, biochemical data, including that shown in Table 3, reflect averaged values.…”

Section: Discussionmentioning

confidence: 99%

Sperm Mobility: A Quantitative Trait of the Domestic Fowl (Gallus domesticus)1

Froman

Feltmann

1998

Biology of Reproduction

129

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Sperm from each rooster within a base population (n = 271) were evaluated with a mobility assay validated in previous work. Frequency analysis confirmed a normal distribution for the variable of sperm mobility. Repeated-measure analysis of males categorized by phenotype demonstrated that average and high sperm mobility phenotypes were distinct and independent of time. Sperm morphology, fertilizing ability, and ATP content were compared between phenotypes. Fertility and sperm ATP content differed (p < 0.001) between phenotypes, whereas sperm morphology did not (p > 0.05). Experimentation with washed sperm demonstrated that phenotype was fully expressed when mitochondrial respiration was the only source of ATP. Sperm mobility increased (p < 0.001) when sperm from average males were exposed to calyculin A, a protein phosphatase inhibitor. Correlation analyses were performed with data from a subpopulation (n = 46) whose range, mean, and variance were equivalent to those of the base population. Neither body weight nor the combined weight of the testes was correlated with sperm mobility (r = -0.02 and 0.01, respectively). In contrast, sperm ATP content was correlated with sperm mobility (r = 0.80). We attribute phenotypic differences in sperm mobility to differential rates of mitochondrial ATP synthesis.

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“…The activation of sperm motility depends on intracellular and extracellular calcium (Ca 2+ ) concentrations (Howarth, 1984;Ashizawa and Wishart, 1987;Thomason andWishart, 1989, 1991;Ashizawa et al, 1992Ashizawa et al, , 1994 as well as extracellular sodium (Na + ) and potassium (K + ) concentrations (Yassen and Foote, 1967;Cummings and Huston, 1976;Thomson and Wishart, 1991;Darszon et al, 1999). Cummings and Huston (1976) determined that ion concentrations increased in whole semen when birds were heat stressed, possibly because of an increase in The effects of heat stress and sperm quality classi cation on broiler breeder male fertility and semen ion concentrations † ‡ sperm concentration.…”

Section: Introductionmentioning

confidence: 99%

The effects of heat stress and sperm quality classification on broiler breeder male fertility and semen ion concentrations

Karaca

Parker²,

Yeatman³

et al. 2002

British Poultry Science

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1. The present study was undertaken to determine the effects of heat exposure on fertility, semen quality, and semen ion concentrations of broiler breeders classified on sperm quality index (SQI) before heat stress. 2. Cobb males (108) were individually caged in 6 temperature-controlled rooms. Each room contained an equal number of males from each of the 4 SQI population quartiles as follows: best (B), good (G), fair (F), and poor (P). Three rooms were heated to 35 degrees C, and the other three rooms were maintained at a constant 23 degrees C as controls. For each SQI group in each room, 15 Leghorn hens were artificially inseminated (5 x 10(7) sperm/hen) once a week for 8 weeks for fertility observations. 3. Body weight, sperm concentration, SQI, and fertility of P males were lower than in the other three SQI groups. Body temperature of the top three SQI groups was increased by heat exposure, but body temperature was not altered by heat stress in the P group. Fertility, sperm viability, and SQI of the top three SQI groups, but not the P group, was decreased by heat stress. Seminal plasma K+ of P males was lower than that of B males. However, seminal plasma Ca2+ concentration of P males was higher than that of B males. 4. In conclusion, high ambient temperatures had more impact on semen quality and fertility of males in the top 75% of the SQI population than in males in the bottom 25% of the population. In addition, calcium ions (Ca2+) appear to play a major role in heat stress infertility.

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Resolution of the sperm motility-stimulating principle of fowl seminal plasma into Ca2+ and an unidentified low molecular weight factor

Cited by 68 publications

References 15 publications

A Novel Cysteine Knot Protein for Enhancing Sperm Motility That Might Facilitate the Evolution of Internal Fertilization in Amphibians

A Novel Cysteine Knot Protein for Enhancing Sperm Motility That Might Facilitate the Evolution of Internal Fertilization in Amphibians

Sperm Mobility: A Quantitative Trait of the Domestic Fowl (Gallus domesticus)1

The effects of heat stress and sperm quality classification on broiler breeder male fertility and semen ion concentrations

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