Sodium alginate-grafted β-cyclodextrins as a matrix for immobilized Arthrobacter simplex for cortisone acetate biotransfromation

Shen, Yanbing; Niu, Ling; Yu, Ziqi; Wang, Min; Shang, Zhen; Yang, Yan

doi:10.1016/j.apsusc.2018.03.028

Cited by 17 publications

(4 citation statements)

References 27 publications

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“…Therefore, sodium alginate (NaAlg), a kind of natural polysaccharide, is an excellent carrier that meets the above conditions. It is widely used by immobilizers due to its easy availability, low cost, good biocompatibility, and ease of preparation [ 21 ]. In this study, biodegradable, inexpensive, and safe alginate and CaCl 2 were used to immobilize β-mannanase, and the optimal immobilization conditions on the immobilized enzyme were determined.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Enzymatic Performance of β-Mannanase Immobilized on Calcium Alginate Beads for the Generation of Mannan Oligosaccharides

Chen,

Tian,

Zhou

et al. 2023

Foods

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Mannan oligosaccharides (MOSs) are excellent prebiotics that are usually obtained via the enzymatic hydrolysis of mannan. In order to reduce the cost of preparing MOSs, immobilized enzymes that demonstrate good performance, require simple preparation, and are safe, inexpensive, and reusable must be developed urgently. In this study, β-mannanase was immobilized on calcium alginate (CaAlg). Under the optimal conditions of 320 U enzyme addition, 1.6% sodium alginate, 2% CaCl2, and 1 h of immobilization time, the immobilization yield reached 68.3%. The optimum temperature and pH for the immobilized β-mannanase (Man-CaAlg) were 75 °C and 6.0, respectively. The Man-CaAlg exhibited better thermal stability, a high degree of pH stability, and less substrate affinity than free β-mannanase. The Man-CaAlg could be reused eight times and retained 70.34% of its activity; additionally, the Man-CaAlg showed 58.17% activity after 30 days of storage. A total of 7.94 mg/mL of MOSs, with 4.94 mg/mL of mannobiose and 3.00 mg/mL of mannotriose, were generated in the oligosaccharide production assay. It is believed that this convenient and safe strategy has great potential in the important field of the use of immobilized β-mannanase for the production of mannan oligosaccharides.

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Section: Introductionmentioning

confidence: 99%

Enhanced Enzymatic Performance of β-Mannanase Immobilized on Calcium Alginate Beads for the Generation of Mannan Oligosaccharides

Chen,

Tian,

Zhou

et al. 2023

Foods

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“…侧链接枝型 CDP 主要包括将 CD 分子通过化学键接枝到合成高分子活性侧基上或生物大分子侧基上, 合成高分子类型有聚(甲基乙烯基醚-马来酸酐)共聚物 [54] 、聚丙烯酰胺(polyacrylamide) [55] 、聚丙烯酸(Poly(acrylic acid))及其嵌段共聚物 [56] 等; 天然高分子主要包括壳聚糖(Chitosan) [57] 、海藻酸钠(Sodium Alginate) [58] 等. 最近, 图 4 光驱动可解体的 CD 聚轮烷结构示意图 [50] Figure 4 Photo-reversible dissociation process of CD based PRs.…”

Section: 环糊精接枝聚合物(Grafted-cdp)unclassified

Advances in Cyclodextrin Polymers and Their Applications in Biomedicine

Qie¹,

Hao²,

Liu³

et al. 2020

Acta Chim. Sinica

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Cyclodextrins (CDs) are a family of macrocyclic oligosaccharides composed of α-1,4-linked D-glucopyranose units. The most commonly used CDs are α-, β-, and γ-CD, which consist of 6, 7, and 8 D-glucose units, respectively. They possess a relative hydrophobic inner cavity and a hydrophilic outer surface and can form inclusion complexes with various small molecules, metal ions and polymers, tailoring the physicochemical property of the guests, and thus have been widely used in the fields of pharmacy, food, chemistry, chromatography, catalysis, biotechnology, agriculture, cosmetics, hygiene, medicine, textiles and the environment, etc. However, it is difficult to manipulate the native CDs in some specific applications, they are crystallized solid and soluble in water but insoluble in most organic solvents. CD polymers (CDPs), such as crosslinked CDs or CD based hydrogels with various crosslinkers by chemical reactions, and CD based supramolecular polymers formed by physical interactions, can achieve the integration effect and synergy effect of CDs, crosslinkers and guest polymers, not only possessing the inclusion capacity of CDs, but also endowing CDs with other properties introduced by crosslinkers. The CDPs can be easily manipulated and they exhibit unique features that native CDs are lack of. Hence, the design, synthesis and applications of CDPs have attracted broad interests in recent years. This review focuses on the recent progress in CDPs, and different types of CDPs are identified and classified based on the structures and functions, namely CD based polyrotaxane, grafted CDs, crosslinked CDs and linear CDs, etc. Besides, the synthetic methodologies of CDPs are highlighted. Particular attention is paid to the breakthrough on the CDPs in the past five years, and their applications in biomedicine, such as drug delivery, gene delivery, target delivery, controlled release and cell imaging are discussed in detail. The typical applications in other fields such as absorption, environment remediation, thermal insulation, catalysis and slid-ring gels are also discussed in brief. Finally, the review provides brief summary and prospect of CDPs. Keywords cyclodextrin; polymer; chemical crosslinking; drug carrier; supramolecular self-assembly 1 引言环糊精(cyclodextrin, 简称 CD)是一类由吡喃葡萄糖单元通过 α-1,4-糖苷键链接而成的大环分子, 具有疏水空腔和亲水外壁的中空圆台状立体结构, 能够通过包结作用(encapsulation)与多种小分子和聚合物形成主-客体(host-guest)包结物(inclusion complexes), 从而实现对客体分子物理和化学性能的调控, 被广泛用于制药、食

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“…Sodium alginate (NaAlg), a biodegradable natural anionic polysaccharide, is mainly composed of α-L-gulonic aldehyde hydrochloride and β-D mannuronic aldehyde hydrochloride. Because of its biocompatibility, it was shown [ 4 ] to be a good material in encapsulation systems in early studies and to have a wide range of applications in the pharmaceutical and food fields. NaAlg, as an immobilizer has the advantages of low cost, wide availability, easy preparation, and good biocompatibility [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Thermodynamics and Physicochemical Properties of Immobilized Maleic Anhydride-Modified Xylanase and Its Application in the Extraction of Oligosaccharides from Wheat Bran

Zhao

Guo

et al. 2023

Foods

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Xylanases are the preferred enzymes for the extracting of oligosaccharides from wheat bran. However, free xylanases have poor stability and are difficult to reuse, which limit their industrial application. In the present study, we covalently immobilized free maleic anhydride-modified xylanase (FMA-XY) to improve its reusability and stability. The immobilized maleic anhydride-modified xylanase (IMA-XY) exhibited better stability compared with the free enzyme. After six repeated uses, 52.24% of the activity of the immobilized enzyme remained. The wheat bran oligosaccharides extracted using IMA-XY were mainly xylopentoses, xylohexoses, and xyloheptoses, which were the β-configurational units and α-configurational units of xylose. The oligosaccharides also exhibited good antioxidant properties. The results indicated that FMA-XY can easily be recycled and can remain stable after immobilization; therefore, it has good prospects for future industrial applications.

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Sodium alginate-grafted β-cyclodextrins as a matrix for immobilized Arthrobacter simplex for cortisone acetate biotransfromation

Cited by 17 publications

References 27 publications

Enhanced Enzymatic Performance of β-Mannanase Immobilized on Calcium Alginate Beads for the Generation of Mannan Oligosaccharides

Enhanced Enzymatic Performance of β-Mannanase Immobilized on Calcium Alginate Beads for the Generation of Mannan Oligosaccharides

Advances in Cyclodextrin Polymers and Their Applications in Biomedicine

Thermodynamics and Physicochemical Properties of Immobilized Maleic Anhydride-Modified Xylanase and Its Application in the Extraction of Oligosaccharides from Wheat Bran

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