SOD3 and eNOS genotypes are associated with SOD activity and NOx

Abstract

Oxidative stress, characterized by increased reactive oxygen species production and/or decreased antioxidant enzyme activity, plays an important role in the pathogenesis of hypertension. The identification of molecular markers corresponding to the oxidative stress status of hypertension may assist in the antioxidant therapy of hypertension. In the present study, superoxide dismutase (SOD) and endothelial nitric oxide synthase (eNOS) were analyzed as markers of hypertension responding to oxidative stress. The plasma SOD activity and mononitrogen oxides (NOx) concentration were measured, and the SOD3 Ala58Thr and eNOS Glu298Asp polymorphisms were genotyped in hypertensive patients and normotensive controls. Further association experiments were replicated in an extended population, including 343 hypertensive patients and 290 controls. The results demonstrated that no statistically significant differences in the total SOD activity and NOx concentration were identified between the hypertensive patients and controls. However, the plasma SOD activity levels in the SOD3 Ala/Ala homozygote carriers (80.51±27.68 U/ml) were significantly lower compared with the Thr allele carriers (92.18±16.37 U/ml; P=0.031). In addition, the plasma NOx concentration in the eNOS Glu/Glu homozygote carriers (129.66±59.15 μmol/l) was significantly lower compared with the Asp allele carriers (169.84± 55.18 μmol/l; P=0.010). Notably, the altered SOD activity levels and NOx concentration were in concordance in 56.3% of the 80 participants. Therefore, the concordance of decreased SOD activity and NOx concentration, combined with genotypes of SOD3 Ala/Ala and/or eNOS Glu/Glu in hypertensive patients, may be useful in directing the antioxidant therapy of hypertension.

Dietary copper affects antioxidant and immune activity in hybrid tilapia (Oreochromis niloticus × Oreochromis aureus)

“…Copper has been widely reported to affect antioxidant and immunological activities of organisms (Chen et al., ; Festa, Helsel, Franz, & Thiele, ; Guo, Miao, Xian, Qian, & Wang, ; Johnson et al., ). The levels of SOD, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and GSH were regarded as the biomarkers of oxidative stress (Dong, Li, Liu, & Zhao, ; Kumar, Swaminathan, Clemens, & Dey, ; Su et al., ). The cytokines interleukin‐1 (IL‐1) and tumour necrosis factor (TNF) are associated with immunological activities (Hogaboam, Snider, & Collins, ).…”

High Levels of Soluble Endoglin Induce a Proinflammatory and Oxidative-Stress Phenotype Associated with Preserved NO-Dependent Vasodilatation in Aortas from Mice Fed a High-Fat Diet

“…Indeed, endothelial dysfunction is accompanied with impaired nitric oxide (NO)-dependent vasodilatation, which is characterized by reduced endothelial NO synthase (eNOS) activity and/or enhanced inactivation of NO by increased oxidative stress [13,14,15]. In addition, increased levels of reactive oxygen species (ROS) contribute to endothelial dysfunction and vascular remodeling, which can be compensated by an increased expression of superoxide dismutase (SOD) to maintain the redox homeostasis as the first antioxidant defense system [16]. It should be noted that low expression of sEng is associated with decreased NO-dependent vasodilatation and lower expression of eNOS [17,18,19].…”

Actinidia chinensis Planch. Improves the Indices of Antioxidant and Anti‐Inflammation Status of Type 2 Diabetes Mellitus by Activating Keap1 and Nrf2 via the Upregulation of MicroRNA‐424

“…SOD [23, 24], AST [25, 26], ALT [27, 28], and GSH are associated with oxidative stress [29–34]. Here, the levels of SOD, AST, ALT, and GSH were measured.…”