Socio-Demographic Determinants Associated with Blastocystis Infection in Arequipa, Peru

Salazar-Sánchez, Renzo; Ascuña-Durand, Kasandra; Ballón-Echegaray, Jorge; Vásquez-Huerta, Victor; Martínez-Barrios, Elí; Castillo-Neyra, Ricardo

doi:10.4269/ajtmh.20-0631

Cited by 10 publications

(5 citation statements)

References 38 publications

(53 reference statements)

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“…in human fecal samples, in agreement with the results of previous studies [ 17 , 28 – 30 ]. Similarly, a detection rate of in vitro culture of 53%, 53.6%, and 51.3% were reported in previous studies [ 28 , 31 , 32 ]. On the other hand, other studies reported that PCR assay had the highest percentage of Blastocystis sp.…”

Section: Discussionsupporting

confidence: 82%

An Association Between Blastocystis Subtypes and Colorectal Cancer Patients: A Significant Different Profile from Non-cancer Individuals

et al. 2022

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Purpose Blastocystis is a common enteric human parasite of non-conclusive pathogenicity which may be determined by subtype (ST) variation. Colorectal cancer (CRC) is considered one of the primary causes of cancer mortality. Blastocystis ST7 has been shown to reduce beneficial intestinal microbiota and may exacerbate CRC. This study assessed the possible association between Blastocystis STs and CRC in comparison to non-cancer patients. Material and Methods A total of 200 fecal samples were obtained from CRC (100) and non-CRC (100) individuals attending Beni-Suef University Hospital, Egypt. Blastocystis was searched for in all samples using microscopy and culturing. Positive subculture samples were genetically sequenced and subtyped using conventional polymerase chain reaction (PCR). Blastocystis STs were determined by sequencing and a phylogenetic tree was created. Related patient characteristics and tumor stages were analyzed for association with presence of Blastocystis . Results Blastocystis was identified in 52% and 42% of CRC and non-cancer individuals, respectively. ST1, 2, and 3 were isolated from both cancer and non-cancer individuals; however, for the first time, ST7 was only isolated from CRC stool samples with significant association. Associated patient characteristics were evaluated as predictors. Conclusion Blastocystosis is highly prevalent in CRC patients, predominantly in the latest CRC grades and stages. To the best of our knowledge, this is the first study to report the identification of Blastocystis ST7 in CRC patients. To determine whether certain STs of Blastocystis are associated with CRC would require further research, including the role played by gut microbiota.

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Section: Discussionsupporting

confidence: 82%

An Association Between Blastocystis Subtypes and Colorectal Cancer Patients: A Significant Different Profile from Non-cancer Individuals

et al. 2022

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“…These observations support the historical findings on abundance of Blastocystis in developing countries owing to poor personal hygiene, unsanitary living conditions, consumption of contaminated food and water, and exposure to animals [9,27,28]. Moreover, the prevalence of Blastocystis reported in this study is consistent with previous studies done in developing world [4,29,30]. Blastocystis is transmitted through fecal-oral route.…”

Section: Discussionsupporting

confidence: 92%

Infection with Blastocystis spp. and its association with enteric infections and environmental enteric dysfunction among slum-dwelling malnourished adults in Bangladesh

et al. 2021

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Background Blastocystis spp. (Blastocystis) is a widely distributed gastrointestinal protist frequently reported in countries with tropical and sub-tropical climate. We sought to determine the factors associated with Blastocystis infection and investigate its role on biomarkers of intestinal health among slum-dwelling malnourished adults in Bangladesh. Methodology Total 524 malnourished adults with a body mass index ≤18.5 kg/m2 were included in this analysis. Presence of Blastocystis in feces was evaluated by TaqMan Array Card assays. Principal findings Blastocystis was tested positive in 78.6% of the participants. Prevalence of infection with atypical strains of enteropathogenic Escherichia coli (aEPEC) (56% vs. 38%, p<0.001), and Trichuris trichiura (28% vs. 15%, p-value = 0.02) was significantly greater in adults with Blastocystis, while Giardia intestinalis was significantly lower (8% vs. 14%, p-value = 0.04) in Blastocystis positive adults. Malnourished adults who were living in households with high crowding index (aOR = 2.18; 95% CI = 1.11, 4.65; p-value = 0.03), and infected with aEPEC (aOR = 2.14; 95% CI = 1.35, 3.44; p-value = 0.001) and Trichuris trichiura (aOR = 1.97; 95% CI = 1.08, 3.77; p = 0.03) were more likely to be infected with Blastocystis. A significant negative relationship was observed between Blastocystis and fecal concentrations of alpha-1 antitrypsin (β = -0.1; 95% CI = -1.7, -0.1; p-value<0.001) and Reg1B (β = -3.6; 95% CI = -6.9, -3.0; p-value = 0.03). Conclusions The study findings suggest that the presence of Blastocystis in human intestine influences gut health and may have potential pathogenic role in presence of other pathogens.

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“…It is possible that occupation may be a factor favouring the frequent infection of men. Additionally, other risk factors for blastocystosis was not considered in the present study, such as living with domestic animals or water being treated before consumption [36].…”

Section: Discussionmentioning

confidence: 99%

Blastocystis sp. Infection in Gabon: Prevalence and Association with Sociodemographic Factors, Digestive Symptoms and Anaemia

M’Bondoukwé¹,

Mawili-Mboumba²,

Ognagosso³

et al. 2023

AJPME

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Introduction: Blastocystis sp. is an intestinal protozoan that is commonly reported, but whose clinical significance remains controversial. Clinical forms of this infection range from asymptomatic carriage to clinical signs, specifically gastrointestinal ones. There is a lack of data on the epidemiology of this protist in Gabon. This study was carried out to provide data on the frequency of Blastocystis sp. infection and its association with clinical signs and the haemoglobin rate. Methods: A cross-sectional study was conducted between September 2018 and November 2019. Stool samples were collected in five of the nine provinces of Gabon from children and adults. Sociodemographic and clinical data were recorded using a standardised pre-tested questionnaire. Haematological parameters and temperature were reported in a laboratory register. Parasitological diagnosis was performed using stool direct examination and a Merthiolate-Iodine-Formaldehyde (M.I.F) concentration to detect Blastocystis sp. Results: In total, 843 participants were interviewed and examined; 414 brought back stool samples. The frequency of Blastocystis sp. infection was 45.2% (n = 187/414), and it increased with age: from 20.0% in young children to 49.5% in adults (P = 0.0057). Being a male (P = 0.08) tended to be associated with Blastocystis sp. carriage. In the multivariate logistic regression, only males were associated with Blastocystis sp. infection and had a 4.3-fold higher risk of being infected than females did (adjusted odds ratio = 4.3; 95% CI = 1.2–15.6; P = 0.03). Diarrhoea, abdominal pain and colitis were observed in some patients with Blastocystis sp. monoinfection. No relation between Blastocystis sp. carriage and anaemia was found. Conclusion: The frequency of Blastocystis sp. infection was high. Males were more at risk of being infected. Blastocystis sp. could be used as indicator in the improvement of environmental sanitation and hygiene, coupled with improved housing. Additional investigations in a population with clinical symptoms should be performed.

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Socio-Demographic Determinants Associated with Blastocystis Infection in Arequipa, Peru

Cited by 10 publications

References 38 publications

An Association Between Blastocystis Subtypes and Colorectal Cancer Patients: A Significant Different Profile from Non-cancer Individuals

An Association Between Blastocystis Subtypes and Colorectal Cancer Patients: A Significant Different Profile from Non-cancer Individuals

Infection with Blastocystis spp. and its association with enteric infections and environmental enteric dysfunction among slum-dwelling malnourished adults in Bangladesh

Blastocystis sp. Infection in Gabon: Prevalence and Association with Sociodemographic Factors, Digestive Symptoms and Anaemia

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