Social microbial inocula confer functional stability in a methyl tert-butyl ether extractive membrane biofilm bioreactor

Purswani, Jessica; Guisado, Isabel M.; Coello-Cabezas, Julio; González‐López, Jesús; Pozo, Clementina

doi:10.1016/j.envpol.2018.10.100

Cited by 9 publications

(1 citation statement)

References 31 publications

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“…For this, the Microtox ® M500 toxicity analyser (Instrumentación Analítica S.A. Madrid, Spain) was employed. Toxicity is expressed as EC 50 (%), the concentration of the sample that causes a 50% of bioluminescence reduction in the bacterium Aliivibrio fischeri after 5 and 15 min of exposure to the sample [ 32 , 33 ]. The analyses were performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Simultaneous Heavy Metal-Polycyclic Aromatic Hydrocarbon Removal by Native Tunisian Fungal Species

Hkiri

Olicón-Hernández

Pozo

et al. 2023

JoF

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Multi-contamination by organic pollutants and toxic metals is common in anthropogenic and industrial environments. In this study, the five fungal strains Chaetomium jodhpurense (MH667651.1), Chaetomium maderasense (MH665977.1), Paraconiothyrium variabile (MH667653.1), Emmia lacerata, and Phoma betae (MH667655.1), previously isolated in Tunisia, were investigated for the simultaneous removal and detoxification of phenanthrene (PHE) and benzo[a]anthracene (BAA), as well as heavy metals (HMs) (Cu, Zn, Pb and Ag) in Kirk’s media. The removal was analysed using HPLC, ultra-high performance liquid chromatography (UHPLC) coupled to a QToF mass spectrometer, transmission electron microscopy, and toxicology was assessed using phytotoxicity (Lepidium sativum seeds) and Microtox® (Allivibrio fisherii) assays. The PHE and BAA degradation rates, in free HMs cultures, reached 78.8% and 70.7%, respectively. However, the addition of HMs considerably affected the BAA degradation rate. The highest degradation rates were associated with the significant production of manganese-peroxidase, lignin peroxidase, and unspecific peroxygenase. The Zn and Cu removal efficacy was considerably higher with live cells than dead cells. Transmission electron microscopy confirmed the involvement of both bioaccumulation and biosorption processes in fungal HM removal. The environmental toxicological assays proved that simultaneous PAH and HM removal was accompanied by detoxification. The metabolites produced during co-treatment were not toxic for plant tissues, and the acute toxicity was reduced. The obtained results indicate that the tested fungi can be applied in the remediation of sites simultaneously contaminated with PAHs and HMs.

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Section: Methodsmentioning

confidence: 99%